Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation and Identification of Triterpenoids from the Mulberry (Morus alba) Root Bark

상백피(Morus alba root bark)로부터 triterpenoid의 분리 및 동정

  • Jung, Jae-Woo (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Park, Ji-Hae (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Jung, Ye-Jin (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Lee, Chang-Ho (Division of Matabolism and Functionality Research, Korea Food Research Institute) ;
  • Han, Daeseok (Division of Matabolism and Functionality Research, Korea Food Research Institute) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
  • Received : 2014.03.03
  • Accepted : 2014.05.08
  • Published : 2014.12.31
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Abstract

The mulberry (Morus alba L.) root barks were extracted with 80% aqueous methanol at room temperature. The concentrated extract was partitioned as ethyl acetate (EtOAc), n-BuOH, and $H_2O$ fractions. From the EtOAc fraction, five triterpenoids were isolated through the repeated silica gel and octadecyl $SiO_2$ column chromatographies. According to the results of physico-chemical and spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared, the chemical structures of the triterpenoids were respectively determined as ${\alpha}$-amyrin (1), ${\alpha}$-acetyl amyrin (2), lupeol (3), betulinic acid (4), and glutinol (5). Compounds 1, 3, and 5 were isolated for the first time from the mulberry root bark.

뽕나무(Morus alba L.) 뿌리껍질을 실온에서 80% MeOH 수용액으로 추출하고 이 추출물을 EtOAC 분획, n-BuOH 분획, $H_2O$ 분획으로 나누었다. EtOAc 분획에 대하여 silica gel과 ODS column chromatography를 반복 실시하여 5종의 triterpenoid를 분리, 정제하였다. NMR, IR, 및 EI/MS 등을 해석하여, ${\alpha}$-amyrin (1), ${\alpha}$-acetyl amyrin (2), lupeol (3), betulinic acid (4) 그리고 glutinol (5)로 구조동정 하였다. 화합물 중 a-amyrin (1), lupeol (3) 그리고 glutinol (5)은 상백피로부터는 이번 실험에서 처음으로 분리되었다.

Keywords

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