DOI QR코드

DOI QR Code

Selective Inhibition of Ammonia Oxidation and Nitrite Oxidation Linked to $N_2O$ Emission with Activated Sludge and Enriched Nitrifiers

  • Ali, Toor Umair (Department of Environmental Sciences and Biotechnology, Hallym University) ;
  • Kim, Minwook (Department of Environmental Sciences and Biotechnology, Hallym University) ;
  • Kim, Dong-Jin (Department of Environmental Sciences and Biotechnology, Hallym University)
  • 투고 : 2013.02.08
  • 심사 : 2013.03.13
  • 발행 : 2013.05.28

초록

Nitrification in wastewater treatment emits a significant amount of nitrous oxide ($N_2O$), which is one of the major greenhouse gases. However, the actual mechanism or metabolic pathway is still largely unknown. Selective nitrification inhibitors were used to determine the nitrification steps responsible for $N_2O$ emission with activated sludge and enriched nitrifiers. Allylthiourea (86 ${\mu}M$) completely inhibited ammonia oxidation and $N_2O$ emission both in activated sludge and enriched nitrifiers. Sodium azide (24 ${\mu}M$) selectively inhibited nitrite oxidation and it led to more $N_2O$ emission than the control experiment both in activated sludge and enriched nitrifiers. The inhibition tests showed that $N_2O$ emission was mainly related to the activity of ammonia oxidizers in aerobic condition, and the inhibition of ammonia monooxygenase completely blocked $N_2O$ emission. On the other hand, $N_2O$ emission increased significantly as the nitrogen flux from nitrite to nitrate was blocked by the selective inhibition of nitrite oxidation.

키워드

참고문헌

  1. American Public Health Association, American Water Works Associations, Water Environment Federation. 2005. Standard Methods for the Examination of Water & Wastewater, pp. 4-108 - 4-129, 21st Ed. APHA/AWWA/WEF, Washington, DC.
  2. Bedard, C. and R. Knowles. 1989. Physiology, biochemistry and specific inhibitors of $CH_4$, $NH_4^+$, and CO oxidation by methanotrophs and nitrifiers. Microbiol. Rev. 53: 68-84.
  3. Beline, F., J. Martinez, D. C. Chadwick, G. Guiraud, and C. M. Coste. 1999. Factors affecting nitrogen transformation and related nitrous oxide emission from aerobically treated piggery slurry. J. Agric. Eng. Res. 7: 235-243.
  4. Butler, M. D., Y. Y. Wang, E. Cartmell, and T. Stephenson. 2009. Nitrous oxide emissions for early warning of biological nitrification failure in activated sludge. Water Res. 43: 1265-1272. https://doi.org/10.1016/j.watres.2008.12.027
  5. Chandran, K. and B. F. Smets. 2000. Single-step nitrification models erroneously describe batch ammonia oxidation profiles when nitrite oxidation becomes rate limiting. Biotechnol. Bioeng. 68: 396-406. https://doi.org/10.1002/(SICI)1097-0290(20000520)68:4<396::AID-BIT5>3.0.CO;2-S
  6. Ginestet, P., J. Audic, V. Urbain, and J. Block. 1998. Estimation of nitrifying bacterial activities by measuring oxygen uptake in the presence of the metabolic inhibitors allylthiourea and azide. Appl. Environ. Microbiol. 64: 2266-2268.
  7. Hellinga, C., A. A. J. C. Schellen, J. W. Mulder, M. C. M. van Loosdrecht, and J. J. Heijnen. 1998. The SHARON process: An innovative method for nitrogen removal from ammonium-rich waste water. Wat. Sci. Technol. 37: 135-142.
  8. Hooper, A. B. and K. R. Terry. 1979. Hydroxylamine oxidoreductase of Nitrosomonas: Production of nitric oxide from hydroxylamine. Biochim. Biophys. Acta 571: 12-20. https://doi.org/10.1016/0005-2744(79)90220-1
  9. Jensen, M. M., B. Thamdrup, and T. Dalsgaard. 2007. Effects of specific inhibitors on anammox and denitrification in marine sediments. Appl. Environ. Microbiol. 73: 3151-3158. https://doi.org/10.1128/AEM.01898-06
  10. Jian, M., X. Q. Jiang, L. Z. Yang, J. Zhang, Q. Y. Qiao, C. D. He, and S. X. Yin. 2006. Nitrous oxide production in a sequence batch reactor wastewater treatment system using synthetic wastewater. Pedosphere 16: 451-456. https://doi.org/10.1016/S1002-0160(06)60075-1
  11. Kampschreur, M. J., H. Temmink, R. Kleerebezem, M. S. M. Jetten, and M. C. M. van Loosdrecht. 2009. Nitrous oxide emission during wastewater treatment. Water Res. 43: 4093-4103. https://doi.org/10.1016/j.watres.2009.03.001
  12. Kester, R. A., W. de Boer, and H. Laanbroek. 1996. Short exposure to acetylene to distinguish between nitrifier and denitrifier nitrous oxide production in soil and sediment samples. FEMS Microbiol. Ecol. 20: 111-120.
  13. Kim, D. J. and Y. Kim. 2011. Effect of ammonium concentration on the emission of $N_2O$ under oxygen-limited autotrophic wastewater nitrification. J. Microbiol. Biotechnol. 21: 988-994. https://doi.org/10.4014/jmb.1101.01033
  14. Kim, D. J. and Y. Kim. 2013. Effect of aeration on nitrous oxide ($N_2O$) emission from nitrogen-removing sequencing batch reactors. J. Microbiol. Biotechnol. 23: 99-105. https://doi.org/10.4014/jmb.1206.06001
  15. Kuenen, J. G. 2008. Anammox bacteria: From discovery to application. Nat. Rev. Microbiol. 6: 320-326. https://doi.org/10.1038/nrmicro1857
  16. Miller, L. G., M. D. Coutlakis, R. S. Oremland, and B. B. Ward. 1993. Selective inhibition of ammonium oxidation and nitrificationlinked $N_2O$ formation by methyl fluoride and dimethyl ether. Appl. Environ. Microbiol. 59: 2457-2464.
  17. Poth, M., D. D. Focht, and D. Lestingi. 1985. $^{15}N$ kinetic analysis of $N_2O$ production by Nitrosomonas europaea: An examination of nitrifier denitrification. Appl. Environ. Microbiol. 49: 1134-1141.
  18. Tallec, G., J. Garnier, and M. Gousailles. 2006. Nitrogen removal in a wastewater treatment plant through biofilters: Nitrous oxide emissions during nitrification and denitrification. Bioproc. Biosyst. Eng. 26: 323-333.
  19. US EPA. 2006. Global Mitigation of Non-$CO_2$ Greenhouse Gases, pp. III-13-III-22. Washington, DC.
  20. Wrage, N., G. L. Velthof, M. L. van Beusichem, and M. Oenema. 2001. Role of nitrifier denitrification in the production of nitrous oxide. Soil Biol. Biochem. 33: 1723-1732. https://doi.org/10.1016/S0038-0717(01)00096-7

