The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Endophytic Fungal Diversity Isolated from the Root of Halophytes in Taean Peninsula

태안반도에 자생하는 염생식물의 뿌리로부터 분리한 내생 진균의 다양성

  • You, Young-Hyun (School of Life Science, Kyungpook National University) ;
  • Lee, Myung-Chul (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Kim, Jong-Guk (School of Life Science, Kyungpook National University)
  • Received : 2014.07.29
  • Accepted : 2014.11.23
  • Published : 2014.12.30
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Abstract

Halophytes of seven species, Carex scabrifolia Steud., Limonium tetragonum Bullock, Salicornia europaea L., Suaeda glauca Bunge, Suaeda japonica Makino, Suaeda maritima Dumort., and Triglochin maritimum L. were collected from the Taean Peninsula. Thirty-seven endophytic fungi were isolated from the root of halophytes, and analyzed using the DNA sequences of internal transcribed spacers (ITS). The diversity of all endophytic fungi was analyzed using diversity indices. The endophytic fungi associated with the halophytes belonged to seven orders; Eurotiales (78%), Capnodiales (5%), Hypocreales (5%), Agaricales (3%), Corticiales (3%), Glomerellales (3%), and Pleosporales (3%). At the phylum level, the endophytic fungi were composed of Ascomycota and Basidiomycota. At the genus level, Alternaria, Aspergillus, Cladosporium, Paecilomyces, Penicillium, Phanerochaete, Schizophyllum, Talaromyces, and Verticillium were confirmed. Among them, Penicillium was the most abundant in the roots of the halophytes. This study analyzed the distribution and diversity of endophytic fungi on halophytes in the Taean Peninsula.

7종의 자생 염생식물은 천일사초, 갯질경, 퉁퉁마디, 나문재, 칠면초, 해홍나물, 그리고 지채로서 태안반도에서 채집되었다. 염생식물의 뿌리로부터 분리된 37균주의 내생진균은 ITS영역 염기서열에 대하여 분석하였다. 모든 내생진균들은 다양성 지수들을 이용하여 분석되었다. 분리된 내생진균은 Eurotiales (78%), Capnodiales (5%), Hypocreales (5%), Agaricales (3%), Corticiales (3%), Glomerellales (3%), Pleosporales (3%)으로 7개 목으로 분류되었다. 그리고 분리된 내생진균의 속을 분석한 결과 자낭균문과 담자균문에 포함되는 Alternaria, Aspergillus, Cladosporium, Paecilomyces, Penicillium, Phanerochaete, Schizophyllum, Talaromyces, Verticillium등 9개 속으로 분류되었다. 내생진균을 분석하였을 때, Penicillium이 염생식물로부터 가장 많이 분포하고 있었다. 본 연구는 태안반도에 자생하는 염생식물들에 대한 내생진균의 분포와 다양성을 분석하였다.

Keywords

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