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남해안 패류양식장에서 분리된 대장균(Escherichia coli)의 항균제 내성

Antimicrobial Resistance in Escherichia coli Isolated from the Shellfish Farms in the Southern Coast of Korea

  • 박큰바위 (국립수산과학원 식품안전과) ;
  • 박준용 (국립수산과학원 식품안전과) ;
  • 조미라 (국립수산과학원 식품안전과) ;
  • 유홍식 (국립수산과학원 식품안전과) ;
  • 이희정 (국립수산과학원 식품안전과) ;
  • 김지회 (국립수산과학원 식품안전과) ;
  • 오은경 (국립수산과학원 서해수산연구소) ;
  • 신순범 (국립수산과학원 남서해수산연구소) ;
  • 김연계 (국립수산과학원 남서해수산연구소) ;
  • 이태식 (국립수산과학원 남서해수산연구소)
  • Park, Kunbawui (Food Safety Research Division, National Fisheries Research and Department Institude) ;
  • Park, Jun Yong (Food Safety Research Division, National Fisheries Research and Department Institude) ;
  • Jo, Mi Ra (Food Safety Research Division, National Fisheries Research and Department Institude) ;
  • Yu, Hong Sik (Food Safety Research Division, National Fisheries Research and Department Institude) ;
  • Lee, Hee Jung (Food Safety Research Division, National Fisheries Research and Department Institude) ;
  • Kim, Ji Hoe (Food Safety Research Division, National Fisheries Research and Department Institude) ;
  • Oh, Eun Gyoung (West Sea Fisheries Research Institute, National Fisheries Research and Department Institude) ;
  • Shin, Soon Bum (Southwest Sea Fisheries Research Institute, National Fisheries Research and Department Institude) ;
  • Kim, Yeon Kye (Southwest Sea Fisheries Research Institute, National Fisheries Research and Department Institude) ;
  • Lee, Tae Seek (Southwest Sea Fisheries Research Institute, National Fisheries Research and Department Institude)
  • 투고 : 2013.08.12
  • 심사 : 2013.09.09
  • 발행 : 2013.10.31

초록

This study evaluated the impact of antimicrobial resistant bacteria produced by inland pollution sources on coastal areas, and investigates antimicrobial resistance profiles of bacteria isolated from marine filter feeders. A total of 107 shellfish (short-necked clams Ruditapes philippinarum, ark shells Scapharca broughtonii, and oysters Crassostrea gigas) were collected from the southern coast of Korea, from which 204 Escherichia coli strains were isolated. The antimicrobial susceptibility patterns of isolated strains were analyzed for 15 antimicrobial agents used in Korea for clinical or veterinary therapy. Antimicrobial resistance was present in 44.6% of E. coli isolates against at least one antimicrobial agent. The rate of antimicrobial resistance in the Narodo area was higher than isolates from the Gangjinman area and Kamak Bay. E. coli isolates had a higher rate of resistance against: tetracycline (29.9%), streptomycin (25.5%), and trimethoprim (14.2%). Of 204 isolates, 29 (14.2%) were resistant to multiple antimicrobial agents.

