Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of the Antimicrobial Peptide $\small{D}$-Nal-Pac-525 on the Growth of Streptococcus mutans and Its Biofilm Formation

  • Li, Huajun (Department of Biochemistry and Molecular Biology, Dalian Medical University) ;
  • Cheng, Jya-Wei (Institute of Biotechnology and Department of Medical Science, National Tsing Hua University) ;
  • Yu, Hui-Yuan (Institute of Biotechnology and Department of Medical Science, National Tsing Hua University) ;
  • Xin, Yi (Department of Biotechnology, Dalian Medical University) ;
  • Tang, Li (Department of Microecology, Dalian Medical University) ;
  • Ma, Yufang (Department of Biochemistry and Molecular Biology, Dalian Medical University)
  • Received : 2013.12.14
  • Accepted : 2013.04.23
  • Published : 2013.08.28
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Abstract

Streptococcus mutans is the primary etiological agent of dental caries. The antimicrobial peptide $\small{D}$-Nal-Pac-525 was designed by replacing the tryptophans of the Trp-rich peptide Pac-525 with $\small{D}$-${\beta}$-naphthyalanines. To assess the effect of $\small{D}$-Nal-Pac-525 on cariogenic bacteria, the activity of $\small{D}$-Nal-Pac-525 on the growth of S. mutans and its biofilm formation were examined. $\small{D}$-Nal-Pac-525 showed robust antimicrobial activity against S. mutans (minimum inhibitory concentration of 4 ${\mu}g/ml$). Using scanning electron microscopy and transmission electron microscopy, it was shown that $\small{D}$-Nal-Pac-525 caused morphological changes and damaged the cell membrane of S. mutans. $\small{D}$-Nal-Pac-525 inhibited biofilm formation of S. mutans at 2 ${\mu}g/ml$. The results of this study suggest that $\small{D}$-Nal-Pac-525 has great potential for clinical application as a dental caries-preventing agent.

Keywords

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