Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Repression of Type-1 Fimbriae in Shiga Toxin-Producing Escherichia coli O91:H21 Isolated from Asymptomatic Human Carriers in Korea

  • Kim, Jung-Beom (Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment) ;
  • Oh, Kyung-Hwan (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health) ;
  • Park, Mi-Sun (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health) ;
  • Cho, Seung-Hak (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health)
  • Received : 2012.11.28
  • Accepted : 2013.01.18
  • Published : 2013.05.28
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Abstract

Seventy-four Shiga toxin-producing Escherichia coli (STEC) isolates belonging to the serotype O91:H21 were isolated from 1,643 asymptomatic human carriers in a STEC outbreak at Gwangju in Korea. Although the isolates did not cause any symptoms, all of them produced Shiga toxins 1 (Stx1) and 2 (Stx2). In order to determine why these strains cause no symptoms, we explored the differences in virulence potential between the asymptomatic STEC O91:H21 isolates and symptomatic STEC O91:H21 strains (ATCC 51435 and ATCC 51434). The asymptomatic STEC O91:H21 isolates showed strongly reduced cytopathic effects compared with the symptomatic strains when intact bacterial cells were used as an inoculant. Moreover, we found a reduced adherence phenotype when testing asymptomatic strains on HeLa cells. Real-time quantitative PCR results suggest that transcriptional repression of the genes encoding type-1 fimbriae occurs in the asymptomatic isolates but not in the symptomatic strains.

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References

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