Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Antimelanogenic Effect and Whitening of Anthocyanin Rich Fraction from Seeds of Liriope platyphylla

맥문동 종실 안토시아닌 분획물의 멜라닌 생성 억제 및 미백 효과

  • Choung, Myoung Gun (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Hwang, Young Sun (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Kim, Gi Ppeum (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Ahn, Kyung Geun (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Shim, Hoon Seob (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Hong, Seung Beom (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Choi, Jae Hoo (Department of Applied Plant Sciences, Kangwon National University) ;
  • Yu, Chang Yeon (Department of Applied Plant Sciences, Kangwon National University) ;
  • Chung, Ill Min (Department of Applied Bioscience, Konkuk University) ;
  • Kim, Seung Hyun (Department of Applied Bioscience, Konkuk University) ;
  • Lim, Jung Dae (Department of Herbal Medicine Resource, Kangwon National University)
  • 정명근 (강원대학교 생약자원개발학과) ;
  • 황영선 (강원대학교 생약자원개발학과) ;
  • 김기쁨 (강원대학교 생약자원개발학과) ;
  • 안경근 (강원대학교 생약자원개발학과) ;
  • 심훈섭 (강원대학교 생약자원개발학과) ;
  • 홍승범 (강원대학교 생약자원개발학과) ;
  • 최재후 (강원대학교 식물자원응용공학과) ;
  • 유창연 (강원대학교 식물자원응용공학과) ;
  • 정일민 (건국대학교 응용생물과학과) ;
  • 김승현 (건국대학교 응용생물과학과) ;
  • 임정대 (강원대학교 생약자원개발학과)
  • Received : 2013.08.27
  • Accepted : 2013.09.26
  • Published : 2013.10.30
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Abstract

This study was performed to determine the antimelanogenic effect and tyrosinase inhibitory activities of anthocyanin rich fraction (AN-SLP) from Liriope platyphylla Wang et Tang seeds. Anthocyanins isolated from L. platyphylla seeds revealed the presence of four major anthocyanin components, which were tentatively identified as delphinidin-3-Oglucoside, delphinidin-3-O-rutinoside, petunidin-3-O-rutinoside, and malvidin-3-O-rutinoside using semipreparative HPLC, $^1H$-NMR, $^{13}C$ NMR, FAB-MS and LC/ES-MS. The inhibitory effect of AN-SLP on tyrosinase activity was studied using in vitro (against mushroom tyrosinase) and ex vivo (against B16 melanoma cell tyrosinase) models. Cellular tyrosinase activity was decreased by AN-SLP treatment in B 16 melanoma cells through dose dependent manner, but AN-SLP did not inhibit mushroom tyrosinase and L-DOPA oxidation directly. AN-SLP showed melanin inhibition by 53.2% at 50 ${\mu}g/m{\ell}$ which was 0.7 times more efficient than the antimelanogenic effect of commercial arbutin and kojic acid (36.5%) also did not show cell toxicity. Additionally, AN-SLP inhibited the activity of ${\alpha}$-glucosidase and the glycosylation of tyrosinase in melanoma cell. The resulting unsaturated glycosylation of tyrosinase makes it unstable and disturb correct transportation. From theses results, we conclude that AN-SLP could be used as anti-melanogenic agent for skin whitening.

Keywords

References

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