대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)

  • 제27권4호
  • /
  • Pages.108-121
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  • 2013
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  • 1226-8038(pISSN)
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  • 2287-9463(eISSN)
대한한방소아과학회 (The Association of Korean Oriental Pediatrics)
권호 표지
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DOI QR Code

가미이진탕(加味二陳湯) 전탕액과 발효액이 항비만(抗肥滿)효과에 미치는 연구

The Study on Anti-obesity Effects of Gamiygin-tang Extract and Ferment

  • 장성진 (원광대학교 한의과대학 소아과교실) ;
  • 민들레 (원광대학교 한의과대학 소아과교실) ;
  • 박은정 (원광대학교 한의과대학 소아과교실)
  • Chang, Sung Jin (Department of Pediatrics, College of Oriental Medicine, Wonkwang University) ;
  • Min, Deul Le (Department of Pediatrics, College of Oriental Medicine, Wonkwang University) ;
  • Park, Eun Jung (Department of Pediatrics, College of Oriental Medicine, Wonkwang University)
  • 투고 : 2013.10.25
  • 심사 : 2013.11.14
  • 발행 : 2013.11.30
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초록

Objective This study was designed to investigate the effects of Gamiygin-tang (GY) extract (GYE) and fermented solution (GYF) on body weight, serum lipid level and adipocyte differentiation in high fat diet-fed obese mice. Materials and Methods High fat diet-fed obese mice and 3T3-L1 adipocytes mice were treated with GYE and GYF and obesity related markers were assessed. A cytotoxicity assay was carried out by MTS assay. Inhibitory effects of GYE and GYF on adipocyte differentiation were carried out by Oil Red O staining. The effects of GYE and GYF on the expression of adipocyte differentiation regulatory factors, peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) and CCAAT/enhancer binding protein alpha (CEBP-${\alpha}$) were measured by real-time reverse transcriptase-polymerase chain reaction. The effects of GYE and GYF on the expression of adipocyte differentiation regulatory factors were also determined in relation to protein production/protein levels by western blotting. The anti obesity effects of GYE and GYF were measured in high fat-diet induced obese mice. Various factors were measured from the serum of the high fat-diet mice. Results Though GYE did not show toxicity at the concentration of 1mg/ml, GYF showed toxicity at the concentration of 1mg/ml. The GYE at 0.1 and 1mg/ml inhibited the differentiation of 3T3-L1 cells, and the GYF also inhibited the differentiation of 3T3-L1 cells. The effect of GYE on adipocyte differentiation factors including PPAR-${\gamma}$ and CEBP-${\alpha}$ was investigated and compared to the corresponding concentration levels of GYF. GYE and GYF both suppressed the RNA and protein levels of adipocyte differentiation factors. In the animal test both GYE and GYF reduced weight gain. GYE and GYF reduced blood cholesterol, TG and LDL levels. GYF better reduced blood cholesterol levels. Conclusions These results demonstrate that GYE and GYF exerts anti-obesity effect in 3T3-L1 cells and obese mice induced by high-fat diet.

