Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Developmental Abnormalities in Zebrafish Angiogenesis with Chronic Exposure to Crude Oil and Dispersant

  • Received : 2012.09.25
  • Accepted : 2013.01.17
  • Published : 2013.03.31
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Abstract

Oil spills have occurred throughout the years of industrialization and represent a global challenge as they affect vast areas of the ocean. The toxicity of crude oil to aquatic organisms has been extensively investigated, but the potential impacts of crude oil on vertebrate development remain largely unknown. Here, we investigated the effects of dispersants used in treating a recent oil spill, as well as that of crude oil, on vertebrates by using the zebrafish (Danio rerio) model species, which has been widely used in empirical studies of both early embryonic development and adult physiology. Chronic exposure to crude oil resulted in marked developmental abnormalities, including pericardial edema, abnormal trunk vessel development, retardation of axonal branching, and abnormal jaw development. Embryonic development was affected more severely by exposure to the oil-dispersant combination than to the oil alone. Thus, the zebrafish in vivo model system suggests that dispersant treatment can have detrimental developmental effects on vertebrates and its potential impact on marine life, as well as humans, should be carefully considered in clean-up efforts at the site of an oil spill.

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References

  1. Briggs KT, SH Yoshida and ME Gershwin. 1996. The influence of petrochemicals and stress on the immune system of seabirds. Regul. Toxicol. Pharmacol. 23:145-155. https://doi.org/10.1006/rtph.1996.0036
  2. Broje V and AA Keller. 2006. Improved mechanical oil spill recovery using an optimized geometry for the skimmer surface. Environ. Sci. Technol. 40:7914-7918. https://doi.org/10.1021/es061842m
  3. Chapman H, K Purnell, RJ Law and MF Kirby. 2007. The use of chemical dispersants to combat oil spills at sea: A review of practice and research needs in Europe. Mar. Pollut. Bull. 54:827-838. https://doi.org/10.1016/j.marpolbul.2007.03.012
  4. Fleeger JW, KR Carman and RM Nisbet. 2003. Indirect effects of contaminants in aquatic ecosystems. Sci. Total Environ. 317:207-233. https://doi.org/10.1016/S0048-9697(03)00141-4
  5. Jin SW, D Beis, T Mitchell, JN Chen and DY Stainier. 2005. Cellular and molecular analyses of vascular tube and lumen formation in zebrafish. Development 132:5199-5209. https://doi.org/10.1242/dev.02087
  6. Kim JH. 2007. http://news.naver.com/main/read.nhn?mode= LSD&mid=sec&sid1=102&oid=001&aid=0001872055 YonhapNews.
  7. Mehlman MA and MS Legator. 1991. Dangerous and cancercausing properties of products and chemicals in the oil refining and petrochemical industry-Part II: Carcinogenicity, mutagenicity, and developmental toxicity of 1,3-butadiene. Toxicol. Ind. Health. 7:207-220. https://doi.org/10.1177/074823379100700306
  8. Michel J, G Shigenaka and R Hoff. 2010. Chapter 5 - Oil Spill Response and Cleanup Techniques. National Oceanic and Atmospheric Administration (NOAA). Retrieved May 21.
  9. Pauka LM, M Maceno, SC Rossi and HC Silva de Assis. 2011. Embryotoxicity and biotransformation responses in zebrafish exposed to water-soluble fraction of crude oil. Bull. Environ. Contam. Toxicol. 86:389-393. https://doi.org/10.1007/s00128-011-0235-x
  10. Ramseur JL. 2012. Oil Spills in U.S. Coastal Waters: Background and Governance. Congressional Research Service. 7-5700.
  11. Robert S. 1998. Oil spill response performance review of skimmers. Vol. 34 /ASTM manual series, ASTM International. Schrope M. 2011. Oil spill: deep wounds. Nature 472:152-154. https://doi.org/10.1038/472152a
  12. Singer MM, D Aurand, GE Bragin, JR Clark, GM Coelho, ML Sowby and RS Tjeerdema. 2000. Standardization of the Preparation and Quantitation of Water-accommodated Fractions of Petroleum for Toxicity Testing. Mar. Pollut. Bull. 40: 1007-1016. https://doi.org/10.1016/S0025-326X(00)00045-X
  13. Son SW, JH Kim, SH Kim, H Kim, AY Chung, JB Choo, CH Oh and HC Park. 2009. Intravital imaging in zebrafish using quantum dots. Skin Res. Technol. 15:157-160. https://doi.org/10.1111/j.1600-0846.2008.00341.x
  14. Westfield M. 1995. The Zebrafish Book. Univ. of Oregon, Eugene, OR.
  15. Wolf CP. 2008. The Taean International Environment Forum on "Lessons Learnt from the Taean Oil Spill & Marine Conservation', Anmyundo, Taean, Chungnam Province, Korea.
  16. Yeo SY, M Kim, HS Kim, TL Huh and AB Chitnis. 2007. Fluorescent protein expression driven by her4 regulatory elements reveals the spatiotemporal pattern of Notch signaling in the nervous system of zebrafish embryos. Dev. Biol. 301: 555-567. https://doi.org/10.1016/j.ydbio.2006.10.020