Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Occurrence of Tetracyclines Resistant Bacteria in the Soil Applied with Livestock Manure Compost

가축분 퇴비 시용 농경지 내 Tetracyclines 내성균 분포 특성

  • Kim, Song-Yeob (Division of Applied Life Science (BK21plus program)) ;
  • Kim, Jang Hwan (Division of Applied Life Science (BK21plus program)) ;
  • Kim, Sung Chul (Department of Bio Environmental Chemistry, Chungnam National University) ;
  • Lee, Yong-Bok (Research Institute of Life Science, Gyeongsang National University)
  • 김송엽 (경상대학교 응용생명과학부) ;
  • 김장환 (경상대학교 응용생명과학부) ;
  • 김성철 (충남대학교 농업생명과학대학 생물환경화학과) ;
  • 이용복 (경상대학교 생명과학연구원)
  • Received : 2014.10.24
  • Accepted : 2014.11.12
  • Published : 2014.12.31
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Abstract

BACKGROUND: Large amount of veterinary antibiotics have been used in the livestock industry to prevent diseases and promote growth. These antibiotics are excreted through feces and urine in unchanged form and reach to agricultural fields via application of the livestock manure based composts. The purpose of this study was to evaluate the occurrence of tetracyclines-resistant bacteria in the soil received livestock manure compost for a long term. METHODS AND RESULTS: Tetracyclines (tetracycline TC, chlortetracycline CTC, and oxytetracycline OTC) resistance bacteria in the soil of rice-onion field applied pig manure compost (PM), in the soil of grass-rye field received cow manure compost (CM), and in the soil of rice field applied inorganic fertilizer (NPK) were determined. The soil received livestock manure composts clearly showed higher number of TC, CTC, and OTC resistance bacteria compared with the soil treated with inorganic fertilizer. The antibiotic resistant bacteria recovered appeared at 80 mg/L of tetracyclines was identified 1 specie, 6 genera 7 species, and 6 genera 7species in the soils received CM, PM, and NPK, respectively. The dominant resistant bacteria with the CM and PM application were Ochrobactrum and Rhodococcus. CONCLUSION: The application of livestock manure compost in the agricultural field is likely to contribute the occurrence of antibiotic resistance bacteria in the agricultural environment.

Keywords

References

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