한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제43권12호
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  • Pages.1801-1807
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  • 2014
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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동결건조 진주담치 추출물의 항산화 및 DNA 손상 보호 활성

Antioxidant and DNA Damage Protective Activities of Freeze-Dried Blue Mussel (Mytilus edulis)

  • Lee, Seon Woo (Department of Food and Nutrition, Kyungnam University) ;
  • Choi, Mi-Joo (Department of Food and Nutrition, Kyungnam University) ;
  • Kim, Si-Kyung (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Seung-Cheol (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Eunju (Department of Food and Nutrition, Kyungnam University)
  • 투고 : 2014.09.12
  • 심사 : 2014.11.23
  • 발행 : 2014.12.31
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초록

동결건조에 의한 진주담치의 생리활성의 변화를 살펴보고자 DPPH 라디칼 소거능, ORAC, CAC 등의 항산화 활성과 comet assay를 이용한 DNA 손상 보호능을 측정하였다. 생 진주담치 및 동결건조 진주담치에서 물 추출물을 제외한 에탄올과 메탄올 추출물에서 DPPH 라디칼 소거능을 확인하였고, 메탄올 추출물의 경우 동결건조에 의해 DPPH 라디칼 소거능이 증가하는 것으로 나타났다. 생 진주담치의 ORAC 수치는 물 추출물에서 가장 높게 나타난 반면, 동결건조 진주담치의 경우 메탄올 추출물에서 가장 높게 나타났다. 동결건조 후 ORAC 수치는 물 추출물에서만 유의적으로 감소된 반면 HepG2 세포의 라디칼 소거능(CAC)의 경우 물 추출물에서 유의적으로 증가하여 소거 대상 라디칼에 따라 항산화 활성의 차이가 있는 것으로 나타났다. 생 또는 동결건조 진주담치는 모든 추출물에서 산화적 스트레스에 의한 DNA 손상을 억제하는 보호 효과가 있음이 밝혀졌고 동결건조에 의한 보호 효과는 유의적인 변화가 없는 것으로 나타났다. 결론적으로 진주담치는 물 추출물의 DPPH 라디칼 소거능을 제외하고는 모든 추출물에서 항산화 활성과 더불어 DNA 손상의 보호 효과가 관찰되었고 동결건조 가공처리에 의해서 그 활성이 크게 영향을 받지 않거나 추출물에 따라 오히려 활성이 증가함을 알 수 있었다. 이러한 결과를 바탕으로 우리나라에서 생산되는 진주담치의 식품 첨가물이나 기능성 식품 개발을 위한 생리활성 소재로의 가능성을 확인할 수 있었다.

Blue mussels (Mytilus edulis) are widely distributed among the world's oceans in various habitats. The purpose of this study was to investigate the effects of freeze-drying on the antioxidant and antigenotoxic activities of blue mussels collected in the Gyeongnam coast area of Korea. Raw (RM) and freeze-dried blue mussel flesh (FRM) were extracted with ethanol, methanol, and water. Antioxidant activities were evaluated on the basis of DPPH radical scavenging activity, oxygen radical absorbance capacity (ORAC), cellular antioxidant capacity (CAC), and antigenotoxic activity (comet assay). Except for the water extract, RM and FRM showed DPPH radical scavenging activities, which increased upon freeze-drying in MeOH extract. The highest ORAC value was observed in water extract of RM and MeOH extract of FRM. CAC was protected against AAPH-induced oxidative stress in HepG2 cells by both RM and FRM extracts. Freeze-drying lowered ORAC value of water extract, whereas it increased CAC activity, suggesting that antioxidant activities varied according to the generated radicals. All extracts from RM and FRM showed antigenotoxic activities by reducing $H_2O_2$-induced DNA damage in human leukocytes. Freeze-drying had no effect on antigenotoxicity of blue mussels. Taken together, these results indicate that blue mussels possess antioxidant and antigenotoxic properties, and freeze-drying might be a useful processing method for blue mussels to retain their maximum physiological potential as a functional food.

