Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Unanticipated Gene Deletion in the Transgenic Chicken Employing Ovalbumin Promoter for Oviduct Specific Expression

  • Jang, Tae Young (Department of Zootechnical Science, KyungPook National University) ;
  • Koo, Bon Chul (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Kwon, Mo Sun (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Roh, Ji Yeol (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Kim, Teoan (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Park, Young Sik (Department of Zootechnical Science, KyungPook National University)
  • Received : 2013.08.28
  • Accepted : 2013.09.05
  • Published : 2013.09.30
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Abstract

Transgenic chickens have been spotlighted as an highly potent bioreactor for their fecundity, short generation time, and eggs associated with mass production of protein. In this study, we generated transgenic chickens exhibiting oviduct specific expression of human growth hormone fused to human transferrin for oral administration. Gene of the modified growth hormone located at downstream ovalbumin promoter (~3.6 kb) was introduced to stage X blastodermal cell employing retrovirus vector system. Several transgenic chickens were successfully generated. However, genomic analyses showed unexpected deletion within the transgene. The modification of the transgene seemed to occur during germ cell formation because the deletion was detected only from the sperm DNA of the G0 founder animal. There was no evidence of deletion in the somatic cell DNA samples of the same chicken. Consequently, same pattern of the deletion was confirmed in both somatic and germ cells of the G1 progeny.

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References

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