대한의생명과학회지 (Biomedical Science Letters)

  • 제19권2호
  • /
  • Pages.132-141
  • /
  • 2013
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
권호 표지

Distribution and Antibiotic Susceptibility Patterns of Genus Mycobacterium at a Private Hospital, Korea

  • Hong, Sung Kyun (Department of Clinical Laboratory Science, Sanggae Paik Hospital, Inje University) ;
  • Hur, Sung-Ho (Department of Medical Laboratory Science, Dong-Eui Institute of Techonology) ;
  • Seong, Hee-Kyung (Department of Medical Laboratory Science, Dong-Eui Institute of Techonology)
  • 투고 : 2013.02.15
  • 심사 : 2013.05.06
  • 발행 : 2013.06.29
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초록

Mycobacterium isolates were retrospectively identified, antibiotics susceptibility test results and basic clinical data were analyzed for the 715, excepted 308 in 1,023 specimens, from a mycobacterial laboratory at a tertiary care hospital from September 2002 to December 2008. Their male to female ratio was 1.12 to 1 (379 male, 336 female). The median age of study population was 47 years (range from 10 to 93 years). Distribution of Mycobacterium species was 90.1% of total were isolates Mycobacterium tuberculosis, and 9.9% of the total non-tuberculosis Mycobacterium isolated, and Among nontuberculosis Mycobacterium isolates, 60.6% were Mycobacterium avium complex, 14.1% were isolates Mycobacterium abscessus, and 12.7% were isolates Mycobacterium intracellulare. Among 526 Mycobacterium tuberculosis isolates, 81.7% isolates were susceptible to first line antibiotics, 18.3% were resistant to one or more antibiotics. Non-tuberculosis Mycobacterium isolates, all were resistant to two or more antibiotics. Multi-antibiotic resistant tuberculosis rate was show 10.2% of total specimens. Isolated Mycobacterium species, 19.2% were multi-antibiotic resistant tuberculosis, and the rate of nontuberculosis Mycobacterium resistant to isoniazid and rifampin was very highly 84.5%. Thus among acid fast bacilli culture positive cases, Mycobacterium tuberculosis and non-tuberculosis Mycobacterium were must exactly identification and antibiotic sensitivity test. It was considered to help to select of the antibiotic in preventive medicine.

