Advances in environmental research

Techno-Press (테크노프레스)
권호 표지
DOI QR코드

DOI QR Code

Optimization of Cu, Hg and Cd removal by Enterobacter cloacae by ferric ammonium citrate precipitation

  • Singh, Rashmi R. (Department of Microbiology and Biotechnology, School of Sciences, Gujarat University) ;
  • Tipre, Devayani R. (Department of Microbiology and Biotechnology, School of Sciences, Gujarat University) ;
  • Dave, Shailesh R. (Department of Microbiology and Biotechnology, School of Sciences, Gujarat University)
  • Received : 2014.01.17
  • Accepted : 2014.08.01
  • Published : 2014.12.25
⟨ Previous Next ⟩

Abstract

Iron precipitating organisms play a significant role in the formation of ferric hydroxide precipitate, which acts as strong adsorbent for toxic metal. In this respect four different iron precipitating cultures were isolated from Hutti gold mine surface winze water sample on citrate agar medium. The best isolate was screened out for metal removal study on the basis of fast visual iron precipitation. The selected isolate was identified as Enterobacter sp. based on routine biochemical tests and Biolog GN microplate results and as Enterobacter cloacae subsp. dissolvens by 16S rRNA gene sequence analysis (GenBank accession number EU429448). Influence of medium composition, medium initial pH, the influence of inoculum size, effect of various media and ferric ammonium citrate concentration were studied on metal removal in shake flask experiments. Under the optimized conditions studied, E. cloacae showed $94{\pm}2$, $95{\pm}2$ and $70{\pm}2%$ of cadmium, copper and mercury removal from a simulated waste in shake flask studies. In lab scale column reactor more than 85% of copper and mercury removal was achieved.

