Mycobiology

The Korean Society of Mycology (한국균학회)
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Incidence and Level of Aflatoxins Contamination in Medicinal Plants in Korea

  • Lee, Sung Deuk (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Yu, In Sil (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jung, Kweon (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Yeon Sun (Department of Public Health, Graduate School, Dankook University)
  • Received : 2014.08.01
  • Accepted : 2014.11.07
  • Published : 2014.12.31
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Abstract

During 2011~2013, a total of 729 samples for 19 types of medicinal plant were collected from Seoulyekryungsi in Seoul, Korea, and investigated for the presence of aflatoxins. The samples were analyzed using immunoaffinity column cleanup and high-performance liquid chromatography coupled to a fluorescence detector after post-column derivatization. Aflatoxins were found in 124 out of the 729 analyzed samples: 65 containing aflatoxin B1 (AFB1), 24 with aflatoxin B2 (AFB2), 15 with aflatoxin G1 (AFG1), and 20 samples with aflatoxin G2 (AFG2). The ranges for positive samples were $0.1{\sim}404.7{\mu}g/kg$ for AFB1, $0.1{\sim}10.0{\mu}g/kg$ for AFB2, $0.1{\sim}635.3{\mu}g/kg$ for AFG1, $0.1{\sim}182.5{\mu}g/kg$ for AFG2, and $0.1{\sim}1,043.9{\mu}g/kg$ for total aflatoxins. Most of the medicinal plant samples (721, 98.9%) were below legal limits, but 8 samples exceeded the legal limits of 10 and $15{\mu}g/kg$ established by the Korean standard for AFB1 and total aflatoxins (the sum of AFB1, AFB2, AFG1 and AFG2), respectively.

