Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Characteristics of Leaf Morphology and Genetic Variation of the Rare Woody Plant, Berchemia racemosa var. magna

희귀수종 먹넌출 엽의 형태적 특성과 유전변이

  • Song, Jeong-Ho (Division of Special-purpose Trees, Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Lim, Hyo-In (Division of Forest Genetic Resources, Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Jang, Kyung-Hwan (Division of Forest Genetic Resources, Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Han, Jingyu (Division of Forest Genetic Resources, Department of Forest Genetic Resources, Korea Forest Research Institute)
  • 송정호 (국립산림과학원 산림유전자원부 특용자원연구과) ;
  • 임효인 (국립산림과학원 산림유전자원부 산림유전자원과) ;
  • 장경환 (국립산림과학원 산림유전자원부 산림유전자원과) ;
  • 한진규 (국립산림과학원 산림유전자원부 산림유전자원과)
  • Received : 2013.04.11
  • Accepted : 2013.10.16
  • Published : 2013.10.31
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Abstract

This study was conducted to investigate the morphological characteristics of leaf and the genetic diversity of Berchemia racemosa var. magna which is only found in Anmyeon Island of South Korea. ANOVA test showed that there were significant differences among individuals within population in all 10 leaf characteristics. Average characteristics of 39 individuals were 11.8 cm in leaf length, 7.1 cm in leaf width, 1.67 in leaf index, 5.4 cm in upper 1/3 width, 6.2 cm in lower 1/3 width, 3.6 cm in petiole length, 0.19 mm in leaf thickness, 11.5 ea. in number of veins (left), 11.4 ea. in number of veins (right) and 61.7 $cm^2$ in leaf area, respectively. Except for leaf thickness (18.8%), petiole length (21.7%) and leaf area (22.0%), the coefficients of variation of most leaf characteristics were relatively low (<15.0%). A total of 50 bands was generated from 8 selected I-SSR primers. The estimates of genetic variation were 1.719 in effective number of alleles ($A_e$), 26.0% in proportion of polymorphic bands (P), 0.410 in expected heterozygosity ($H_e$) and 0.598 in Shannon's diversity index (S.I.), respectively. In spite of the small number and the limited distribution, the B. racemosa var. magna population in Anmyeon Island showed high genetic diversity.

우리나라에서 안면도에만 분포하는 먹넌출 집단의 생명자원보존을 위하여 엽의 형태적 특성과 I-SSR 표지자를 이용한 유전변이를 조사하였다. 10가지 엽특성에 대한 ANOVA 분석결과 모든 특성에서 개체 간에 통계적인 유의성이 인정되었다. 조사된 39개체의 평균특성은 엽신장 11.8 cm, 최대엽폭 7.1 cm, 엽지수 1.67, 상1/3폭 5.4 cm, 하1/3폭 6.2 cm, 엽병길이 3.6 cm, 엽두께 0.19 mm, 엽맥수(좌) 11.5개, 엽맥수(우) 11.4개, 엽면적 61.7 $cm^2$로 나타났다. 변이계수 값은 엽두께, 엽병길이, 엽면적이 각각 18.8%, 21.7%, 22.0%로 높게 나타났으며, 나머지 특성들에서는 15% 이내의 비교적 낮은 변이를 나타냈다. 선발된 8개 I-SSR Primer에서 총 50개의 증폭산물을 얻었으며, 유효대립 유전자의 수 1.719개, 다형적 유전자좌의 비율 26.0%, 이형접합도의 기대치 0.410 및 Shannon의 다양성지수 0.598로 각각 나타났다. 안면도 먹넌출 집단은 제한된 지역에 분포하며 개체수가 적음에도 불구하고 높은 유전다양성을 유지하는 것으로 나타났다.

Keywords

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