Journal of Wetlands Research (한국습지학회지)

Korean Wetlands Society (한국습지학회)
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Cutting Efficiency Using Phragmites australis Culms According to Content and Timing of Indole-acetic Acid Treatment

옥신 처리 농도 및 시기에 따른 갈대 지상경 삽목 효율

  • Hong, Mun-Gi (Department of Biology Education, Seoul National University) ;
  • Kim, Jae Geun (Department of Biology Education, Seoul National University)
  • Received : 2012.11.21
  • Accepted : 2013.02.23
  • Published : 2013.02.28
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Abstract

In this study, we tried to overcome a limit in cutting timing of reed culm by applying a plant hormone, indole-acetic acid (IAA) as a growth regulator with various contents ($10^{-3}$ M, $10^{-6}$ M, $10^{-9}$ M, $10^{-12}$ M). 19 shoots emerged from 240 segments of hardened reed culm from montane fen and eight out of the 19 shoots emerged by $10^{-6}$ M IAA treatment as the most in $5^{th}$ Sep. 2012. 50 shoots emerged from 60 segments of non-hardened reed culm from a population in Seoul National University transplanted from Mt. Odae by $10^{-6}$ M IAA treatment despite the cutting was performed about two weeks later ($19^{th}$ Sep.). Via third cutting experiment performed about 40 days later ($29^{th}$ Oct.), only two shoots out of 60 segments were observed by the same experimental condition except atmospheric temperature. It seemed likely that it was too low temperature in third experiment ($10^{\circ}C$) than the former experiments (about $20^{\circ}C$) to form adventitious buds from culm segment. We recommend to utilize the thick reed culm in culm cutting as possible because the thicker culm segment we used, the thicker emerged shoot we could observe (i.e., diameters of emerged shoots were about 20% of the planted segment's diameters).

본 연구에서는 갈대 지상경 채취시기와 관련된 제한점을 극복하기 위한 방안으로 가을에 채취한 갈대에 생장조절 식물호르몬인 옥신(IAA)을 다양한 농도($10^{-3}$ M, $10^{-6}$ M, $10^{-9}$ M, $10^{-12}$ M)로 처리하였다. 2012년 9월 5일, 상당 부분 경화가 진행된 산지 습지 갈대에 옥신을 처리한 결과 240개의 절편으로부터 총 19개의 경엽부 출현이 확인되었으며, $10^{-6}$ M에서 옥신의 효과가 가장 크게 나타났다. 산지 습지로부터 이식하여 서울대학교에서 1년간 생육시켜온 갈대의 경우 약 2주 가량 늦게(2012년 9월 19일) 실험을 수행하였음에도 불구하고 $10^{-6}$ M의 옥신 처리 후 60개의 삽목 절편으로부터 총 50개의 경엽부가 출현하였다. 하지만 동일한 방법으로 약 40일 가량 더 경과한 시점인 10월 29일 삽목 실험 결과 60개의 절편으로부터 오직 2개의 경엽부만을 확인할 수 있었다. 앞선 두 실험 시 서울의 기온이 $20^{\circ}C$ 안팎이었던데 반해 세 번째 실험시엔 $10^{\circ}C$ 수준으로 부정아 형성이 이뤄지기엔 다소 낮은 기온이 그 원인으로 보여 진다. 삽목 절편의 직경이 두꺼울수록 출현 경엽부의 직경도 두꺼웠으며(경엽부의 직경은 삽목한 절편 직경의 약 20% 수준이었다.), 이는 더 큰 초고 생장을 가능케 하는 중요한 요소이므로 되도록 두꺼운 갈대 절편을 삽목에 활용하는 것을 제안한다.