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References

  1. Korea Food and Drug Administration. Food code, KFDA notification No 2008-4. Seoul: Korea Food and Drug Administration; 2008.
  2. Korea Food and Drug Administration. Food code, KFDA notification No 2009-104. Seoul: Korea Food and Drug Administration; 2009.
  3. Korea Food and Drug Administration. Food code, KFDA notification No 2011-42. Seoul: Korea Food and Drug Administration; 2011.
  4. Saleemullah, Iqbal A, Khalil IA, Shah H. Aflatoxin contents of stored and artificially inoculated cereals and nuts. Food Chem 2006;98:699-703. https://doi.org/10.1016/j.foodchem.2005.06.034
  5. European Food Safety Authority. Opinion of the scientific panel on contaminants in the food chain on a request from the commission related to the potential increase of consumer health risk by a possible increase of the existing maximum levels for aflatoxins in almonds, hazelnuts and pistachios and derived products. No. ESFA-Q-2006-174. EFSA J 2007;446:1-127.
  6. Reiter E, Zentek J, Razzazi E. Review on sample preparation strategies and methods used for the analysis of aflatoxins in food and feed. Mol Nutr Food Res 2009;53:508-24. https://doi.org/10.1002/mnfr.200800145
  7. Midio AF, Campos RR, Sabino M. Occurrence of aflatoxins B1, B2, G1 and G2 in cooked food components of whole meals marketed in fast food outlets of the city of Sao Paulo, SP, Brazil. Food Addit Contam 2001;18:445-8.
  8. Speijers GJ, Speijers MH. Combined toxic effects of mycotoxins. Toxicol Lett 2004;153:91-8. https://doi.org/10.1016/j.toxlet.2004.04.046
  9. International Agency for Research on Cancer (IARC). Monographs on the evaluation of the carcinogenic risks of chemicals to humans. Some naturally occurring substances: food items and constituents: heterocyclic aromatic amines and mycotoxin. Vol. 56. IARC: Lyon; 1993. p. 245-521.
  10. Food and Agriculture Organization of United Nations (FAO). Manual on the application of the HACCP system in mycotoxin prevention and control, No. 73. FAO Food and Nutrition Paper. FAO: Rome; 2001.
  11. Cho SY, Kang SJ, Jung J, Jeong BO, Jeong CS. Co-contamination of aflatoxins with ochratoxin A and zearalenone in Thuja orientalis Semen. Toxicol Res 2009;25:125-31. https://doi.org/10.5487/TR.2009.25.3.125
  12. Bennett JW, Klich M. Mycotoxins. Clin Microbiol Rev 2003;16:497-516. https://doi.org/10.1128/CMR.16.3.497-516.2003
  13. European Pharmacopoeia. European Directorate for the Quality of Medicines and Health Care (EDQM). 7th ed. Strasbourg: Council of Europe; 2011.
  14. World Health Organization. WHO guidelines for assessing quality of herbal medicines with reference to contaminants and residues. Geneva: WHO; 2007.
  15. Ashiq S, Hussain M, Ahmad B. Natural occurrence of mycotoxins in medicinal plants: a review. Fungal Genet Biol 2014;66:1-10. https://doi.org/10.1016/j.fgb.2014.02.005
  16. Romagnoli B, Menna V, Gruppioni N, Bergamini C. Aflatoxins in spices, aromatic herbs, herb-teas and medicinal plants marketed in Italy. Food Control 2007;18:697-701. https://doi.org/10.1016/j.foodcont.2006.02.020
  17. Arranz I, Sizoo E, Van Egmond H, Kroeger K, Legarda TM, Burdaspal P, Reif K, Stroka J. Determination of aflatoxin B1 in medical herbs: interlaboratory study. J AOAC Int 2006;89: 595-605.
  18. Zhang X, Liu H, Chen J. Immunoaffinity column cleanup with liquid chromatography using post-column bromination for aflatoxins in medicinal herbs and plant extracts. J Chromatogr Sci 2005;43:47-51. https://doi.org/10.1093/chromsci/43.1.47
  19. Ip SP, Che CT. Determination of aflatoxins in Chinese medicinal herbs by high-performance liquid chromatography using immunoaffinity column cleanup improvement of recovery. J Chromatogr A 2006;1135:241-4. https://doi.org/10.1016/j.chroma.2006.10.025
  20. Rizzo I, Vedoya G, Maurutto S, Haidukowski M, Varsavsky E. Assessment of toxigenic fungi on Argentinean medicinal herbs. Microbiol Res 2004;159:113-20. https://doi.org/10.1016/j.micres.2004.01.013
  21. Roy AK, Chourasia HK. Mycoflora, mycotoxin producibility and mycotoxins in traditional herbal drugs from India. J Gen Appl Microbiol 1990;36:295-302. https://doi.org/10.2323/jgam.36.295
  22. Chourasia HK. Mycobiota and mycotoxins in herbal drugs of Indian pharmaceutical industries. Microbiol Res 1995;99:697-703.
  23. Tassaneeyakul W, Razzazi-Fazeli E, Porasuphatana S, Bohm J. Contamination of aflatoxins in herbal medicinal products in Thailand. Mycopathologia 2004;158:239-44. https://doi.org/10.1023/B:MYCO.0000041892.26907.b4
  24. Santos L, Marin S, Sanchis V, Ramos AJ. Screening of mycotoxin multicontamination in medicinal and aromatic herbs sampled in Spain. J Sci Food Agric 2009;89:1802-7. https://doi.org/10.1002/jsfa.3647
  25. Cotty PJ, Jaime-Garcia R. Influences of climate on aflatoxin producing fungi and aflatoxin contamination. Int J Food Microbiol 2007;119:109-15. https://doi.org/10.1016/j.ijfoodmicro.2007.07.060
  26. Food and Agriculture Organization of United Nations (FAO). Worldwide regulations for mycotoxins in food and feed in 2003. Rome: FAO; 2004.
  27. Reddy KR, Salleh B, Saad B, Abbas HK, Abel CA, Shier WT. An overview of mycotoxin contamination in foods and its implications for human health. Toxin Rev 2010;29:3-26. https://doi.org/10.3109/15569541003598553
  28. Liu L, Jin H, Sun L, Ma S, Lin R. Determination of aflatoxins in medicinal herbs by high-performance liquid chromatographytandem mass spectrometry. Phytochem Anal 2012;23:469-76. https://doi.org/10.1002/pca.2343
  29. Sweeney MJ, Dobson AD. Mycotoxin production by Aspergillus, Fusarium and Penicillium species. Int J Food Microbiol 1998;43:141-58. https://doi.org/10.1016/S0168-1605(98)00112-3