키워드

참고문헌

  1. Ji LL. 1993. Antioxidant enzyme response to exercise and aging. Med Sci Sports Exerc 25: 225-231.
  2. Bokov A, Chaudhuri A, Richardson A. 2004. The role of oxidative damage and stress in aging. Mech Ageing Dev 125: 811-826. https://doi.org/10.1016/j.mad.2004.07.009
  3. Halliwell B. 1995. Antioxidant characterization: methodology and mechanism. Biochemical Pharmacology 49: 1341-1348. https://doi.org/10.1016/0006-2952(95)00088-H
  4. Kim MJ, Park E. 2012. Antioxidant and antigenotoxic effect of Schizandra chinensis B. extracted with water at various temperatures. J Cancer Prev 17: 128-140.
  5. Choi MJ, Kim MJ, Park EJ. 2012. Changes in the antioxidant activities and antigenotoxic effect of ethanol extracts of nine spring greens during short-term frozen storage after blanching. J Cancer Prev 17: 141-150.
  6. Ngo DH, Wijesekara I, Vo TS, Van Ta Q, Kim SK. 2011. Marine food-derived functional ingredients as potential antioxidants in the food industry: An overview. Food Res Int 44: 523-529. https://doi.org/10.1016/j.foodres.2010.12.030
  7. Jung ES, Park EJ, Park HR, Lee SC. 2008. Antioxidant activities of extracts from parts of Styela clava. J Korean Soc Food Sci Nutr 37: 1674-1678. https://doi.org/10.3746/jkfn.2008.37.12.1674
  8. Lee JH, Kim GH. 2013. Evaluation of antioxidant activity of marine algae-extracts from Korea. J Aquat Food Prod T doi: 10.1080/10498850.2013.770809.
  9. Lee SH, Shin JH, Koo MO, Jung ES, Jeon GI, Park EJ, Park HR, Lee SC. 2007. Antioxidant and antigenotoxic activities of extracts from anglerfish. J Korean Soc Food Sci Nutr 36: 1229-1234. https://doi.org/10.3746/jkfn.2007.36.10.1229
  10. Park JH, Seo BY, Lee SC, Park EJ. 2010. Effects of ethanol extracts from stalked sea squirt (Styela clava) on antioxidant potential, oxidative DNA damage and DNA repair. Food Sci Biotechnol 19: 1035-1040. https://doi.org/10.1007/s10068-010-0145-4
  11. Mamelona J, Pelletier E, Girard-Lalancette K, Legault J. 2007. Quantification of phenolic contents and antioxidant capacity of Atlantic sea cucumber, Cucumaria frondosa. Food Chem 104: 1040-1047. https://doi.org/10.1016/j.foodchem.2007.01.016
  12. Park SH. 2006. The interesting story of oceanic organism. Chusubat, Seoul, Korea. p 164-167.
  13. Choi WH. 1970. Studies on the variation in chemical constituents of the sea mussel, Mytilus edulis. Bull Korean Fish Soc 3: 38-44.
  14. Ministry of Oceans and Fisheries. 2013. Oceans and fisheries statistics annual report. Seoul, Korea. p 137.
  15. Cobb CS, Ernst E. 2006. Systematic review of a marine nutriceutical supplement in clinical trials for arthritis: the effectiveness of the New Zealand green-lipped mussel Perna canaliculus. Clin Rheumatol 25: 275-284. https://doi.org/10.1007/s10067-005-0001-8
  16. Coulson S, Vecchio P, Gramotnev H, Vitetta L. 2012. Greenlipped mussel (Perna canaliculatus) extract efficacy in knee osteoarthritis and improvement in gastrointestinal dysfunction: a pilot study. Inflammopharmacology 20: 71-76. https://doi.org/10.1007/s10787-012-0128-6
  17. Park JH, Kim SY, Kang MG, Yoon MS, Lee YI, Park EJ. 2012. Antioxidant activity and safety evaluation of juice containing Protaetia brevitarsis. J Korean Soc Food Sci Nutr 41: 41-48. https://doi.org/10.3746/jkfn.2012.41.1.041
  18. Lee EH, Ha JH, Cha YJ, Oh KS, Kwon CS. 1984. Preparation of powdered dried sea mussel and anchovy for instant soup. Bull Korean Fish Soc 17: 299-305.