Keywords

References

  1. Adcock, PW and Ganf, GG (1994). Growth characteristics of three macrophyte species growing in a natural and constructed wetland system, Water Science and Technology, 29(4), pp. 95-102.
  2. Amoo, SO, Finnie, JF, and Van Staden, J (2009). Effects of temperature, photoperiod and culture vessel size on adventitious shoot production of in vitro propagated Huernia hystrix, Plant Cell, Tissue and Organ Culture, 99(2), pp. 233-238. https://doi.org/10.1007/s11240-009-9592-0
  3. Asaeda, T and Karunaratne, S (2000). Dynamics modeling of the growth of Phragmites australis: Model description, Aquatic Botany, 67(4), pp. 301-318. https://doi.org/10.1016/S0304-3770(00)00095-4
  4. Chambers, PA (1987). Light and nutrients in the control of aquatic plant community structure. II. In situ Observation, J. of Ecology, 75(3), pp. 621-628. https://doi.org/10.2307/2260194
  5. Choung, YS, Oh, HK, Roh, CH, and Hwang, KS (1999). Shoot cutting effects on the productivity and nutrient removal of some wetland plants, Korean J. of Environmental Biology, 17(4), pp. 459-465. [Korean Literature]
  6. Haslam, SM (1970). The performance of Phragmites communis Trin. in relation to water-supply, Annals of Botany, 34(4), pp. 867-877. https://doi.org/10.1093/oxfordjournals.aob.a084418
  7. Hogg, EH and Wein, RW (1987). Growth dynamics of floating Typha mats: Seasonal translocation and internal deposition of organic material, Oikos, 50(2), pp. 197-205. https://doi.org/10.2307/3566001
  8. Hong, MG and Kim, JG (2012). Growth characteristics of cutting culms sectioned at different positions from three reed population, J. of Korean Environmental Restoration and Technology, 15(1), pp. 53-62. [Korean Literature]
  9. Hong, M-G, and Kim, JG (2011). Comparative analysis of cutting efficiency using culms of reed with genetic, environmental and methodological differences, J. of Wetlands Research, 13(3), pp. 603-611. [Korean Literature]
  10. Hwang, IT, Choi, JS, Lee, HJ, Hong, KS, and Cho, KY (1996). Establishment of herbicide screening methods for reed (Phragmites communis Trin.) control - 1. Propagation of reed -, Korean J. of Weed Science, 16(1), pp. 21-27. [Korean Literature]
  11. Jeong, DY and Shim, SR (1999). A study on the development of planting methods for Phragmites spp., J. of the Korean Institute of Landscape Architecture, 27(2), pp. 51-57. [Korean Literature]
  12. Jeong, GJ, Kim, MG, and An, WY (2003). A fundamental study on the effect to build up a vegetation strip at stream confluence by using reed mat, J. of Korean Environmental Restoration and Technology, 6(4), pp. 62-73. [Korean Literature]
  13. Ju, YG (1998). A study on the planting methods of reeds in the Tideland, J. of Korean Institute of Traditional Landscape Architecture, 16(3), pp. 35-40. [Korean Literature]
  14. Kang, YKi, Ko, MR, Kang, SY, and Riu, KZ (2005). Several factors affecting to rooting of stem cuttings in Rubus Buergeri Miquel, Korean J. of Medicinal Crop Science, 13(3), pp. 77-80. [Korean Literature]
  15. Kato, Y and Ozawa, N (1978). Adventitious bud formation on leaf and stem segments of Heloniopsis orientalis grown at various temperatures, Plant and Cell Physiology, 20(3), pp. 491-497.
  16. Kim, C-S and Kim, Z-S (2007). Effects of auxin and fog treatments on the green-wood cutting of the mature trees in Prunus yedoensis, J. of Korean Forest Society, 96(6), pp. 676-683. [Korean Literature]
  17. Kim, IJ, Kim, MJ, Nam, SY, Lee, CH, and Kim, HS (2004). Effects of bedsoil and growth regulator on cutting propagation of Cudrania Tricuspidata bureau, Korean J. of Medicinal Crop Science, 12(4), pp. 285-288. [Korean Literature]
  18. Klimes, L (2000). Phragmites australis at an extreme altitude: Rhizome architecture and its modelling, Folia Geobotanica, 35(4), pp. 403-417. https://doi.org/10.1007/BF02803552
  19. League, MT, Colbert, EP, Seliskar, DM, and Gallagher, JL (2006). Rhizome growth dynamics of native and exotic haplotypes of Phragmites australis (common reed), Estuaries and Coasts, 29(2), pp. 269- 276. https://doi.org/10.1007/BF02781995
  20. Lee, GM and Kim, JG (2012). Effects of rhizome length and node numbers on the proliferation of Menyanthes Trifoliata cuttings, J. of Wetlands Research, 14(2), pp. 193-198. [Korean Literature]
  21. Lee, HHM, Kwon, OB (2002). Development of floatingislands with a sod mat by shooting and rooting from shoot nodes of common reed, J. of Korean Environmental Restoration and Technology, 5(1), pp. 59-65. [Korean Literature]
  22. Lee, HHM, Kwon, OB, Seog, JH, and Jo, GH (2001). Selection of suitable plants for artificial floating islands - Comparisons of vegetation structure and growth of four emergent macrophytes-, J. of Korean Environmental Restoration and Technology, 4(1), pp. 87-96. [Korean Literature]
  23. Lee, JE, Seo, SG, Kim, BK, Woo, SM, Koo, BC, Park, TH, Lim, YP, and Kim, SH (2012). Induction of somatic embryogenesis and plant regeneration in the reed grass (Phragmites communis Trin.), African J. of Biotechnology, 11(8), pp. 1904-1911.
  24. Oh, KH and Ihm, BS (1983). Seasonal changes in the productivity and soil nutrients of Phragmites communis Community in the salt marsh of the sumjin - River Estuary, Korean J. of Ecology, 6(2), pp. 90-97. [Korean Literature]
  25. Rejmankova, E (2011). The role of macrophytes in wetland ecosystems, J. of Ecology and Field Biology, 34(4), pp. 333-345. https://doi.org/10.5141/JEFB.2011.044
  26. Ro, HM, Choi, WJ, Lee, EJ, Yun, SI, and Choi, YD (2002). Uptake patterns of N and P by reeds (Phragmites australis) of newly constructed Shihwa tidal freshwater marshes, Korean J. of Ecology, 25(5), pp. 359-364. [Korean Literature] https://doi.org/10.5141/JEFB.2002.25.5.359
  27. Spence, DHN (1982). The zonation of plants in freshwater lakes, Advances in Ecological Research, 12, pp. 37-125. https://doi.org/10.1016/S0065-2504(08)60077-X
  28. Straub, PF, Decker, DM, and Gallagher, JL (1988). Tissue culture and long-term regeneration of Phragmites australis (Cav.) Trin. ex Steud, Plant Cell, Tissue and Organ Culture, 15(1), pp. 73-78. https://doi.org/10.1007/BF00039891
  29. Yang, Y-G, Guo, Y-M, Guo, Y, Guo, Z-C, and Lin, J-X (2003). Regeneration and large-scale propagation of Phragmites communis through somatic embryogenesis, Plant Cell, Tissue and Organ Culture, 75(3), pp. 287-290. https://doi.org/10.1023/A:1025871015405

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