Journal of Applied Biological Chemistry

  • 제57권4호
  • /
  • Pages.301-305
  • /
  • 2014
  • /
  • 1976-0442(pISSN)
  • /
  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지
DOI QR코드

DOI QR Code

On-line Screening HPLC-ABTS+ assay를 이용한 청호로부터 유용성분의 항산화 활성 분석

Antioxidant Activity Analysis of Useful Compounds from Artemisiae Annuae Herba Using On-line Screening HPLC-ABTS+ Assay

  • Lee, Kwang Jin (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group) ;
  • Ma, Jin Yeul (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group)
  • 투고 : 2014.03.17
  • 심사 : 2014.05.13
  • 발행 : 2014.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

On-line screening high performance liquid chromatography (HPLC)-$ABTS^+$ assay를 이용한 청호로부터 chlorogenic acid, caffeic acid, vitexin, queretin, aremisinin의 항산화 활성을 온라인스크리닝 하였다. 이때 침적방법을 적용한 추출시간과 추출용매 조성으로부터 추출효율을 확인하였다. 이 결과 100% 물 추출물에서 수율이 가장 좋왔고 chloroenic acid의 항산화 활성이 가장 높았다. 또한 on-line screening HPLC-$ABTS^+$ assay 분석은 천연물에서 항산화 활성을 신속하게 탐색하는데 효율적이다.

The Antioxidant activity screening identification of five kind compounds in Artemisiae annuae herba with the on-line screening high performance liquid chromatography (HPLC) $ABTS^+$ assay. The various experimental variables such as the extraction time (h) and extraction solvent composition (%) of dipping method were investigated efficiently extraction at the room temperature $25^{\circ}C$. The results, the highest yield of total extract amount (0.458 g, 15.250%) was obtained by dipping method with 100% water and extraction time to 3 h. And the on-line screening HPLC-$ABTS^+$ assay method was rapid and efficient to search for bioactivity from natural products.

키워드

참고문헌

  1. Hazra B, Sarma MD, and Sanyal U (2004) Separation methods of quinonoid constituents of plants used in Oriental traditional medicines. J of Chroma B 812, 259-75. https://doi.org/10.1016/j.jchromb.2004.08.007
  2. Inoue K, Kitade M, Hino T, and Oka H (2011) Screening assay of angiotensin-converting enzyme inhibitory activity from complex natural colourants and foods using high-throughput LC-MS/MS. F Chemistry 126, 1909-15. https://doi.org/10.1016/j.foodchem.2010.12.019
  3. Jung HA, Nurul Islam MD, Kwon YS, Jin SE, Son YK, Park JJ et al. (2011) Extraction and identification of three major aldose reductase inhibitors from Artemisia montana. F and Chemical Toxi 49, 376-84. https://doi.org/10.1016/j.fct.2010.11.012
  4. Kim KS, Lee SH, Lee YS, Jung SH, Park YM, Shin KH et al. (2003) Antioxidant activities of the extracts from the herbs of Artemisia apiacea. J of Ethnoph 85, 69-72. https://doi.org/10.1016/S0378-8741(02)00338-0
  5. Kim KS, Lee SH, Shin JS, Shim SH, and Kim BK (2002) Arteminin, a new coumarin from Artemisia apiacea. Fitoterapia 73, 266-8. https://doi.org/10.1016/S0367-326X(02)00070-9
  6. Kim KS, Shim SH, Jang JM, Cheong JH, and Kim BK (1999) A Study on Hair-growth Activity of Artemisia apiacea Hance. Yakhak Hoeji 43, 798-01.
  7. Kim OC and Jang HJ (1994) Volatile components of Artemisia apiacea Herba. Agr Chemistry and Biot 37, 37-42.
  8. Lee KJ and Um BH (2008) Extraction of Useful component from Natural plants using Ultrasound system. Korean J Biotechnol Bioeng 23, 101-8.
  9. Lee KJ, Liang C, Yang HJ, and Ma JY (2012) Rapid Identification of Homoorientin from Phyllostachys bambusoides Leaves by HPLC On-line $ABTS^+$ Screening Method. Yakhak Hoeji 56, 217-21.
  10. Lee KJ, Shin YK, and Kim YS (2009) Enhanced effect extraction of Antioxidant substance Homoorientin from Pseudosasa japonica and Phyllostachys bambusoides leaves using Ultrasonic wave system. Korean J Biotechnol Bioeng 24, 189-94.
  11. Ridder SD, Kooy FVD, and Verpoorte R (2008) Artemisia annua as a self-reliant treatment for malaria in developing countries- Review. J of Ethnopharmacology 120, 302-14. https://doi.org/10.1016/j.jep.2008.09.017
  12. Song XY, Li YD, Shi YP, Ling J, and Chen J (2013) Quality control of traditional Chinese medicines: a review. Chinese J of Nat Medi 11, 596-607. https://doi.org/10.1016/S1875-5364(13)60069-2