대한수의학회지 (Korean Journal of Veterinary Research)

  • 제53권2호
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  • Pages.95-101
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  • 2013
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  • 2466-1384(pISSN)
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  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Salmonella enterica serovars Enteritidis, Gallinarum 및 Typhimurium의 마우스 면역반응의 비교평가

Comparative evaluation of the murine immune responses to Salmonella enterica serovars Enteritidis, Gallinarum and Typhimurium infection

  • 김기주 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 김두리 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 선지선 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 박소연 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 조영재 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 고현정 (강원대학교 약학대학) ;
  • 주홍구 (제주대학교 수의과대학) ;
  • 한태욱 (강원대학교 수의과대학 및 동물의학종합연구소)
  • Kim, Kiju (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Kim, Dooree (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Sun, Jisun (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Park, Soyeon (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Cho, Youngjae (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Ko, Hyun-Jeong (College of Pharmacy, Kangwon National University) ;
  • Joo, Hong-Gu (College of Veterinary Medicine, Jeju National University) ;
  • Hahn, Tae-Wook (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
  • 투고 : 2013.02.28
  • 심사 : 2013.05.15
  • 발행 : 2013.06.30
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초록

The study was carried out to evaluate and compare the immune responses in mice experimentally infected with either wild-type or isogenic mutants of Salmonella enterica serovars Enteritidis (SE), Salmonella Typhimurium (ST) and Gallinarum (SG). The mutant strains were constructed by allelic replacement of some virulence-associated genes in the wild-type strains. Seven-week-old female BALB/c mice were orally or intraperitoneally inoculated by injecting bacterial suspension. To evaluate the immune responses, enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot (ELISPOT) assay were conducted with serum and fecal samples. As a result, the mice group infected orally with the SE mutant strain showed the highest level of specific IgA-secreting splenocytes, compared to the other groups. The peritoneally injected groups showed the greater levels of IgG1 than the orally injected groups, which was in a good agreement with the previous studies. In addition, the mutant infected groups had the similar secretion levels of antibodies with the wild-type infected groups. These results demonstrated that the SE mutant strain elicited humoral immune response as much as wild-type, implying that it can be useful as a delivery vehicle as well as a candidate of a live attenuated vaccine.

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