한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제17권3호
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  • Pages.289-297
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  • 2013
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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Expressional Changes of Water Transport-related Molecules in the Efferent Ductules and Initial Segment of Mouse Treated with Bisphenol A-Containing Drinking Water for Two Generations

  • Han, Su-Yong (College of Liberal Arts and Sciences, Anyang University) ;
  • Lee, Ki-Ho (Department of Biochemistry and Molecular Biology, College of Medicine, Eulji University)
  • 투고 : 2013.09.05
  • 심사 : 2013.09.14
  • 발행 : 2013.09.30
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초록

Bisphenol A (BPA) is an estrogenic endocrine disrupter. However, depending on a way of treatment, the harmful effects of BPA have not been confirmed. Also, trans-generational effects of BPA on male reproduction are still controversial. Because the reabsorption of testicular fluid in the efferent ductules (ED) and initial segment (IS) is important for sperm maturation, the present study was designed to determine trans-generational effect of BPA administrated orally on expression of water transport-related molecules in the mouse ED and IS. Ethanol-dissolved BPA was diluted in water to be 100 ng (low), $10{\mu}g$ (medium), and $1mg/m{\ell}$ water (high). BPA-containing water was provided for two generations. Expression of ion transporters and water channels in the ED and IS were measured by relative real-time PCR analysis. In the ED, BPA treatment caused expressional increases of carbonic anhydrase II, cystic fibrosis transmembrane regulator, $Na^+/K^+$ ATPase ${\alpha}1$ subunit, and aquaporin (AQP) 1. No change of $Na^+/H^+$ exchange (NHE) 3 expression was detected. BPA treatment at medium dose resulted in an increase of AQP9 expression. In the IS, the highest expressional levels of all molecules tested were observed in medium-dose BPA treatment. Generally, high-dose BPA treatment resulted in a decrease or no change of gene expression. Fluctuation of NHE3 gene expression by BPA treatment at different concentrations was detected. These findings suggest that trans-generational exposure to BPA, even at low dose, could affect gene expression of water-transport related molecules. However, such effects of BPA would be differentially occurred in the ED and IS.

