Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Analysis of Inflammatory Cytokines from the Cecum and Proximal Colon of Mice Infected with Enterotoxigenic Bacteroides fragilis

  • Hwang, Soonjae (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju) ;
  • Lee, Min Ho (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju) ;
  • Gwon, Sun-Yeong (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju) ;
  • Lee, Seunghyung (Department of Anatomy & Cell Biology, Temple University School of Medicine) ;
  • Jung, Dongju (Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University) ;
  • Rhee, Ki-Jong (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju)
  • Received : 2013.05.14
  • Accepted : 2013.06.22
  • Published : 2013.06.29
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Abstract

Enterotoxigenic Bacteroides fragilis (ETBF) causes inflammatory diarrhea in humans and animals and is also implicated in colorectal cancer. ETBF-infected mice exhibit a prominent large intestinal inflammation characterized by neutrophil infiltration and induction of the Th17 response. In this study, we examined differences in the secreted cytokine profile of the cecum and proximal colon of ETBF-infected mice using an antibody array. Of the cytokines examined, we found that the cecal tissues from ETBF-infected mice secreted elevated levels of G-CSF, IL-6, IL-17 and LIX compared to non-toxigenic Bacteroides fragilis (NTBF) and Mock infected mice. The proximal colon tissues from ETBF-infected mice secreted higher levels of G-CSF, IL-6, KC, LIX, MIP-1g and MCP-1. This study demonstrates that the cecum and colon should be considered separately when assays are used to determine immune responsiveness to enteric infections.

Keywords

References

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