Economic and Environmental Geology (자원환경지질)

The Korean Society of Economic and Environmental Geology (대한자원환경지질학회)
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Characterization of Water Quality and Bacteria of Leachate from Animal Carcass Disposal on the Disposal Lapse Time

매몰 시간의 경과에 따른 구제역 가축 매몰지 침출수 특성에 관한 연구

  • Choi, Nag-Choul (Department of Rural Systems Engineering/Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Choi, Eun-Ju (Department of Energy and Resource Engineering, Chosun University) ;
  • Kim, Bong-Ju (Department of Energy and Resource Engineering, Chosun University) ;
  • Park, Jeong-Ann (Department of Rural Systems Engineering/Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Song-Bae (Department of Rural Systems Engineering/Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Park, Cheon-Young (Department of Energy and Resource Engineering, Chosun University)
  • 최낙철 (서울대학교 지역시스템공학과) ;
  • 최은주 (조선대학교 에너지자원공학과) ;
  • 김봉주 (조선대학교 에너지자원공학과) ;
  • 박정안 (서울대학교 지역시스템공학과) ;
  • 김성배 (서울대학교 지역시스템공학과) ;
  • 박천영 (조선대학교 에너지자원공학과)
  • Received : 2013.06.02
  • Accepted : 2013.08.11
  • Published : 2013.08.28
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Abstract

In this study, the physicochemical properties of leachate and the bacteria existence in leachate using molecular biology methods for 4 animal carcass disposals on the disposal lapse time was analyzed. The result of leachate physicochemical analysis in the middle stage (been buried 20 months) showed higher EC, DO, $HCO_3{^-}$, TOC, T-N and $SO_4{^{2-}}$ concentration compared to the first stage data (been buried 5 months). For identification of leachate using 16S rRNA method, Lysinibacillus sphaericus, Bacillus pumilus, Pseudoclavibacter helvolus, Pseudochrobactrum saccharolyticum and Corynebacterium callunae in the first stage, Bacillus cereus, Lysinibacillus sphaericus, Bacillus circulans and Corynebacterium glutamicum in the middle stage was observed, while there were detections of pathogenicity bacteria such as B. cereus and L. sphaericus. This study improves our knowledge of the fate and transport in geologic media, treatment, risk analysis on the leachate from animal carcass disposal sites.

본 연구에서는 매몰경과시간에 따라 4지점의 가축매몰지 침출수에 대하여 물리화학적 특성과 분자미생물학적 방법을 이용하여 침출수내 미생물을 분석하였다. 침출수에 대한 물리화학적 분석 결과, 매몰중기 (매몰경과 20개월)에서 매몰초기 (매몰경과 5개월) 자료에 비하여 EC, DO, $HCO_3{^-}$, TOC, T-N 과 $SO_4{^{2-}}$ 의 함량이 높게 나타났다. 16S rRNA방법을 이용한 침출수의 미생물을 동정한 결과, 매몰초기에 Lysinibacillus sphaericus, Bacillus pumilus, Pseudoclavibacter helvolus, Pseudochrobactrum saccharolyticum 과 Corynebacterium callunae 이 동정되었으며, 매몰중기에는 Bacillus cereus, Lysinibacillus sphaericus, Bacillus circulans 과 Corynebacterium glutamicum이 검출되었고, Bacillus cereus, Lysinibacillus sphaericus과 같은 병원성 미생물이 발견되었다. 본 연구는 가축매몰지로부터 형성되는 침출수에 대한 지질 매체 내에서의 거동특성, 처리 및 위해성 평가에 대한 기초자료로 활용 가능할 것이다.

Keywords

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