피인용 문헌

  1. The history of aerobic ammonia oxidizers: from the first discoveries to today vol.52, pp.7, 2013, https://doi.org/10.1007/s12275-014-4114-0
  2. Confirmation of co-denitrification in grazed grassland vol.5, pp.None, 2013, https://doi.org/10.1038/srep17361
  3. N2O emission during wastewater nitrification with enriched nitrifying bacteria vol.57, pp.2, 2013, https://doi.org/10.1080/19443994.2014.986830
  4. Biomass aggregation influences NaN3 short-term effects on anammox bacteria activity vol.75, pp.5, 2013, https://doi.org/10.2166/wst.2016.587
  5. The interactive effects of various nitrogen fertiliser formulations applied to urine patches on nitrous oxide emissions in grassland vol.56, pp.1, 2013, https://doi.org/10.1515/ijafr-2017-0006
  6. The interactive effects of various nitrogen fertiliser formulations applied to urine patches on nitrous oxide emissions in grassland vol.56, pp.1, 2013, https://doi.org/10.1515/ijafr-2017-0006
  7. Modeling of Pharmaceutical Biotransformation by Enriched Nitrifying Culture under Different Metabolic Conditions vol.52, pp.5, 2013, https://doi.org/10.1021/acs.est.8b00705
  8. Factors Affecting the Effectiveness of Bioelectrochemical System Applications: Data Synthesis and Meta-Analysis vol.4, pp.3, 2013, https://doi.org/10.3390/batteries4030034
  9. Sodium azide inhibition of microbial activities and impact on sludge floc destabilization vol.244, pp.None, 2013, https://doi.org/10.1016/j.chemosphere.2019.125452
  10. Responses of AOA and AOB activity and DNA/cDNA community structure to allylthiourea exposure in the water level fluctuation zone soil vol.27, pp.13, 2013, https://doi.org/10.1007/s11356-020-07952-9
  11. A Critical Review on Nitrous Oxide Production by Ammonia-Oxidizing Archaea vol.54, pp.15, 2013, https://doi.org/10.1021/acs.est.0c03948
  12. Effect of carrier particle size on enrichment and shift in nitrifier community behaviors for treating increased strength wastewater vol.93, pp.10, 2013, https://doi.org/10.1002/wer.1567