  19. Kurihara H, Fukami H, Asami S, Totoda Y, Nakai M, Shibata H. 2004. Effects of oolong tea on plasma antioxidative capacity in mice loaded with restraint stress assessed using the oxygen radical absorbance capacity (ORAC) assay. Biol Pharm Bull 27: 1093-1098. https://doi.org/10.1248/bpb.27.1093
  20. Ou B, Hampsch-Woodill M, Prior RL. 2001. Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J Agric Food Chem 49: 4619-4626. https://doi.org/10.1021/jf010586o
  21. Lautraite S, Bigot-Lasserre D, Bars R, Carmichael N. 2003. Optimisation of cell-based assays for medium throughput screening of oxidative stress. Toxicol In Vitro 7: 207-220.
  22. Singh NP, McCoy MT, Tice RR, Schneider EL. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175: 184-191. https://doi.org/10.1016/0014-4827(88)90265-0
  23. Wang B, Li L, Chi CF, Ma JH, Luo HY, Xu YF. 2013. Purification and characterisation of a novel antioxidant peptide derived from blue mussel (Mytilus edulis) protein hydrolysate. Food Chem 138: 1713-1719. https://doi.org/10.1016/j.foodchem.2012.12.002
  24. Lee HJ, Kim MJ, Park JH, Park E. 2012. Antioxidative and antigenotoxic activity of white and yellow Chrysanthemum morifolium Ramat extracts. J Korean Soc Food Sci Nutr 41: 289-294. https://doi.org/10.3746/jkfn.2012.41.3.289
  25. Lee HR, Hwang IW, Ha HT, Chung SK. 2013. The antioxidant capacities of imported red wines (Cabernet Sauvignon) from US and Chile. Korean J Food Preserv 20: 608-613. https://doi.org/10.11002/kjfp.2013.20.5.608
  26. Seo BY, Park JH, Park HR, Lee SC, Park EJ. 2008. Antioxidant and antigenotoxic effect of various solvent extracts from tunic, substrate and whole part of Styela clava. Kyungnam Food and Biotechnology 7: 57-63.
  27. Kang DS, Ha BS. 1991. Carotenoid pigments of bivalves 1. Comparison of carotenoid pigments from muscle of mussel and blue mussel. J Korean Soc Food Nutr 20: 369-375.
  28. Partali V, Tangen K, Liaaen-Jensen S. 1989. Carotenoids in food chain studies - III. Resorption and metabolic transformation of carotenoids in Mytilus edulis (edible mussel). Comp Biochem Physiol B: Biochem Mol Biol 92: 239-246. https://doi.org/10.1016/0305-0491(89)90272-1
  29. Campbell SA. 1970. The carotenoid pigments of Mytilus edulis and Mytilus californianus. Comp Biochem Physiol 32: 97-115. https://doi.org/10.1016/0010-406X(70)90159-3
  30. Orak HH, Aktas T, Yagar H, Isbilir SS, Ekinci N, Sahin FH. 2012. Effects of hot air and freeze drying methods on antioxidant activity, colour and some nutritional characteristics of strawberry tree (Arbutus unedo L) fruit. Food Sci Technol Int 18: 391-402. https://doi.org/10.1177/1082013211428213
  31. Das A, Raychaudhuri U, Chakraborty R. 2012. Effect of freeze drying and oven drying on antioxidant properties of fresh wheatgrass. Int J Food Sci Nutr 63: 718-721. https://doi.org/10.3109/09637486.2011.644769
  32. Kumar SS, Manoj P, Shetty NP, Giridhar P. 2014. Effect of different drying methods on chlorophyll, ascorbic acid and antioxidant compounds retention of leaves of Hibiscus sabdariffa L. J Sci Food Agric doi: 10.1002/jsfa.6879.

피인용 문헌

  1. Antioxidant, ACE inhibitory, and acetylcholinesterase inhibitory activities of subcritical water extract of blue mussel vol.27, pp.3, 2018, https://doi.org/10.1007/s10068-018-0319-z