Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Gene Amplifications in Porphyrin Pathway on Heme Biosynthesis in a Recombinant Escherichia coli

  • Lee, Min Ju (Department of Biotechnology, The Catholic University of Korea) ;
  • Kim, Hye-Jung (Department of Biotechnology, The Catholic University of Korea) ;
  • Lee, Joo-Young (Department of Biotechnology, The Catholic University of Korea) ;
  • Kwon, An Sung (iNtRON Biotechnology Inc.) ;
  • Jun, Soo Youn (iNtRON Biotechnology Inc.) ;
  • Kang, Sang Hyeon (iNtRON Biotechnology Inc.) ;
  • Kim, Pil (Department of Biotechnology, The Catholic University of Korea)
  • Received : 2013.02.13
  • Accepted : 2013.02.22
  • Published : 2013.05.28
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Abstract

A recombinant E. coli co-expressing ALA synthase (hemA), NADP-dependent malic enzyme (maeB), and dicarboxylic acid transporter (dctA) was reported to synthesize porphyrin derivatives including iron-containing heme. To enhance the synthesis of bacterial heme, five genes of the porphyrin biosynthetic pathway [pantothenate kinase (coaA), ALA dehydratase (hemB), 1-hydroxymethylbilane synthase (hemC), uroporphyrinogen III synthase (hemD), and uroporphyrinogen III decarboxylase (hemE)] were amplified in the recombinant E. coli co-expressing hemA-maeB-dctA. Pantothenate kinase expression enabled the recombinant E. coli to accumulate intracellular CoA. Intracellular ALA was the most enhanced by uroporphyrinogen III synthase expression, porphobilinogen was the most enhanced by ALA dehydratase expression, uroporphyrin and coproporphyrin were the most enhanced by 1-hydroxymethylbilane synthase expression. The strain co-expressing coaA, hemA, maeB, and dctA produced heme of $0.49{\mu}mol/g$-DCW, which was twice as much from the strain without coaA expression. Further pathway gene amplifications for the porphyrin derivatives are discussed based on the results.

Keywords

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