Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Functions of Monocyte Chemotactic Protein-3 in Transgenic Mice Fed a High-Fat, High-Cholesterol Diet

  • An, So Jung (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Jung, Un Ju (Department of Nutrition and Food Science, Kyungpook National University) ;
  • Choi, Myung-Sook (Department of Nutrition and Food Science, Kyungpook National University) ;
  • Chae, Chan Kyu (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Oh, Goo Taek (Division of Life and Pharmaceutical Sciences, Ewha Woman's University) ;
  • Park, Yong Bok (School of Life Sciences and Biotechnology, Kyungpook National University)
  • Received : 2012.10.18
  • Accepted : 2012.10.30
  • Published : 2013.03.28
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Abstract

Monocyte chemotactic protein-3 (MCP-3), a chemokine that is in a superfamily of structurally related small chemotactic cytokines involved in leukocyte trafficking, is regarded as a key factor in atherogenesis. In this study, we examined the changes in atherogenic parameters including hepatic lipid accumulation and oxidative balance in MCP- 3-overexpressing transgenic mice (MCP-3 mice) under atherogenic conditions. To induce an extreme atherogenic condition, mice were fed a high-fat, high-cholesterol (HFHC) diet for 12 weeks. The body weight and food intake were not changed by MCP-3 overexpression in the aorta. On a HFHC diet, the MCP-3 mice had higher plasma levels of total cholesterol and a higher atherogenic index compared with wild-type mice, although there were no differences in the plasma HDL-cholesterol and triglyceride levels. Furthermore, an increase in lipid accumulation was observed in the aortas as well as the livers of the HFHC diet-fed MCP-3 mice compared with wild-type mice. The activities of antioxidant enzymes increased in the livers of the HFHC diet-fed MCP-3 mice, whereas supplementation with antioxidants, naringin and hesperidin, reversed the activities of the hepatic antioxidant enzymes in HFHC diet-fed MCP-3 mice, indicating that there might be more oxidative damage to the tissues in the HFHC diet-fed MCP-3 mice leading to progression towards atherosclerosis and hepatic steatosis. Microarray analyses of the aorta revealed atherosclerosis-, PPARs-, lipoprotein receptor, and apolipoprotein-related genes that were affected by the HFHC diet in MCP-3 mice. These findings suggest that aortic MCP-3 overexpression may contribute to the development of atherosclerosis and hepatic steatosis under atherogenic conditions.

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References

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