키워드

참고문헌

  1. American Thoracic Society. Diagnostic standards and classification of tuberculosis in adults and children. Am J Respir Crit Care Med. 2000. 161: 1376-1395. https://doi.org/10.1164/ajrccm.161.4.16141
  2. Anargyros P, Astill DS, Lim IS. Comparison of improved BACTEC and Lowenstein-Jensen media for culture of mycobacteria from clinical specimens. J Clin Microbiol. 1990. 28: 1288-1291.
  3. Averg JA, Yajko DM, Jacobson MA. Eradication of AIDS-related disseminated Mycobacterium avium complex infection after 12 months of antimycobacterial therapy combined with highly active antiretroviral therapy. J Infect Dis. 1998. 178: 1446-1449. https://doi.org/10.1086/314469
  4. Bai GH, Park KS, Kim SJ. Clinically isolated mycobacteria other than Mycobacterium tuberculosis from 1980 to 1990 in Korea. J Korean Microbiol. 1993. 28: 1-5.
  5. Canetti G, Fox W, Khomenko A, Mahler HT, Menon NK, Mitchison DA, Rist N, Smelev NA. Advances in techniques of testing mycobacterial drug sensitivity and the use of sensitivity tests in tuberculosis control programmes. Bull World Health Organ. 1969. 41: 21-43.
  6. Centers for disease Control and Prevention. Use of fluorochrome staining for detecting acid-fast Mycobacteria, 2000. Department of health and human services. Centers for disease control and prevention. Atlanta, GA. 2000. 1-17.
  7. Centers for disease Control and Prevention. Update: Nucleic acid amplification tests for tuberculosis. MMWR 2000. 49: 593-602.
  8. Centers for disease Control and Prevention. Updated guideline for Nucleic acid amplification tests in the diagnosis of tuberculosis. MMWR 2009. 58: 7-10.
  9. Chang CH, Park TS, Kim MN, Lee NY, Lee HJ, Suh JT. Survey on Changes in Mycobacterial Testing Practicies in Korean Laboratories. Korean J Clin Microbiol. 2001. 4: 108-114.
  10. Choi SH, Sun WS, Kim MN, Shim TS. The clinical characters, diagnosis, treatment, and outcomes of patients with tuberculosis at a private university hospital in Korea. J Korean Acad Fam Med. 2005. 26: 481-489.
  11. Clark G. Staining procedure. 1973. p225. William and Wilkins Co., Baltimore. 3rd ed.
  12. Clinical and Laboratory Standard Institute (CLSI). Laboratory Detection and Identification of Mycobacteria. Approved Guideline, CLSI document M48-A. Wayne PA. 2008.
  13. Devallois A, Goh KS, Rastogi N. Rapid identification of mycobacteria to species level by PCR-restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species. J Clin Microbiol. 1997. 35: 2969-2973.
  14. Foo H, van Hal S, Jelfs P, Gilbert GL. Antimicrobial resistance in non-tuberculous mycobacteria in New South Wales, 2002-2008. Int J Antimicrob Agents. 2009. 34: 182-184. https://doi.org/10.1016/j.ijantimicag.2009.01.013
  15. Gail LW, Barbara ABE, Edward PD, Geraldine SH, Leonid H, Gaby EP, John CR, Richard JW, Nancy GW, Frank GW. Susceptibility Testing of Mycobacteria, Nocardiae, and Other Aerobic Actinomycetes. Approved Standard, M24-A2E. 2011. Clinical and Laboratory Standards Institute. 2011. p76.
  16. Gordin F, Slutkin G. The validity of acid-fast smears in the diagnosis of pulmonary tuberculosis. Arch Pathol Lab Med. 1990. 114: 1025-1027.
  17. Griffith, DE, Aksamit T, Brown-Elliott BA, Catanzaro A, Daley C, Gordin F, Holland SM, Horsburgh R, Huitt G, Iademarco MF, Iseman M, Olivier K, Ruoss S, von Reyn CF, Wallace RJ, Winthrop K. An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med. 2007. 175: 367-416. https://doi.org/10.1164/rccm.200604-571ST
  18. Haverkort F. National atypical mycobacteria survey, 2000. Commun Dis Intell. 2003. 27: 180-189.
  19. Heifets L. Qualitative and quantitative drug-susceptibility tests in mycobacteriology. Am Rev Respir Dis. 1988. 137: 1217-1222. https://doi.org/10.1164/ajrccm/137.5.1217
  20. Hong YP, Kim SJ, Kwon DW, Chang SC, Lew WJ, Han YC. The sixth nationwide tuberculosis prevalence survey in Korea, 1990. Int J Tubercule Lung Dis. 1993. 74: 323-331. https://doi.org/10.1016/0962-8479(93)90107-9
  21. Hong YP, Kim SJ, Lew WJ, Lee FK, Han YC. The seventh nationwide tuberculosis prevalence survey in Korea, 1995. Int J Tubercule Lung Dis. 1998. 2: 27-36.
