보존과학회지 (Journal of Conservation Science)

  • 제30권4호
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  • Pages.409-416
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  • 2014
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  • 1225-5459(pISSN)
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  • 2287-9781(eISSN)
한국문화재보존과학회 (The Korean Society Of Conservation Science For Cultural Heritage)
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김포 장기동 유적 출토 인골의 유전자 분석 연구

The Genetic Analysis Study of Ancient Human Bones Excavated at Janggi-dong site, Gimpo

  • 서민석 (국립문화재연구소 보존과학연구실) ;
  • 조은민 (국립문화재연구소 보존과학연구실) ;
  • 김윤지 (국립문화재연구소 보존과학연구실) ;
  • 김수훈 (국립문화재연구소 보존과학연구실) ;
  • 강소영 (국립문화재연구소 보존과학연구실)
  • Seo, Min Seok (Conservation Science Division, National Research Institute of Cultural Heritage) ;
  • Cho, Eun Min (Conservation Science Division, National Research Institute of Cultural Heritage) ;
  • Kim, Yun Ji (Conservation Science Division, National Research Institute of Cultural Heritage) ;
  • Kim, Sue Hoon (Conservation Science Division, National Research Institute of Cultural Heritage) ;
  • Kang, So Yeong (Conservation Science Division, National Research Institute of Cultural Heritage)
  • 투고 : 2014.10.04
  • 심사 : 2014.11.13
  • 발행 : 2014.12.20
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초록

유적지에서 발굴되는 조선시대 인골은 보존 상태가 대부분 양호하여 형질인류학, 유전학, 그리고 화학적 연구를 수행할 수 있다. 본 연구에서는 김포 장기동 유적지에서 발굴된 조선시대 인골 6개체에 대한 DNA 분석을 통하여 미토콘드리아 DNA 변이형을 결정하였으며, 성별 분석결과를 토대로 남성 피장자의 Y 하플로그룹을 동정하였다. 유전자 분석에 앞서 보존상태가 양호한 것으로 판단되는 인골 6구를 대상으로 조직학 지수를 판별하였다. 미토콘드리아 DNA 변이형은 아시아인에서 높은 빈도로 나타나는 G, R11, M7, A5 등의 하플로그룹이 확인되었다. 인골의 성별을 확인하기 위하여 아밀로제닌 유전자 분석을 수행한 결과, 6구의 인골에서 여성 4구와 남성 2구의 성별을 확인할 수 있었다. 남성으로 판명된 인골에 Y 염색체 단일염기다형성 분석을 적용해본 결과, 하플로그룹 O로 확인되었다. 향후 출토 인골의 광범위한 분석을 위하여 인골을 선별한 경우에는 본 연구에서 제시한 조직학 지수를 적용하고, 미토콘드리아 DNA와 핵 DNA의 다양한 분석을 병행하여 출토 인골간의 유연관계를 규명하는 것이 필요하다.

Most human bones of Joseon Dynasty period are so good condition that we can do research in physical anthropology, genetics and chemistry with them. In this study, we analyzed DNA typing using 6 human bones of Joseon dynasty period excavated at Janggi-dong, Gimpo. The DNA typing was mitochondrial DNA haplotype, Y-chromosome haplotype and sex determination. Prior to DNA analysis, we distinguished histological index of 6 human bones. As the result of mitochondrial DNA analysis, most of bones were confirmed as haplogroup G, R11, M7, A5, etc. As the result of sex determination, 4 human bones were female and 2 human bones were male. The male haplogroup was confirmed as haplogroup O by the single nucleotide polymorphism analysis of Y chromosome. For extensive ancient human bone analysis, researchers need to apply a histological index to select ancient human bones and explain a relationship among ancient human bones with various analyses of mitochondrial and nuclear DNA.

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피인용 문헌

  1. Principles of Archaeogenetics and the Current Trends of Ancient Genome Studies vol.31, pp.4, 2018, https://doi.org/10.11637/kjpa.2018.31.4.105