대한수의학회지 (Korean Journal of Veterinary Research)

  • 제53권3호
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  • Pages.159-162
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  • 2013
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  • 2466-1384(pISSN)
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  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지
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Immunohistochemical localization of galectin-3 in the brain with Theiler's murine encephalomyelitis virus (DA strain) infection

  • Shin, Taekyun (Department of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University) ;
  • Carrillo-Salinas, Francisco J. (Department of Functional and Systems Neurobiology, Neuroimmunology Group, Instituto Cajal, CSIC) ;
  • Martinez, Ana Feliu (Department of Functional and Systems Neurobiology, Neuroimmunology Group, Instituto Cajal, CSIC) ;
  • Mecha, Miriam (Department of Functional and Systems Neurobiology, Neuroimmunology Group, Instituto Cajal, CSIC) ;
  • Guaza, Carmen (Department of Functional and Systems Neurobiology, Neuroimmunology Group, Instituto Cajal, CSIC)
  • 투고 : 2013.05.16
  • 심사 : 2013.07.15
  • 발행 : 2013.09.30
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초록

Galectin-3 is a ${\beta}$-galactoside-binding lectin that plays a role in neuroinflammation through cell migration, proliferation, and apoptosis. In the present study, regulation of galectin-3 was examined in the brain of mice infected with the Daniel strain of Theiler's murine encephalomyelitis virus (TMEV) at days 7 and 81 post-infection by immunohistochemistry. Immunohistochemistry revealed that galectin-3 was mainly localized in ionized calcium-binding adapter 1-positive macrophages/activated microglia, but not in Iba-1-positive ramified microglia. Galectin-3 was also weakly detected in some astrocytes in the same encephalitic lesions, but not in neurons and oligodendrocytes. Collectively, the present findings suggest that galectin-3, mainly produced by activated microglia/macrophages, may be involved in the pathogenesis of virus induced acute inflammation in the early stage as well as the chronic demyelinating lesions in Daniel strain of TMEV induced demyelination model.

