Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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MicroRNA-497 Suppresses Proliferation and Induces Apoptosis in Prostate Cancer Cells

  • Wang, Li (Department of Urology, Affiliated Hospital of Hebei, University of Engineering) ;
  • Li, Bo (Department of Urology, Affiliated Hospital of Hebei, University of Engineering) ;
  • Li, Lei (Department of General Surgery, Affiliated Hospital of Hebei, University of Engineering) ;
  • Wang, Te (School of Government of Beijing Normal University)
  • Published : 2013.06.30
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Abstract

MicroRNAs (miRNAs) are a class of endogenously expressed small, non-coding, single-stranded RNAs that negatively regulate gene expression, mainly by binding to 3'- untranslated regions (3'UTR) of their target messenger RNAs (mRNAs), which cause blocks of translation and/or mRNA cleavage. Recently, miRNAprofiling studies demonstrated the microRNA-497 (miR-497) level to be down-regulated in all prostate carcinomas compared with BPH samples. The purpose of this study was to investigate the potential role of miR-497 in human prostate cancer. Proliferation, cell cycle and apoptosis assays were conducted to explore the potential function of miR-497 in human prostate cancer cells. Results showed that miR-497 suppressed cellular growth and initiated G0/G1 phase arrest of LNCaP and PC-3 cells. We also observed that miR-497 increased the percentage of apoptotic cells by increasing caspase-3/7 activity. Taken together, our results demonstrated that miR-497 can inhibit growth and induce apoptosis by caspase-3 activation in prostate cancer cells, which suggest its use as a potential therapeutic target in the future.

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References

  1. Bartel DP (2004). MicroRNAs: genomics, biogenesis, mechanism, and function. Cell, 116, 281-97. https://doi.org/10.1016/S0092-8674(04)00045-5
  2. Calin GA, Croce CM (2006). MicroRNA signatures in human cancers. Nat Rev Cancer, 6, 857-66. https://doi.org/10.1038/nrc1997
  3. Filipowicz W, Bhattacharyya SN, Sonenberg N (2008). Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nat Rev Genet, 9, 102-14.
  4. Guo ST, Jiang CC, Wang GP, et al (2013). MicroRNA-497 targets insulin-like growth factor 1 receptor and has a tumour suppressive role in human colorectal cancer. Oncogene, 32, 1910-20. https://doi.org/10.1038/onc.2012.214
  5. Hagman Z, Larne O, Edsjo A, et al (2010). miR-34c is downregulated in prostate cancer and exerts tumor suppressive functions. Int J Cancer, 127, 2768-76. https://doi.org/10.1002/ijc.25269
  6. He L, Hannon GJ (2004). MicroRNAs: small RNAs with a big role in gene regulation. Nat Rev Genet, 5, 522-31. https://doi.org/10.1038/nrg1379
  7. Hsieh IS, Chang KC, Tsai YT, et al (2013). MicroRNA-320 suppresses the stem cell-like characteristics of prostate cancer cells by down-regulating the Wnt/beta-catenin signaling pathway. Carcinogenesis, 34, 530-8. https://doi.org/10.1093/carcin/bgs371
  8. Kefas B, Godlewski J, Comeau L, et al (2008). microRNA-7 inhibits the epidermal growth factor receptor and the Akt pathway and is down-regulated in glioblastoma. Cancer Res, 68, 3566-72. https://doi.org/10.1158/0008-5472.CAN-07-6639
  9. Li D, Zhao Y, Liu C, et al (2011). Analysis of MiR-195 and MiR-497 expression, regulation and role in breast cancer. Clin Cancer Res, 17, 1722-30. https://doi.org/10.1158/1078-0432.CCR-10-1800
  10. Linsley PS, Schelter J, Burchard J, et al (2007). Transcripts targeted by the microRNA-16 family cooperatively regulate cell cycle progression. Mol Cell Biol, 27, 2240-52. https://doi.org/10.1128/MCB.02005-06
  11. Lu J, Getz G, Miska EA, et al (2005). MicroRNA expression profiles classify human cancers. Nature, 435, 834-8. https://doi.org/10.1038/nature03702
  12. Luo M, Shen D, Zhou X, Chen X, Wang W (2013). MicroRNA-497 is potential prognostic marker in human cervical cancer and functions as a tumor suppressor by targeting the insulin-like growth factor 1 receptor. Surgery, 12, 751-9.
  13. Porkka KP, Pfeiffer MJ, Waltering KK, et al (2007). MicroRNA expression profiling in prostate cancer. Cancer Res, 67, 6130-5.. https://doi.org/10.1158/0008-5472.CAN-07-0533
  14. Ribas J, Ni X, Haffner M, et al (2009). miR-21: an androgen receptor-regulated microRNA that promotes hormonedependent and hormone-independent prostate cancer growth. Cancer Res, 69, 7165-9. https://doi.org/10.1158/0008-5472.CAN-09-1448
  15. Ru P, Steele R, Newhall P, et al (2012). miRNA-29b suppresses prostate cancer metastasis by regulating epithelialmesenchymal transition signaling. Mol Cancer Ther, 11, 1166-73. https://doi.org/10.1158/1535-7163.MCT-12-0100
  16. Shen L, Li J, Xu, L, et al (2012). miR-497 induces apoptosis of breast cancer cells by targeting Bcl-w. Exp Ther Med, 3, 475-80.
  17. Siegel R, Naishadham, D, Jemal A (2012). Cancer statistics. CA Cancer J Clin, 62, 10-29. https://doi.org/10.3322/caac.20138
  18. Sun H, Li QW, Lv XY, et al (2010). MicroRNA-17 posttranscriptionally regulates polycystic kidney disease-2 gene and promotes cell proliferation. Mol Biol Rep, 37, 2951-8. https://doi.org/10.1007/s11033-009-9861-3
  19. Vasudevan S, Tong Y, Steitz JA (2007). Switching from repression to activation: microRNAs can up-regulate translation. Science, 318, 1931-4. https://doi.org/10.1126/science.1149460
  20. Watahiki A, Wang Y, Morris J, et al (2011). MicroRNAs associated with metastatic prostate cancer. PLoS One, 6, e24950. https://doi.org/10.1371/journal.pone.0024950
  21. Yadav S, Pandey A, Shukla A, et al (2011). miR-497 and miR-302b regulate ethanol-induced neuronal cell death through BCL2 protein and cyclin D2. J Biol Chem, 286, 37347-57. https://doi.org/10.1074/jbc.M111.235531
  22. BCL2 protein and cyclin D2. J Biol Chem, 286, 37347-57. Zhang J, Yang Y, Yang T, et al (2010). microRNA-22, downregulated in hepatocellular carcinoma and correlated with prognosis, suppresses cell proliferation and tumourigenicity. Br J Cancer, 103, 1215-20. https://doi.org/10.1038/sj.bjc.6605895
  23. Zheng D, Radziszewska A, Woo P (2012). MicroRNA 497 modulates interleukin 1 signalling via the MAPK/ERK pathway. FEBS Lett, 586, 4165-72. https://doi.org/10.1016/j.febslet.2012.10.014
  24. Zhu W, Zhu D, Lu S, et al (2012). miR-497 modulates multidrug resistance of human cancer cell lines by targeting BCL2. Med Oncol, 29, 384-91. https://doi.org/10.1007/s12032-010-9797-4

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