키워드

참고문헌

  1. Acar JF and Goldstein FW. 1991. Disk susceptibility test. In: Antibiotics in Laboratory Medicine, Lorian, V. Ed., Williams & Wilkins, Baltimore, U.S.A., 17-52.
  2. A.P.H.A. 1970. Recommended procedures for the examination of seawater and shellfish. 4th Ed. American Public Health Association, Washington D.C., U.S.A., 1-47.
  3. Chae HS, Kim JH, Kim GH, Choi TS, Shin BW, Lee DJ and Lee JH. 2005. The isolation and antimicrobial susceptibility of Escherichia coli O157:H7 on bovine feces and carcass. Korean J Vet Serv 28, 71-79.
  4. Cho JK, Ha JS and Kim KS. 2006. Antimicrobial drug resistance of Escherichia coli isolated from cattle, swine and chicken. Kor J Vet Publ Hlth 30, 9-18.
  5. Choi SH, Lee YJ, Kim BH, Kim KS, Park CK, Bae DH, Cho JK, Kim JW, Kim BH and Kang MS. 2006. Antibiotic resistance and genotypic characterization of Escherichia coli isolated from pig farm environment. J Bacteriol Virol 36, 159-165. https://doi.org/10.4167/jbv.2006.36.3.159
  6. CLSI. 2004. Performance standards for antimicrobial susceptibility testing. CLSI document M100-S14. Clinical and Laboratory Standards Institute. Wayne, PA. U.S.A., 29-76.
  7. Feldhusen F. 2000. The role of seafood in bacterial foodborne disease. Microbes Infect 2, 1651-1660. https://doi.org/10.1016/S1286-4579(00)01321-6
  8. Gaskins HR, Collier CT and Anderson DB. 2002. Antibiotics as growth promotants: mode of action. Anim Biothechnol 13, 29-42. http://dx.doi.org/10.1081/ABIO-120005768.
  9. Grimes DJ. 1991. Ecology of estuarine bacteria capable of causing human disease: A review. Estuaries 14, 345-360. https://doi.org/10.2307/1352260
  10. Herwig RP, Gray JP and Weston DP. 1997. Antibacterial resistant bacteria in surficial sediments near salmon net-cage farms in Puget Sound. Washington Aquaculture 149, 263-283.
  11. Hill DD, Owens WE and Tchounwou PB. 2006. The impact of rainfall on fecal coliform bacteria in Bayou Dorcheat (North Louisiana). Int J Environ Public Health 3, 114-117. http://dx.doi.org/10.3390/ijerph2006030013.
  12. Kim MS, Kwon HM and Sung HW. 2009. Antibiotic resistance pattern of avian pathogenic Escherichia coli isolated from chickens. Korean J Vet Res 49, 195-200.
  13. Kummerer K. 2009. Antibiotics in the aquatic environment - A review - Park I. Chemosphere 75, 417-434. https://doi.org/10.1016/j.chemosphere.2008.11.086
  14. Kwon JY, Park K, Song KC, Lee HJ, Park JH, Kim JD and Son KT. 2007. Evaluation of the bacteriological quality of a shellfish-growing area in kamak bay, Korea. Kor J Fish Aquat Sci 11, 7-14. https://doi.org/10.5657/fas.2008.11.1.007
  15. Lee TS, Oh EG, Yu HD, Ha KS, Yu HS, Byun HS and Kim JH. 2010. Impact of rainfall events on the bacteriological water quality of the shellfish growing area in Korea. Kor J Fish Aquat Sci 43, 406-414. https://doi.org/10.5657/kfas.2010.43.5.406
  16. Lee YJ, Kim AR, Jung SC, Song SW and Kim JH. 2005. Antibiotic resistance pattern of E. coli and Salmonella spp. Isolated from chicken feces. Korean J Vet Serv 45, 75-83.
  17. Levin BR, Lipsitch M, Perrot V, Schrag S, Antia R, Simonsen L, Walker NM and Stewart FM. 1997. The population genetics of antibiotic resistance. Clin Infect Dis 24, 9-16. https://doi.org/10.1093/clinids/24.Supplement_1.S9
  18. Oh EG, Son KT, Yu HS, Kim JH, Lee TS and Lee HJ. 2008. Antimicrobial susceptibility patten of Enterococcus faecalis and E. faecium from fish farms in the southern coast of Korea. J Kor Fish Soc 41, 435-439. https://doi.org/10.5657/kfas.2008.41.6.435
  19. Park K, Jo MR, Lee HJ, Kwon JY, Son KT and Lee TS. 2011. Evaluation of the effect of the dischatged water from Bong stream after events on the bacteriological water quality in Gangjinman, Korea. Kor J Fish Aquat Sci 44, 622-629. https://doi.org/10.5657/KFAS.2011.0622
  20. Park K, Jo MR, Kim YK, Lee HJ, Kwon JY, Son KT and Lee TS. 2012. Evaluation of the effects of the inland pollution sources after rainfall events on the bacteriological water quality in Narodo area, Korea. Kor J Fish Aquat Sci 45, 414-422. Http://dx.doi.org/10.5657/KFAS.2012.0414.
  21. Son KT, Oh EG, Lee TS, Lee HJ, Kim PH and Kim JH. 2005. Antimicrobial susceptibility of Vibrio parahaemolyticus and Vibrio alginolyticus from fish farms on the southern coast of Korea. J Kor Fish Soc 38, 365-371. https://doi.org/10.5657/kfas.2005.38.6.365
  22. Son KT, Oh EG, Park K, Kwon JY, Lee HJ, Lee TS and Kim JH. 2009. Antimicrobial susceptibility of Escherichia coli isolated from fish farms on the southern coast of Korea. Kor J Fish Aquat Sci 42, 322-328. https://doi.org/10.5657/kfas.2009.42.4.322
  23. Tendencia EA and Pena LD. 2001. Antibiotic resistance of bacteria from shrimp ponds. Aquaculture 195, 193-204. https://doi.org/10.1016/S0044-8486(00)00570-6
  24. Tollefson L, Altekruse SF and Potter. 1997. Therapeutic antibiotics in animal feeds and antibiotic resistance. Rev Sci Tech 16, 709-715. https://doi.org/10.20506/rst.16.2.1054

피인용 문헌

  1. Antimicrobial Resistance in Escherichia coli Isolated from Shellfish Farms on the West Coast of Korea vol.49, pp.1, 2016, https://doi.org/10.5657/KFAS.2016.0013