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참고문헌

  1. Almkvist J, Karlsson A. Galectins as inflammatory mediators. Glycoconj J 2004, 19, 575-581.
  2. Arévalo-Martín Á, Vela JM, Molina-Holgado E, Borrell J, Guaza C. Therapeutic action of cannabinoids in a murine model of multiple sclerosis. J Neurosci 2003, 23, 2511-2516.
  3. Comte I, Kim Y, Young CC, van der Harg JM, Hockberger P, Bolam PJ, Poirier F, Szele FG. Galectin-3 maintains cell motility from the subventricular zone to the olfactory bulb. J Cell Sci 2011, 124, 2438-2447. https://doi.org/10.1242/jcs.079954
  4. Correa F, Hernangomez-Herrero M, Mestre L, Loria F, Docagne F, Guaza C. The endocannabinoid anandamide downregulates IL-23 and IL-12 subunits in a viral model of multiple sclerosis: evidence for a cross-talk between IL- 12p70/IL-23 axis and IL-10 in microglial cells. Brain Behav Immun 2011, 25, 736-749. https://doi.org/10.1016/j.bbi.2011.01.020
  5. Dal Canto MC, Lipton HL. Multiple sclerosis. Animal model: Theiler's virus infection in mice. Am J Pathol 1977, 88, 497-500.
  6. De Giusti CJ, Alberdi L, Frik J, Ferrer MF, Scharrig E, Schattner M, Gomez RM. Galectin-3 is upregulated in activated glia during Junin virus-induced murine encephalitis. Neurosci Lett 2011, 501, 163-166. https://doi.org/10.1016/j.neulet.2011.07.007
  7. Dumic J, Dabelic S, Flögel M. Galectin-3: an open-ended story. Biochim Biophys Acta 2006, 1760, 616-635. https://doi.org/10.1016/j.bbagen.2005.12.020
  8. Jiang HR, Al Rasebi Z, Mensah-Brown E, Shahin A, Xu D, Goodyear CS, Fukada SY, Liu FT, Liew FY, Lukic ML. Galectin-3 deficiency reduces the severity of experimental autoimmune encephalomyelitis. J Immunol 2009, 182, 1167-1173. https://doi.org/10.4049/jimmunol.182.2.1167
  9. Kim H, Lee J, Hyun JW, Park JW, Joo H, Shin T. Expression and immunohistochemical localization of galectin-3 in various mouse tissues. Cell Biol Int 2007, 31, 655-662. https://doi.org/10.1016/j.cellbi.2006.11.036
  10. King RD, Lubinski JM, Friedman HM. Herpes simplex virus type 1 infection increases the carbohydrate binding activity and the secretion of cellular galectin-3. Arch Virol 2009, 154, 609-618. https://doi.org/10.1007/s00705-009-0351-7
  11. Kummerfeld M, Seehusen F, Klein S, Ulrich R, Kreutzer R, Gerhauser I, Herder V, Baumgärtner W, Beineke A. Periventricular demyelination and axonal pathology is associated with subependymal virus spread in a murine model for multiple sclerosis. Intervirology 2012 55, 401-416. https://doi.org/10.1159/000336563
  12. Lalancette-Hébert M, Swarup V, Beaulieu JM, Bohacek I, Abdelhamid E, Weng YC, Sato S, Kriz J. Galectin-3 is required for resident microglia activation and proliferation in response to ischemic injury. J Neurosci 2012, 32, 10383-10395. https://doi.org/10.1523/JNEUROSCI.1498-12.2012
  13. Mecha M, Carrillo-Salinas FJ, Mestre L, Feliú A, Guaza C. Viral models of multiple sclerosis: neurodegeneration and demyelination in mice infected with Theiler's virus. Prog Neurobiol 2013, 101-102, 46-64. https://doi.org/10.1016/j.pneurobio.2012.11.003
  14. Mestre L, Docagne F, Correa F, Loria F, Hernangomez M, Borrell J, Guaza C. A cannabinoid agonist interferes with the progression of a chronic model of multiple sclerosis by downregulating adhesion molecules. Mol Cell Neurosci 2009, 40, 258-266. https://doi.org/10.1016/j.mcn.2008.10.015
  15. Molina-Holgado E, Arevalo-Martin A, Vela JM, Guaza C. Theiler's virus encephalomyelitis infection as a model for multiple sclerosis: cytokines and pathogenic mechanisms. Rev Neurol 2002, 35, 973-978.
  16. Pajoohesh-Ganji A, Knoblach SM, Faden AI, Byrnes KR. Characterization of inflammatory gene expression and galectin-3 function after spinal cord injury in mice. Brain Res 2012, 1475, 96-105. https://doi.org/10.1016/j.brainres.2012.07.058
  17. Pasquini LA, Millet V, Hoyos HC, Giannoni JP, Croci DO, Marder M, Liu FT, Rabinovich GA, Pasquini JM. Galectin-3 drives oligodendrocyte differentiation to control myelin integrity and function. Cell Death Differ 2011, 18, 1746-1756. https://doi.org/10.1038/cdd.2011.40
  18. Reichert F, Rotshenker S. Deficient activation of microglia during optic nerve degeneration. J Neuroimmunol 1996, 70, 153-161. https://doi.org/10.1016/S0165-5728(96)00112-9
  19. Reichert F, Rotshenker S. Galectin-3/MAC-2 in experimental allergic encephalomyelitis. Exp Neurol 1999, 160, 508-514. https://doi.org/10.1006/exnr.1999.7229
  20. Rotshenker S. The role of Galectin-3/MAC-2 in the activation of the innate-immune function of phagocytosis in microglia in injury and disease. J Mol Neurosci 2009, 39, 99-103. https://doi.org/10.1007/s12031-009-9186-7
  21. Sato F, Tanaka H, Hasanovic F, Tsunoda I. Theiler's virus infection: pathophysiology of demyelination and neurodegeneration. Pathophysiology 2011, 18, 31-41. https://doi.org/10.1016/j.pathophys.2010.04.011
  22. Shin T. The pleiotropic effects of galectin-3 in neuroinflammation: a review. Acta Histochem 2013, 115, 407-411. https://doi.org/10.1016/j.acthis.2012.11.010
  23. Sundblad V, Croci DO, Rabinovich GA. Regulated expression of galectin-3, a multifunctional glycan-binding protein, in haematopoietic and non-haematopoietic tissues. Histol Histopathol 2011, 26, 247-265.
  24. Takasaki I, Taniguchi K, Komatsu F, Sasaki A, Andoh T, Nojima H, Shiraki K, Hsu DK, Liu FT, Kato I, Hiraga K, Kuraishi Y. Contribution of spinal galectin-3 to acute herpetic allodynia in mice. Pain 2012, 153, 585-592. https://doi.org/10.1016/j.pain.2011.11.022