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참고문헌

  1. Kim YS. Definition and diagnosis classification of obesity. The Journal of Korean Medical Association. 1994;37(9): 1108-14.
  2. WHO. "Obesity Prevention and the Global Epidemic-Report of WHO Consolation on Obesity". 1997.
  3. Korea Health Industry Development Institute(KHIDI). Public Health Nutrition Survey. Seoul:KHIDI. 2005: 38-44.
  4. National Health Insurance Service Autumn Issue. National Health Insurance Service. 2008.
  5. Wang Bing Bian Zhu. Huangdi Neijing Su Wen Publishing Co:Dae Sung Publishing Co. 1994:200, 287.
  6. Ko SC, Jung KM. East-West Medical Study on Infantile Obesity. Journal Pediatrics of Korean Medicine. 1989; 3(1):57-61.
  7. Zhang Jie Bin. Jing Yue Quan Shu. Seoul:Dae Sung Publishing Co. 1992:211-2.
  8. Liu Jing Yuan Dian Xiao. Taiping Huimin He Ji Ju Bian. Taiping Huimin He Ji Ju Fang. Beijing: Ren Min Wei Sheng Publishing Co. 1983:141.
  9. Yun YG. Eastern Medicine Prescriptions and Analysis. Seoul: Eui Sung Dang Publishing Co. 1998:233-4.
  10. Yun YG. Prescriptional Study on the Application of Ijintang. The Korean Journal of Oriental Medical Prescription. 1999;7(1):11-35.
  11. Chuan Jing Zhu. Chuan Jing Zhu Nan Ke[Shang]. Kyughee University HanJuk Library. 고619.3부52ㅂ(300119859).
  12. Chen Shi Duo, Translated by No YK. Ben Cao Bi Lu Bian Zheng Yu Han. Mun won Publishing Co. 2005:691.
  13. Shin DW. Korean Medicine Sermon II. Shinilbooks Publishing Co. 2010:327.
  14. Kim KY, Song HJ . Korean Herbology Drug Processing. Seoul:Shinilbooks Publishing Co. 2002:547.
  15. Liang C, Yun NY, Jung SW, Kim DS, Lee YJ, Ma JY. Analysis of the Components of Guibitang and Fermented Guibi-tang and their Ability to Inhibit Angiotensin-converting Enzyme. Natural Product Sciences. 2011;17(4):363-6.
  16. Kim DS, Um YR, Yang YR, Yun NY, Ma JY. PolyphenolContents and Antioxidant Activities of Fractions from Ssanhwa-tang and Fermented SSanghwatang. Korean Journal of Oriental Medicine. 2010;16(3): 175-8.
  17. Han JH, LEE SY. Comparing Medical Efficacy of Socheongyong-tang with Lactic Acid Bacteria Fermented Socheongyong-tang. Korean journal of Oriental Physiology and Pathology.
  18. Lee SJ, Lee MJ, Joeng JE, Kim HJ. Antimicrobial Effects of Fermented Coptidis rhizoma and Lonicerae Flos against pathogen. Journal of Korean Oriental Association for Study of Obesty. 2011;11(1): 35-46.
  19. Kim EH, Kim KS, Chae SK, Kim BS, Kang JS. Comparison of Biological Activities on Rehmanniae Radix and Fermented Rehmanniae Radix. Korean Journal of Oriental Physiology and Pathology. 2012;26(3):306-13.
  20. Choi JE, Cho JH, Jang JB, Lee KS. The effects of Yi-jin on Body Weight and Ovarian Reaction in Obesity Mice. The Journal of Oriental Obstetrics & Gynecology. 2003;16(2):68-75.
  21. Oh JH, Kim BS, Lim HY, Kim DW, Choi BH, Hur JI, Kim DJ, Cho CK, Byun JS. Three Cases Report of Functional Dyspepsia Patients Who were Administered by LJTG(Ljintang-Gamibang(二陳湯 加味方). The journal of Korean Oriental Internal Medicine. 2005;26(3): 641-51.
  22. Kim DJ. Single Oral Dose Toxicity Test of Iijintanggami-bang a Polyherbal Formula in ICR Mice. Korean Journal of Oriental Physiology and Pathology. 2010;24(6):1019-26.
  23. Ok MJ, Byun JS, Park SD, Lee HI. Effect of Yijintang(Erchen-tang) and Gamiyijin-tang(Jiaweierchen-tang) on the Gastrointestinal Functions of the Rats. The journal of Korean Oriental Medicine. 2002;23(3):11-25.
  24. The Korean Society for Oriental Rehabilitation Medicine. Oriental Rehabilitation Medicine. Seoul:Kun Ja Publishing Co. 2005:349-62, 384-96.
  25. Jung SH. A Study on Methods to Obtain Obesity Related Information. Journal of Korean Medicine Rehabilitation. 1998;8(2):1-15.