  22. Ji YY, Kim JP, Shin JH, Suh SP, Ryang DW. Detection of Mycobacterium tuberculosis using BACTEC mycobacteria growth indicator tube (MGIT) 960 system-comparison with BACTEC 460 TB system and Ogawa media. Korean J Lab Med. 2000. 20: 384-391.
  23. Jun HJ, Jeon KN, Um SW, Kwon OJ, Lee NY, Koh WJ. Nontuberculous mycobacteria isolated during the treatment of pulmonary tuberculosis. Respiratory Medicine. 2009. 103: 1936-1940. https://doi.org/10.1016/j.rmed.2009.05.025
  24. Jung GW, Jeong KO, Min YC, Man EG, Yeong SJ, Pyo JM, Jung KH, Han SW, Park SY, Lee NY. Recovery rate of nontuberculous mycobacteria from acid-fast-bacilli smear-positive sputum specimens. Tuber Respir Dis. 2003. 54: 22-32. https://doi.org/10.4046/trd.2003.54.1.22
  25. Kent PT, Kubica GP. Public health mycobacteriology: a Guide for the level III laboratory. 1985. 21: 654-668. Center for Disease Control, Atlanta.
  26. Kevy H, Feldman C, Sacho H, van der Meulen H, Kallenbach J, Koornhof H. A reevaluation of sputum microscopy and culture in the diagnosis of pulmonary tuberculosis. Chest. 1989. 95: 1193-1197. https://doi.org/10.1378/chest.95.6.1193
  27. Kim HJ, Oh H, Lee WY, Kim SJ, Case Report: Report of a case of pulmonary mycobacteriosis caused by Mycobacterium chelonei subsp. abscessus. Tuber Respir Dis. 1985. 32: 54-57.
  28. Kim MN, Lee SH, Yang SE, Pai CH. Mycobacterial testing in hospital laboratories in Korea: Results of a survey of 40 university or tertiary-care hospitals. Korean J Clin Pathol. 1999. 19: 86-91.
  29. Kim SJ. Drug Resistance of Mycobacterium tuberculosis in Korea. Korean Chemothra. 1993. 11: 34-39.
  30. Koh HK, Kang YJ, Lim SY, Shin JW, Choi JS, Yoo JH, Kim JY, Park W, Choi BW, Hue SH. Predictors of drug-resistance in patients with pulmonary tuberculosis. Tuber Respir Dis. 1999. 46: 311-316. https://doi.org/10.4046/trd.1999.46.3.311
  31. Koh WJ, Kwon OJ, Yu CM. Recovery rate of nontuberculous mycobacteria from acid-fast-bacilli smear positive sputum specimens. Tuber Respir Dis. 2003. 54: 22-32. https://doi.org/10.4046/trd.2003.54.1.22
  32. Koh WJ, Kwon OJ, Jeon K, Kim TS, Lee KS, Park YK, Bai GH. Clinical significance of nontuberculous mycobacteria isolated from respiratory specimens in Korea. Chest. 2006. 129: 341-348. https://doi.org/10.1378/chest.129.2.341
  33. Korean Center for Disease Control and Prevention. Assessment of tuberculosis control work in Korea, 2003. Korean Center for Disease Control and Prevention. 2003.
  34. Korean Center for Disease Control and Prevention. Guidelines for the control of tuberculosis, 2004. Korean Center for Disease Control and Prevention. 2004.
  35. Kurashima A. Japanese new guidelines for nontuberculous mycobacterial pulmonary disease. Kekkaku. 2010. 85: 87-93.
  36. Lamden K, Watson JM, Knerer G, Ryan MJ, Jenkins PA. Opportunist mycobacteria in England and Wales: 1982 to 1994. Commun Dis Rep CDR Rev. 1996. 6: 147-151.
  37. Lee HW, Kim MN, Sim TS, Bae GH, Bae JH. Nontuberculous mycobacterial pulmonary infection in immunocompetent patients. Tuber Respir Dis. 2002. 53: 173-182. https://doi.org/10.4046/trd.2002.53.2.173
  38. Lew WJ, Ahn DI, Yoon YJ, Cho JS, Kwon DW, Kim SJ, Hong YP. Clinical Experience on Mycobacterial Diseases Other Than tuberculosis. Tuber Respir Dis. 1992. 39: 425-432.
  39. Lew WJ. Tuberculosis situation in Korea. Tuber Respir Dis. 1999. 46: 301-310. https://doi.org/10.4046/trd.1999.46.3.301
  40. Liu CH, Li HM, Li L, Hu YL, Wang Q, Yang N, Wang S, Zhu B. Anti-tuberculosis drug resistance patterns and trends in a tuberculosis referral hospital, 1997-2009. Epidemiol Infect. 2011. 139: 1909-1918. https://doi.org/10.1017/S0950268810003158
  41. Lopez AD, Murray CC. The Global Burden of Disease, 1990-2020. Nat. Med. 1998. 4: 1241-1243.
  42. Marras TK, Daley CL. Epidemiology of human pulmonary infection with nontuberculous mycobacteria. Clin Chest Med. 2002. 23: 553-567. https://doi.org/10.1016/S0272-5231(02)00019-9
  43. Matthaei E. Simplified Fluorescence Microscopy of Tubercle Bacilli. J Gen Microbiol. 1950. 4: 393-398. https://doi.org/10.1099/00221287-4-3-393
