The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Effects of Preservation Period at Low Temperature on the Mycelial Growth and the Lignocellulolytic Enzyme Activities of Basidiomycetes

저온보존기간이 담자균류의 균사생장과 목질분해효소의 활성에 미치는 영향

  • Jeong, Yeun Sug (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Ka, Kang-Hyeon (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute)
  • 정연석 (국립산림과학원 화학미생물과) ;
  • 가강현 (국립산림과학원 화학미생물과)
  • Received : 2014.11.28
  • Accepted : 2014.12.12
  • Published : 2014.12.30
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Abstract

Subculture is the most common method for preservation fungi, but has a disadvantage of accumulation of spontaneous mutations during the repeated subculture. To reduce the subculture frequency, the effect of preservation period at $4^{\circ}C$ in a slant culture was examined on the mycelia growth and lignocellulolytic enzyme activities of various basidiomycetes. Mushrooms, including Stereum ostrea, Coprinellus micaeus, Trametes versicolor, Hypholoma fasciculare, Wolfiporia extensa, Pleurotus pulmonarius, Piptoporus betulinus and Ganoderma applanatum were not affected by the preservation period more than two years, indicating that they can be maintained by subculture every two years. Some other tested fungal strains showed a significant decrease in both viability and enzyme activity when they were maintained for two years, suggesting that they should be subcultured at least once in a year. A little correlation was found between the recovery of mycelial growth and extracellular enzyme activity. In conclusion, mycelial activity and enzyme activity according to storage period is expected to be a way of deciding on subculture times for fungal preservation.

계대배양은 균류의 보존을 위해 가장 일반적 방법이지만, 반복된 계대배양을 하는 동안 저절로 변이들이 축적된다는 단점이 있다. 계대배양의 횟수를 줄이기 위해, 사면배지에서 $4^{\circ}C$로 보존하는 것의 효과를 다양한 균주들의 균사생장과 목질 분해효소의 활성을 통해 조사해보았다. 갈색꽃구름버섯, 갈색먹물버섯, 구름버섯, 노란개암버섯, 복령, 산느타리, 자작나무버섯, 잔나비불로초를 포함한 버섯들은 2년 이상의 보존기간에도 영향을 받지 않아서, 이는 2년에 한번 계대배양을 하여도 유지가 가능하다는 것을 의미한다. 그 이외의 실험 균주들은 2년이 지났을 경우 활력과 효소의 활성이 크게 감소한 것으로 나타났으며 1년에 한 번 계대배양 하는 것이 좋을 것으로 판단되었다. 균사 생장력의 회복과 세포외 효소의 활성은 약간의 상관관계가 나타났다. 결론적으로, 보존기간에 따른 균사활력 및 효소의 활성은 균류 보존의 횟수를 정하는 하나의 방법으로 사용될 수 있을 것으로 여겨진다.

Keywords

References

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