  26. Department of Rehabilitation Medicine, National Oriental Medicine Universities. Korean Medicine Rehabilitation. Seoul:Seo Won Publishing Co.1995:570-6.
  27. Lee GY, Park TJ. The Influence of Obesity on Health in Adults at or over forty years. Korean J Fam Med. 1997;18(3):284-94.
  28. Song MY, Jeong WS, Shin HD. Clinical Trial of Herbal fomula(slim-diet) on Weight Loss in Obese Premenopausal Korean Females. Journal of Korean Oriental Association for Study of Obesity. 2003;3(1):1-6.
  29. Go SC, Jeong KM. East-West Medical Study on Infantile Obesity. The Journal of Korean Oriental Pediatrics. 1989;3(1):57-61.
  30. Cha YY. A Clinical Study for the Influence of Bigiheo Herbal Acupuncture Therapy on Abdominal Obesity. Journal of Korean Oriental Association for Study of Obesity. 2004;4(1):61-5.
  31. Shin HD, Kim SS, Lee ES. A Comparative Study on Clinical Treatment of Obesity. The Journal of Korean Medical Association. 1992;13(2):63.
  32. Department of Acupuncture and Moxibustion, National Oriental Medicine Universities. Acupuncture and Moxibustion. Seoul:Jib Moon Dang Publishing Co. 1988.
  33. Kim KO. Medical Qigong II. Seoul:Dan Bi Publishing Co. 1994:68.
  34. Oh JH. Manual Acupressure. Seoul:Han Sung Sa Publishing Co. 1990:331-2.
  35. Ki JS. Eastern Medicine Negative Cupping Treatment. Seoul:Tae Woong Publishing Co. 1993:145.
  36. Kim SM , Kim GS. Effect of Chegamuiyin-tang and Electro-lipolysis Acupuncture on the Reduction of Body Fat(Effect of Oriental Medicine on Localized Obesity). Journal of Korean Oriental Association for Study of Obesity. 2002;2(1):12-23.
  37. Korean Oriental Association for Study of Obesity. Clinical Obesity Treatment. Seoul:Korea Medicine Publishing Co. 2001.
  38. Park YW. Evidence Based Formulas Composed of Health/Functional Foods to Control Weight. Korean J Fam Med. 2003;24(5):409-15.
  39. Park CY. The Update of Anti-Obesity Drug. Kyung Hee Medicine Publishing Co. 2002;18(2):77-85.
  40. Jin KS. Analysis of Obesity Research Trends. Dajeon University Graduate School. 2004.
  41. Choi JS, Oh MS. Inhibitory Effects of Bogigambi-tang on the Obese-mouse Induced by High Fat Diet. Korean Journal of Oriental Physiology and Pathology. 2007; 21(3):634-41.
  42. Park JS, Lee BC, Doo HK, Ahn YM, Ahn SY. The Effects of Supungsunki-hwan on High Fat, High Carbohydrate Diet-induced Obese Type 2 Diabetic Mouse Model. The Journal of Korean Oriental Internal Medicine. 2009;30(2):257-69.
  43. Park TY, Shin BC, Gong JC, Song MY, Kim EK, Seo EA, Ryu DG, Kwon KB. Study on Anti-obesity Effect of Chegameuiin-tang. Korean Journal of Oriental Physiology and Pathology. 2008;22(3):642-8.
  44. Ban SS, Yoon HD, Shin OC, Shin YJ, Park JS, Park JH, Seo BI. The Effects of Artemisiae Capillaris, Ponciri Fructus and Cataegi Fructus in Obese Rats Induced by High Fat Diet. The Korea Journal of Herbology. 2006;21(3):55-67.
  45. Shin YJ, Kim HJ, Lee MJ, Keum DH. The Effect of Fermented Ephedrae Herba on Rats Fed High Diet. Journal of Korean Medicine Rehabilitation. 2009;19(4):37-57.
  46. Shin YJ, Kim HJ, Lee MJ. The Effect of Pinus densiflora on induced by High Fat Diets in Rats. Journal of Korean Medicine Rehabilitation. 2007;17(1):43-60.
  47. Wang Ren An. Yifang Ji Jie. Seoul:Sung Bo Sa Publishing Co. 1983:314-6.
  48. Wu Qian and 79 others. Yi Zong Jin Jian. Seoul:Dae Sung Publishing Co. 1983:368.
  49. Han SH, Park SH. Effect of Lycii Fructus Powder on Lipid Metabolism in 1% Cholesterol Fed Rats. Korean Journal of Food Culture. 2008;23(4):521-8.
  50. Park HS, Yang KM, Jung JW. Effect of Water Extract from Hordeum vulgare L. with Medicinal Herb on Plasma Lipid Status and Glucose in Rats Fed High Fat Diet. The Korea Journal of Herbology. 2009;24(1):15-21.