  44. Moore JE, Kruijshaar ME, Ormerod LP, Drobniewski F, Abubakar I. Increasing reports of non-tuberculous mycobacteria in England, Wales and Northern Ireland, 1995-2006. BMC Public Health. 2010. 10: 1471-2458.
  45. Nah J, Huh JW, Lee SH, Kim BC, Koh YS, Pai CH. Identification of Mycobacterium tuberculosis complex using a gene probe method. Korean J Lab Med. 1997. 17: 71-78.
  46. National Committee for Clinical Laboratory Standards. Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes; approved standard. Document M24-A. National Committee for Clinical Laboratory Standards, Wayne, PA. 2003. 23: 25-32.
  47. Nowak R. WHO calls for action against TB. Science. 1995. 267: 17-63.
  48. Ogawa T, Ueno T, Takakura S. A two-layer medium for quantitative culture of tubercle bacilli. First report: Experiments to establish a two-layer medium. Kekkaku. 1960. 35: 187-192.
  49. Park HS, Jhin DI, Jeong YS, Kwon OH. Effect of N-acetyl-L-cysteine decontamination on the contamination rate of medium and culture positive rate on Mycobacterium. Korean Qual Assur Clin Lab. 1991. 13: 223-228.
  50. Park S, Kim S, Park EM, Kim H, Kwon OJ, Chang CL, Lew WJ, Park YK, Koh WJ. In Vitro Antimicrobial Susceptibility of Mycobacterium abscessus in Korea. J Korean Med Sci. 2008. 23: 49-52.
  51. Robert GD, Koneman EW, Kim YK. Mycobacterium. In Balows A, Hausler WJ, Hermann KL, Isenberg HD, and Shadomy HJ, Manual of Clinical Microbilogy, 5th ed. American Society for Microbiology, Washington DC. 1991. 304-339.
  52. Schluger NW. Changing approaches to the diagnosis of tuberculosis. Am J Respir Crit Care Med. 2001. 164: 2020-2024. https://doi.org/10.1164/ajrccm.164.11.2008100
  53. Scientific Committee in Korean Academy of Tuberculosis and Respiratory Diseases. National survey of mycobacterial diseases other than tuberculosis in Korea. Tuberc Respir Dis. 1995. 42: 277-294. https://doi.org/10.4046/trd.1995.42.3.277
  54. Son CH, Yang DG, No MS, Jeong JS, Lee H, Lee GN, Choe PJ, Lee SG, Jang GY, Choe IS. Prevalence of drug-resistances in patients with pulmonary tuberculosis and its association with clinical characteristics at one tertiary R hospital in Pusan, Korea. Tuber Respir Dis. 2001. 51: 416-425. https://doi.org/10.4046/trd.2001.51.5.416
  55. Stone BL, Burman WJ, Hildred MV, Jarboe EA, Reves RR, Wilson ML. The diagnostic yield of acid-fast-bacillus smear-positive sputum specimens. J Clin Microbiol. 1997. 35: 1030-1031.
  56. Thomson RM. Changing epidemiology of pulmonary nontuberculous mycobacteria infections. Emerg Infect Dis. 2010. 16: 1576-1583. https://doi.org/10.3201/eid1610.091201
  57. Truant JP, Brett WA, Thomas W Jr. Fluorescence microscopy of tubercle bacilli stained with auramine and rhodamine. Henry Ford Hosp Med Bull. 1962. 10: 287-296.
  58. van Ingena J, van der Laanb T, Dekhuijzena R, Boeree M, van Soolingen D. In vitro drug susceptibility of 2275 clinical non-tuberculous Mycobacterium isolates of 49 species in The Netherlands. Int J Antimicrob Agents. 2010. 35: 169-173. https://doi.org/10.1016/j.ijantimicag.2009.09.023
  59. Wang HX, Yue J, Han M, Yang JH, Gao RL, Jing LJ, Yang SS, Zhao YL. Nontuberculous mycobacteria: susceptibility pattern and prevalence rate in Shanghai from 2005 to 2008. Chin Med J. 2010. 123: 184-187.
  60. WHO.The world health report 2006-Working together for health. World Health Organization. Genava. 2006. 169-177.
  61. Wood GL, Lipsky BA, Gates J, Tenover FC. Factors affecting the clinical value of microscopy for acid-fast bacilli. Rev Infect Dis. 1984. 6: 214-222. https://doi.org/10.1093/clinids/6.2.214
  62. Wright PW, Wallace RJ Jr, Wright NW, Brown BA, Griffith DE. Sensitivity of fluorochrome microscopy for detection of Mycobacterium tuberculosis versus nontuberculosis mycobacteria. J Clin. Microbiol. 1998. 36: 1046-1049.
  63. Yajko DM, Nassos PS, Sanders CA Madei JJ, Hadley WK. High predictive value of the acid-fast smear for Mycobacterium tuberculosis despite the high prevalence of Mycobacterium avium complex in respiratory specimens. Clin Infect Dis. 1994. 19: 334-336. https://doi.org/10.1093/clinids/19.2.334