Mycobiology

  • 제42권4호
  • /
  • Pages.409-411
  • /
  • 2014
  • /
  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

The First Report of Postharvest Stem Rot of Kohlrabi Caused by Sclerotinia sclerotiorum in Korea

  • Kim, Joon-Young (Department of Plant Science, Gangneung-Wonju National University) ;
  • Aktaruzzaman, Md. (Department of Plant Science, Gangneung-Wonju National University) ;
  • Afroz, Tania (Department of Plant Science, Gangneung-Wonju National University) ;
  • Hahm, Young-Il (Daegwallyong Horticultural Cooperative) ;
  • Kim, Byung-Sup (Department of Plant Science, Gangneung-Wonju National University)
  • 투고 : 2014.09.10
  • 심사 : 2014.10.13
  • 발행 : 2014.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In March 2014, a kohlrabi stem rot sample was collected from the cold storage room of Daegwallyong Horticultural Cooperative, Korea. White and fuzzy mycelial growth was observed on the stem, symptomatic of stem rot disease. The pathogen was isolated from the infected stem and cultured on potato dextrose agar for further fungal morphological observation and to confirm its pathogenicity, according to Koch's postulates. Morphological data, pathogenicity test results, and rDNA sequences of internal transcribed spacer regions (ITS 1 and 4) showed that the postharvest stem rot of kohlrabi was caused by Sclerotinia sclerotiorum. This is the first report of postharvest stem rot of kohlrabi in Korea.

키워드

참고문헌

  1. Agrios GN. Plant pathology. 5th ed. San Francisco: Elsevier Academic Press; 2005. p. 546-50.
  2. Coley-Smith JR, Cooke RC. Survival and germination of fungal sclerotia. Annu Rev Phytopathol 1971;9:65-92. https://doi.org/10.1146/annurev.py.09.090171.000433
  3. Purdy LH. Sclerotinia sclerotiorum: history, diseases and symptomatology, host range, geographic distribution, and impact. Phytopathology 1979;69:875-80. https://doi.org/10.1094/Phyto-69-875
  4. Boland GJ, Hall R. Index of plant hosts of Sclerotinia sclerotiorum. Can J Plant Pathol 1994;16:93-108. https://doi.org/10.1080/07060669409500766
  5. Korean Society of Plant Pathology. List of plant diseases in Korea. 5th ed. Seoul: Korean Society of Plant Pathology; 2009. p. 7-667.
  6. Willetts HJ, Wong JAL. The biology of Sclerotinia sclerotiorum, S. trifoliorum, and S. minor with emphasis on specific nomenclature. Bot Rev 1980;46:101-65. https://doi.org/10.1007/BF02860868
  7. Chang SW, Kim SK. First report of Sclerotinia rot caused by Sclerotinia sclerotiorum on some vegetable crops in Korea. Plant Pathol J 2003;19:79-84. https://doi.org/10.5423/PPJ.2003.19.2.079
  8. Huang HC, Kozub GC. A simple method for production of apothecia from sclerotia of Sclerotinia sclerotiorum. Plant Prot Bull Taiwan 1989;31:333-45.
  9. Hao JJ, Subbarao KV, Duniway JM. Germination of Sclerotinia minor and S. sclerotiorum sclerotia under various soil moisture and temperature combinations. Phytopathology 2003;93:443-50. https://doi.org/10.1094/PHYTO.2003.93.4.443
  10. Moller EM, Bahnweg G, Sandermann H, Geiger HH. A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Res 1992;20:6115-6. https://doi.org/10.1093/nar/20.22.6115
  11. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
  12. Kohn LM. A monographic revision of the genus Sclerotinia. Mycotaxon 1979;9:365-444.
  13. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9. https://doi.org/10.1093/molbev/msr121
  14. Nishizawa T, Zhaorigetu, Komatsuzaki M, Sato Y, Kaneko N, Ohta H. Molecular characterization of fungal communities in non-tilled, cover-cropped upland rice field soils. Microbes Environ 2010;25:204-10. https://doi.org/10.1264/jsme2.ME10108
  15. Whetzel HH. A synopsis of the genera and species of the Sclerotiniaceae, a family of stromatic inoperculate discomycetes. Mycologia 1945;37:648-714. https://doi.org/10.2307/3755132