Keywords

References

  1. Staley, J.T., Bryant, M.P., Pfennig, N. and Holt, J.G. (1989), Bergey's Manual of Systematic Bacteriology, (1st Edition), Williams and Wilkins, Baltimore, MD, USA, Volume 3, pp. 1807-2008.
  2. Biolog (2001), Instructions for Use of the Biolog GP2 and GN2 Microplates, Biolog Inc., Hayword, CA, USA.
  3. Chakravorty, R. and van Grieken, R. (1986), "Radiochemical study of Cd, Co and Eu coprecipitation with iron hydroxide in seawater", Mikrochimica Acta, 90(1-2), 81-87. https://doi.org/10.1007/BF01196822
  4. Cullimore, D.R. and McCann, A.E. (1978), "The identification, cultivation and control of iron bacteria in ground water", Aquatic Microbiology, (Skinner, F.A. and Shewan, J.M. Eds.), Academic Press, London, UK, pp. 219-250.
  5. Dave, S.R. (2008), "Microbial interactions with inorganic pollutants: acid mine drainage, microbial accumulation of heavy metals and radionuclides", Appl. Microbiol., niscair India, 1-25. URL: http://nsdl.niscair.res.in/bitstream/123456789/646/1/MicrobialInteractions.pdf
  6. Eaton, A.D., Clesceri, L.S., Greenberg, A.E. and Franson, M.A.H. (1995), Standard Methods for the Examination of Water and Wastewater American Public Health Association, (19th Edition), Washington D.C., USA.
  7. Fujita, T., Dodbiba, G., Sadaki, J. and Shibayama, A. (2006), "Removal of anionic metal ions from wastewater by hydroxide-type adsorbents", The Chinese J. Proc. Eng., 6(3), 357-362.
  8. Hakeem, A.S. and Bhatnagar, S. (2010), "Heavy metal reduction of pulp and paper mill effluent by indigenous microbes", Asian J. Exp. Biol. Sci., 1(1), 201-203.
  9. Ibrahim, S.C., Hanafiah, M.A.K.M. and Yahya, M.Z.A. (2006), "Removal of cadmium from aqueous solution by adsorption onto sugarcane bagasse", American-Eurasian J. Agric. Environ. Sci., 1(3), 179-184.
  10. Jencarova, J. and Luptakova, A. (2012), "The elimination of heavy metal ions from waters by biogenic iron sulphides", Chem. Eng. Trans., 28, 205-210.
  11. Kumar, M. and Puri, A. (2012), "A review of permissible limits of drinking water", Indian J. Occup.Environ. Med., 16(1), 40-44. https://doi.org/10.4103/0019-5278.99696
  12. Leake, T.R. (2009), "Zinc removal using biogenic iron oxides", M.Sc. Thesis, Washington State University, Pullman, WA, USA.
  13. Lee, A.J. and Lee, G.F. (2005), "Role of iron chemistry in controlling the release of pollutants from resuspended sediments", Remediation J., 16(1), 33-41. https://doi.org/10.1002/rem.20068
  14. Mortazavi, S., Rezaee, A., Khavanin, A., Varmazyar, S. and Jafarzadeh, M. (2005), "Removal of mercuric chloride by a mercury resistant Pseudomonas putida strain", J. Biol. Sci., 5(3), 269-273. https://doi.org/10.3923/jbs.2005.269.273
  15. Okoronkwo, N.E., Igwe, J.C. and Okoronkwo, I.J. (2007), "Environmental impacts of mercury and its detoxification from aqueous solutions", African J. Biotechnol., 6(4), 335-340.
  16. Olguin, E.J. and Sanchez-Galvan, G. (2012), "Heavy metal removal in phytofiltration and phycoremediation: The need to differentiate between bioadsorption and bioaccumulation", New Biotechnol., 30(1), 3-8. https://doi.org/10.1016/j.nbt.2012.05.020
  17. Rajasimman, M. and Murugaiyan, K. (2010), "Optimization of process variables for the biosorption of chromium using Hypne, valentiae", Nova Biotechnologica, 10(2), 107-115.
  18. Rajeshkumar, R. and Kartic, N. (2011), "Removal of $Cu^{2+}$ ions from aqueous solutions using copper resistant bacteria", Our Nature 9, 49-54.
  19. Reddy, K.J., Wang, L. and Gloss, S.P. (1995), "Solubility and mobility of copper, zinc and lead in acidic environments", Plant and Soil, 64, 141-146.
  20. Safavi, K., Asgari, M.J. and Padidar, M. (2011), "The effect of microorganisms on soil remediation", Proceedings of 2010 International Conference on Biology, Environment and Chemistry, IACSIT Press, Hong Kong, December, Volume 1, pp. 359-363.
  21. Samarth, D.P., Chandekar, C.J. and Bhadekar, R.K. (2012), "Biosorption of heavy metals from aqueous solution using Bacillus licheniformis", Int. J. Pure Appl. Sci. Technol., 10(2), 12-19.
  22. Shetty, R. and Rajkumar, S. (2009), "Biosorption of $Cu^{2+}$ by metal resistant Pseudomonas sp.", Int. J. Environ. Res., 3(1), 121-128.
  23. Sinha, A. and Khare, S.K. (2012), "Mercury bioremediation by mercury accumulating Enterobacter sp. cells and its alginate immobilized application", Biodegradation, 23(1), 25-34. https://doi.org/10.1007/s10532-011-9483-z
  24. Solisio, C., Lodi, A., Converti, A. and Borghi, M. (2000), "The effect of acid pre-treatment on the biosorption of chromium (III) by Sphaerotilus natans from industrial wastewater", Wat. Res., 34(12), 3171-3178. https://doi.org/10.1016/S0043-1354(00)00059-2
  25. Tansupo, P., Budzikiewicz, H., Chanthai, S. and Ruangviriyachai, C. (2008), "Effect of pH on the mobilization of copper and iron by pyoverdin I in an artificially contaminated soils", Sci. Asia, 34, 287-292. https://doi.org/10.2306/scienceasia1513-1874.2008.34.287
  26. Tilaki, D. and Ali, R. (2003), "Study on removal of cadmium from water environment by adsorption on GAC, BAC and biofilter", Pakistan J. Biol. Sci., 7(5), 865-869.
  27. Vogel, A.I. (1961), Quantitative Inorganic Analysis, Longmans Green and Co. Ltd., London, UK.
  28. Volesky, B. (1986), "Biosorbent materials", Biotechnology and Bioengineering Symposium, John Wiley & Sons, New York, NY, USA, No. 16, pp. 121-126.
  29. Volesky, B. (2007), "Biosorption and me", Water Res., 41(18), 4017-4029. https://doi.org/10.1016/j.watres.2007.05.062