Journal of Microbiology and Biotechnology

  • 제23권5호
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  • Pages.661-667
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  • 2013
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Isolation and Biochemical Characterization of Bacillus pumilus Lipases from the Antarctic

  • 투고 : 2012.12.18
  • 심사 : 2013.01.18
  • 발행 : 2013.05.28
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초록

Lipase-producing bacterial strains were isolated from Antarctic soil samples using the tricaprylin agar plate method. Seven strains with relatively strong lipase activities were selected. All of them turned out to be Bacillus pumilus strains by the 16S rRNA gene sequence analysis. Their corresponding lipase genes were cloned, sequenced, and compared. Finally, three different Bacillus pumilus lipases (BPL1, BPL2, and BPL3) were chosen. Their amino acid sequence identities were in the range of 92-98% with the previous Bacillus pumilus lipases. Their optimum temperatures and pHs were measured to be $40^{\circ}C$ and pH 9. Lipase BPL1 and lipase BPL2 were stable up to $30^{\circ}C$, whereas lipase BPL3 was stable up to $20^{\circ}C$. Lipase BPL2 was stable within a pH range of 6-10, whereas lipase BPL1 and lipase BPL3 were stable within a pH range of 5-11, showing strong alkaline tolerance. All these lipases exhibited high hydrolytic activity toward p-nitrophenyl caprylate ($C_8$). In addition, lipase BPL1 showed high hydrolytic activity toward tributyrin, whereas lipase BPL2 and lipase BPL3 hydrolyzed tricaprylin and castor oil preferentially. These results demonstrated that the three Antarctic Bacillus lipases were alkaliphilic and had a substrate preference toward short- and medium-chain triglycerides. These Antarctic Bacillus lipases might be used in detergent and food industries.

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피인용 문헌

  1. Expression and Biochemical Characterization of Cold-Adapted Lipases from Antarctic Bacillus pumilus Strains vol.23, pp.9, 2013, https://doi.org/10.4014/jmb.1305.05006
  2. Characterization and a point mutational approach of a psychrophilic lipase from an arctic bacterium, Bacillus pumilus vol.36, pp.6, 2013, https://doi.org/10.1007/s10529-014-1475-8
  3. Enhancing Extracellular Lipolytic Enzyme Production In An Arctic Bacterium,Psychrobactersp. ArcL13, By Using Statistical Optimization And Fed-Batch Fermentation vol.45, pp.4, 2015, https://doi.org/10.1080/10826068.2014.940964
  4. Characterizing LipR from Pseudomonas sp. R0-14 and Applying in Enrichment of Polyunsaturated Fatty Acids from Algal Oil vol.25, pp.11, 2013, https://doi.org/10.4014/jmb.1506.06011
  5. Enzymes from Marine Polar Regions and Their Biotechnological Applications vol.17, pp.10, 2013, https://doi.org/10.3390/md17100544