  51. Yoon TS, Sung YY, Jang JY, Yang WK, Ji YU, Kim HK. Anti-obesity Activity of Extract from Saussurea Iappa. Korean Journal of Medicinal Crop Science. 2010;18(3): 151-6.
  52. Kim OG, Joeng JC. Effects of the Mori folium Extract in Streptozotocin-Induced Diabetic Rats. The journal of Korean Oriental Internal Medicine. 2006;27(4):811-21.
  53. Choi JH, Lee HS, Kim YE, Kim BM, Kim IH, Lee CH. Effect of Polygonum multiflorum Thunberg Extract on Lipid Metabolism in Rats Fed High-Cholesterol Diet. The Korean Society of Food Science and Nutrition. 2012;41(7):957-62. https://doi.org/10.3746/jkfn.2012.41.7.957
  54. Park YW, Yang SY, Lee MK, Jin JY, Cho JH. Kim KY. Water Extract of Hovenia dulcis Suppressed Lipid Peroxidation and Improved Renal Function in CCl4 Intoxicated Rats. Korean Journal of Oriental Physiology and Pathology. 2004;18(3):868-73.
  55. Jang JS, Joeng JC. Anti-adipogenic Effecf of Kaempferol, a Component of Polygonati Rhizoma. The Journal of Korean Medical Association. 2010;31(2):158-66.
  56. Shin MK. Clinical Traditional Herbalogy. Seoul:Young Lim Sa Publishing Co. 1997:188, 196, 245, 255, 260, 274, 464, 584, 587, 654, 806.
  57. Guojia Zhongyiyaoguanliju Zhonghua Ben Cao Bianweihui. Zhonghua Ben Cao. Shanghai : Shanghai Kexuejishu chuban. 1999;5:238-41.
  58. Hwang EY, Hong JH, Choi JH, Lee EJ, Lee IS. Study on Anti-obesity and Hypoglycemic Effects of Lycium chinense Mill Extracts. The Korean Society of Food Science and Nutrition. 2009;38(11):1528-34. https://doi.org/10.3746/jkfn.2009.38.11.1528
  59. Cho HJ, Kim DG, Cho KS. Effects of sargassum Extract and Polygoni multiflori radix Extract on the Obese SD Rats induced by 1% Cholesterol Diet. 2001;15(2):1-13.
  60. Eun JS, Hong JS, So JN. Effects of the Extracts from Hoelen alba, Alismatis Rhizoma and Atractylodes Rhizoma on Proliferation and Differentiation of 3T3-L1 Cells. Korean Journal of Pharmacognosy. 1996;24(2):131-9.
  61. Korean Pharmacology Publishing Committee. Korean Pharmacology 2nd edition. Seoul:Shinilbooks Publishing Co. 2007:561.
  62. Kang SA, Jang KH, Hong KH, Choi WA, Jung KH, Lee IY. Effect of Dietary $\beta$-Glucan on Adiposity and Serum Lipids in Obese Rats Induced by High Fat Diet. The Korean Society of Food Science and Nutrition. 2002;31(6):1052-7. https://doi.org/10.3746/jkfn.2002.31.6.1052
  63. MacDougald OA, Hwang C, Fan H, Lane MD. Regulated expression of the obese gene product (leptin) in white adipose tissue and 3T3-L1 adipocytes. Preceedings of the National Academy of Sciences. 1995;92(20):9034-7. https://doi.org/10.1073/pnas.92.20.9034
  64. Gregoire FM, S.C., Sul HS, Understanding adipocyte differentiation. Physiol Rev. 1998;78: 783-809
  65. Cornelius P, MacDougald OA, Lane MD. Regulation of adipocyte development. Annu Rev Nutr. 1994;14: 99-129. https://doi.org/10.1146/annurev.nu.14.070194.000531
  66. Camp HS, Ren D, Leff T. Adipogenesis and fat-cell function in obesity and diabetes. Trends in Molecular Medicine. 2002;8(9):442-7. https://doi.org/10.1016/S1471-4914(02)02396-1
  67. Lee GN, Lee JS. Clinical Pathology Files. Seoul: Medical Culture Publishing Co. 1996:122-6, 150-4.
  68. Chae YH. A Study on Health Examinees' Test Results in Relation to their Obesity Index. Inje University Graduate School of Public Health. 1993.
  69. Kim JC, Kim JJK. Summary of Clinical Examination Methods[revised third edition]. Seoul:Go Mun Publishing Co. 1993:103, 337-45, 420-8, 449-52, 467, 564-7.
  70. Seoul National University College of Medicine. Urology. Seoul:Seoul National University Press. 2008:9-17.
  71. Shin KT. Stepstointernalmedicine. Seoul: Joeng Dam Publishing Co. 2002:49.

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