Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
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The Effect of Silk Fibroin Particles Coated with Hydroxyapatites on Bone Regeneration in the Rat Calvarial Defect Model

백서 두개골 결손모델에서 하이드록시아파타이트 입자로 입혀진 실크단백이 골재생에 미치는 영향

  • Seok, Hyun (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Park, Young-Tae (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Kim, Seong-Gon (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Jin, Hyung-Joon (Department of Polymer Science and Engineering, Inha University)
  • 석현 (강릉원주대학교 치과대학 구강악안면외과학교실) ;
  • 박용태 (강릉원주대학교 치과대학 구강악안면외과학교실) ;
  • 김성곤 (강릉원주대학교 치과대학 구강악안면외과학교실) ;
  • 진형준 (인하대학교 고분자신소재공학과)
  • Received : 2012.12.19
  • Accepted : 2013.01.24
  • Published : 2013.01.31
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Abstract

Purpose: This study evaluated the capability of bone formation of silk fibroin particles coated with hydroxyapatites (HA/SF), as bone graft material when put into the calvarial defect of rats. Methods: Twenty Sprague Dawley rats were used for this study and round shaped defects were formed in the center of parietal bones (diameter: 8.0 mm). The defect was filled with (1) HA/SF (experimental group), or (2) left as a vacant space (control group). The animals were sacrificed at 4 or 8 weeks, postoperatively. The specimens were decalcified and stained with Masson's trichrome for histomorphometric analysis. Results: The average of new bone formation was $33.18{\pm}3.10%$ in the experimental group and $20.49{\pm}5.79%$ in the control group at 4 weeks postoperatively. That was $42.52{\pm}7.74%$ in the experimental group and $25.50{\pm}7.31%$ in the control group at 8 weeks postoperatively. The difference between the groups was significantly higher at both 4 weeks and 8 weeks postoperatively (P<0.05). Conclusion: The rat calvarial defect was successfully repaired by HA/SF graft. The HA/SF graft showed more new bone formation compared with the unfilled control.

Keywords

References

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