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참고문헌

  1. Alonso-Magdalena P, Ropero AB, Soriano S, Garcia-Arevalo M, Ripoll C, Fuentes E, Quesada I, Nadal A (2012) Bisphenol-A acts as a potent estrogen via nonclassical estrogen triggered pathways. Mol Cell Endocrinol 355:201-207. https://doi.org/10.1016/j.mce.2011.12.012
  2. Cagen SZ, Waechter JM Jr, Dimond SS, Breslin WJ, Butala JH, Jekat FW, Joiner RL, Shiotsuka RN, Veenstra GE, Harris LR (1999) Normal reproductive organ development in Winstar rats exposed to bisphenol A in the drinking water. Regul Toxicol Pharmacol 30:130-139. https://doi.org/10.1006/rtph.1999.1340
  3. Clulow J, Jones RC, Hansen LA, Man SY (1998) Fluid and electrolyte reabsorption in the ductuli efferentes testis. J Reprod Fertil Suppl 53:1-14.
  4. Gupta MS (2000) Reproductive malformation of the male offspring following maternal exposure to estrogenic chemicals. Proc Soc Exp Biol. Med 224:61-68.
  5. Howdeshell KL, Furr J, Lambright CR, Wilson VS, Ryan BC, Gray LE Jr (2008) Gestational and lactational exposure to ethinyl estradiol, but not bisphenol A, decreases androgen-dependent reproductive organ weights and epididymal sperm abundance in the male long evans hooded rat. Toxicol Sci 203:371-382.
  6. Kendig EL, Buesing DR, Christie SM, Cookman CJ, Gear RB, Hugo ER, Kasper SN, Kendziorski JA, Ungi KR, Williams K, Belcher SM (2012) Estrogen-like disruptive effects of dietary exposure to bisphenol A or 17a-ethinyl estradiol in CD1 mice. Int J Toxicol 31:537-550. https://doi.org/10.1177/1091581812463254
  7. LaRocca J, Boyajian A, Brown C, Smith SD, Hixon M (2011) Effects of in utero exposure to Bisphenol A or diethylstilbestrol on the adult male reproductive system. Birth Defects Res B Dev Reprod Toxicol 92:526-533. https://doi.org/10.1002/bdrb.20336
  8. Lee KH, Finnigan-Bunick C, Bahr J, Bunick D (2001). Estrogen regulation of ion transporter messenger RNA levels in mouse efferent ductules are mediated differentially through estrogen receptor (ER) alpha and ER beta. Biol Reprod 65:1534-1541. https://doi.org/10.1095/biolreprod65.5.1534
  9. Manikkam M, Guerrero-Bosagna C, Tracey R, Haque MM, Skinner MK (2012) Transgenerational actions of environmental compounds on reproductive disease and identification of epigenetic biomarkers of ancestral exposures. PLoS One 7:e31901. https://doi.org/10.1371/journal.pone.0031901
  10. Miao S, Gao Z, Kou Z, Xu G, Su C, Liu N (2008) Influence of bisphenol A on developing rat estrogen receptors and some cytokines in rats: a two-generational study. J Toxicol Environ Health A 71:1000-1008. https://doi.org/10.1080/15287390801907467
  11. Nanjappa MK, Simon L, Akingbemi, BT (2012) The industrial chemical bisphenol A (BPA) interferes with proliferative activity and development of steroidogenic capacity in rat Leydig cells. Biol Reprod 86:1-12.
  12. Oliveira CA, Carnes K, França LR, Hermo L, Hess RA (2005) Aquaporin-1 and -9 are differentially regulated by oestrogen in the efferent ductile epithelium and initial segment of the epididymis. Biol Cell 97:385-395. https://doi.org/10.1042/BC20040078
  13. Picciarelli-Lima P, Oliveira AG, Reis AM, Kalapothakis E, Mahecha GAB, Hess RA, Oliveira CA (2006) Effect of 3-beta-diol, an androgen metabolite with intrinsic estrogen-like effects, in modulating the aquaporin-9 expression in the rat efferent ductules. Reporod Biol Endocrinol 4:51. https://doi.org/10.1186/1477-7827-4-51
  14. Richter CA, Bimbaum LS, Farabollini F, Newbold RR, Rubin BS, Talsness CE, Vandenbergh JG, Walser-Kuntz DR, Vom Saal FS (2007) In vivo effects of bisphenol A in laboratory rodent studies. Reprod Toxicol 24:199-224. https://doi.org/10.1016/j.reprotox.2007.06.004
  15. Rubin BS (2011) Bisphenol A : an endocrine disrupter with widespread exposure and multiple effects. J Steroid Biochem Mol Biol 127:27-34. https://doi.org/10.1016/j.jsbmb.2011.05.002
  16. Salian S, Doshi T, Vanage G (2009) Perinatal exposure of rats to Bisphenol A affects the fertility of male offspring. Life Sce 85:742-752. https://doi.org/10.1016/j.lfs.2009.10.004
  17. Sheeler CQ, Dudley, MW, Khan SA (2000) Envrionmental estrogens induce transcriptionally active estrogen receptor dimmers in yeast: activity potentiated by the coactivator RIP140. Environ Perspect 108:97-103. https://doi.org/10.1289/ehp.0010897
  18. Spearow JL, Doemeny P, Sera R, Leffler R, Barkley M (1999) Genetic variation in susceptibility to endocrine disruption by estrogen in mice. Science 285:1259-1261. https://doi.org/10.1126/science.285.5431.1259
  19. Takahashi O, Oishi S (2003) Testicular toxicity of dietarily or parenterally administrated bisphenol A in rats and mice. Food Chem Toxicol 41:1035-1044. https://doi.org/10.1016/S0278-6915(03)00031-0
  20. Turner TT (1984) Resorption versus secretion in the rat epididymis. J Reprod Fertil 72:509-514. https://doi.org/10.1530/jrf.0.0720509
  21. Tyl RW, Myers CB, Marr MC, Sloan CS, Castillo NP, Veselica MM, Seely JC, Dimond SS, van Miller JP, Shiotsuk RN, Beyer D, Hentges SG, Waechter JM (2008) Two-generation reproductive toxicity study of dietary bisphenol A in CD-1 (Swiss) mice. Toxicol Sci 104:362-384.
  22. Vanderberg LN, Chauhoud I, Heindel JJ, Padmanabhan V, Paumgartten FJ, Schoenfelder G (2010) Urinary, circulating and tissue biomonitoring studies indicate widespread exposure to bisphenol A. Environ Health Perspect 118:1055-1070. https://doi.org/10.1289/ehp.0901716
  23. vom Saal FS, Cooke PS, Buchanan DL, Palanza P, Thayer KA, Nagel SC, Parmigiani S, Welshons WV (1998) A physiologically based approach to the study of bisphenol A and other estrogenic chemicals on the size of reproductive organs, daily sperm production, and behavior. Toxicol Ind Health 14:2390260.
  24. vom Saal FS, Hughes C (2005) An extensive new literature concerning low-dose effects of bisphenol A shows the need for a new risk assessment. Environ Health Perspect 113:926-933. https://doi.org/10.1289/ehp.7713
  25. Welshones WV, Nagel SC, vom Saal FS (2006) Large effects from small exposures, III. Endocrine mechanisms mediating effects of bisphenol A at levels of human exposure. Endocrinology 147:S56-S69. https://doi.org/10.1210/en.2005-1159
  26. Wong EWP, Cheng CY (2011) Impacts of environmental toxicants on male reproductive dysfunction. Trends Pharmacol Sci 32:290-299. https://doi.org/10.1016/j.tips.2011.01.001