Asian Pacific Journal of Cancer Prevention

  • 제14권8호
  • /
  • Pages.4651-4654
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  • 2013
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Chloroquine and Valproic Acid Combined Treatment in Vitro has Enhanced Cytotoxicity in an Osteosarcoma Cell Line

  • Wang, Chuan-Kun (Department of Orthopaedics, The Fourth Hospital of Harbin) ;
  • Yu, Xi-Dong (Department of Orthopaedics, The Fourth Hospital of Harbin) ;
  • Li, Qiang (Department of Orthopaedics, The Fourth Hospital of Harbin) ;
  • Xie, Gang (Department of Orthopaedics, The Fourth Hospital of Harbin) ;
  • Teng, Yue (Department of Orthopaedics, The Fourth Hospital of Harbin)
  • 발행 : 2013.08.30
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초록

Choroquine (CQ) and valproic acid (VPA) have been extensively studied for biological effects. Here, we focused on efficacy of combined CQ and VPA on osteosarcoma cell lines. Viability of osteosarcoma cell lines (U20S and HOS) was analyzed by MTT assay. Apoptotic assays and colony formation assays were also applied. ROS generation and Western Blotting were performed to determine the mechanism of CQ and VPA combination in the process of apoptosis. The viability of different osteosarcoma cell lines significantly decreased after CQ and VPA combination treatment compared with either drug used alone, and apoptosis was increased significantly. ROS generation was triggered leading to expression of apoptosis related genes being increased and of antiapoptotic related genes being decreased. From our data shown here, CQ and VPA combination treatment in vitro enhanced cytotoxicy to osteosarcoma cells.

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참고문헌

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피인용 문헌

  1. Gemcitabine for the Treatment of Patients with Osteosarcoma vol.15, pp.17, 2014, https://doi.org/10.7314/APJCP.2014.15.17.7159
  2. Biological Screening of Novel Derivatives of Valproic Acid for Anticancer and Antiangiogenic Properties vol.15, pp.18, 2014, https://doi.org/10.7314/APJCP.2014.15.18.7785