Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

The Protective Effects of the Ethyl Acetate Fraction and Flavonoids from Taraxacum coreanum against Oxidative Stress in Neuronal Cells Induced by Hydrogen Peroxide and Amyloid Beta

흰민들레 Ethyl Acetate 분획물 및 Flavonoid 화합물의 Hydrogen Peroxide와 Amyloid Beta에 의한 신경세포의 산화적 스트레스 보호 효과

  • Lee, Ah Young (Department of Food Science and Nutrition, Pusan National University) ;
  • Choi, Ji Myung (Department of Food Science and Nutrition, Pusan National University) ;
  • Lee, Sullim (Department of Integrative Plant Science, Chung-Ang University) ;
  • Kim, Hyun Young (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University)
  • 이아영 (부산대학교 식품영양학과) ;
  • 최지명 (부산대학교 식품영양학과) ;
  • 이설림 (중앙대학교 식물시스템과학과) ;
  • 김현영 (경남과학기술대학교 식품과학부) ;
  • 이상현 (중앙대학교 식물시스템과학과) ;
  • 조은주 (부산대학교 식품영양학과)
  • Received : 2013.08.19
  • Accepted : 2013.09.10
  • Published : 2013.09.30
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Abstract

The protective role against oxidative stress under cellular system using C6 glioma cells was studied using the ethyl acetate (EtOAc) fraction, luteolin (1), and luteolin-7-glucoside (2) of Taraxacum coreanum. C6 glioma cells showed low cell viability and high generation of reactive oxygen species (ROS) by the treatment with generator of hydrogen peroxide ($H_2O_2$) and amyloid beta ($A{\beta}_{25-35}$). However, the treatment of the EtOAc fraction attenuated the cellular oxidative stress, resulting in significant elevation of cell viability. In addition, the production of ROS formation was also decreased by the treatment of the EtOAc fraction. Compounds 1 and 2 were isolated from the EtOAc fraction, and the protective effect was evaluated. Compounds 1 and 2 led to the increase of cell viability and decrease of production of ROS against oxidative stress by $H_2O_2$ and $A{\beta}_{25-35}$. The present study indicated that the EtOAc fraction, compounds 1 and 2 from T. coreanum demonstrated protective effects against oxidative stress, suggesting the preventive role against neurodegenerative diseases.

Keywords

References

  1. Forman, H. J., Dorio, R. J. and Skelton, D. C. (1987) Hydroperoxide- induced damage to alveolar macrophage function and membrane integrity. Arch. Biochem. Biophys. 259: 457-465. https://doi.org/10.1016/0003-9861(87)90512-1
  2. Scandalios J. G. (1993) Oxygen stress and superoxide dismutases. Plant Physiol. 101: 7-12.
  3. Reed, T. T. (2011) Lipid peroxidation and neurodegenerative disease. Free Radic. Biol. Med. 51: 1302-1319. https://doi.org/10.1016/j.freeradbiomed.2011.06.027
  4. Zhao, B. (2009) Natural antioxidants protect neurons in Alzheimer's disease and Parkinson's disease. Neurochem. Res. 34: 630-638. https://doi.org/10.1007/s11064-008-9900-9
  5. Kim, J. H., Jeong, C. H., Choi, G. N., Kwak, J. H., Choi, S. G., and Heo, H. J. (2009) Antioxidant and neuronal cell protective effects of methanol extract from Schizandra chinensis using an in vitro system. Korean J. Food Sci. Technol. 41: 712-716.
  6. Chon, S. U. (2012) Antioxidant activity and cytotoxicity of different Taraxacum species in Korea. Korean J. Crop Sci. 57: 51-59. https://doi.org/10.7740/kjcs.2012.57.1.051
  7. Sun, Y. L., Kim, J. H., and Hong, S. K. (2011) Establishment of cell suspension cultures and plant regeneration in white dandelion (Taraxacum coreanum NAKAI). Korean J. Plant Res. 24: 280-285. https://doi.org/10.7732/kjpr.2011.24.3.280
  8. Koo, H. N., Hong, S. H., Song, B. K., Kim, C. H., Yoo, Y. H., and Kim, H. M. (2004) Taraxacum offcinale induces cytotoxicity through TNF-$\alpha$ and IL-$1{\alpha}$ secretion in Hep G2 cells. Life Sci. 74: 1149-1157. https://doi.org/10.1016/j.lfs.2003.07.030
  9. Im, D. Y. and Lee, K. I. (2011) Antioxidant and antibacterial activity and tyrosinase inhibitory activity of the extract and fractions from Taraxacum coreanum Nakai. Korean J. Medicinal Crop Sci. 19: 238-245. https://doi.org/10.7783/KJMCS.2011.19.4.238
  10. Mok, S. Y., Lee, S., Kim, H. M., Lee, J. M., Lee, D. G., Ahn, Y. H., Park, C. G., Cho, E. J., and Lee S. (2011) Inhibition of rat lense aldose reductase by flavonoids from dandelion. Nat. Prod. Sci. 17: 130-134.
  11. Lee, D. S., Li, B., Lee, Y. J., Chun, H. J., Ryu, I. H., Jee, Y. J., Chae, G. U., Kwon, Y. S., Shon, J. U., Kang, H. G., Lee, S. H., An, R. B. and Lee, H. S. (2011) Anti-inflammatory effect of extracts from the roots of Taraxacum coreanum on lipopolysacchaide-induced inflammation in BV2 cells. Kor. J. Oral Maxillofac. Pathol. 35: 231-238.
  12. Lee, S., Choi, M. J., Choi, J. M., Lee, S., Kim, H. Y. and Cho, E. J. (2012) Flavonoids from Taraxacum coreanum protect from radical-induced oxidative damage. J. Med. Plants Res. 6: 5377-5384.
  13. Hu, C. and Kitts, D. D. (2004) Luteolin and luteolin-7-O-glucoside from dandelion flower suppress iNOS and COX-2 in RAW264.7 cells. Mol. Cell. Biochem. 265: 107-113. https://doi.org/10.1023/B:MCBI.0000044364.73144.fe
  14. Lee, S., Han, S., Kim, H. M., Lee, J. M., Mok, S. Y., and Lee, S. (2011) Isolation and identification of phytochemical constituents from Taraxacum coreanum. J. Korean Soc. Appl. Biol. Chem. 54: 73-78.
  15. Zaidi, A., Fernandes, D., Bean, J. L., and Michaelis, M. L. (2009) Effects of paraquat-induced oxidative stress on the neuronal plasma membrane $Ca^{2+}$-ATPase. Free Radic. Biol. Med. 47: 1507-1514. https://doi.org/10.1016/j.freeradbiomed.2009.08.018
  16. Omodeo-Sale, F., Gramigna, D., and Campaniello, R. (1997) Lipid peroxidation and antioxidant systems in rat brain: effect of chronic alcohol consumption. Neurochem. Res. 22: 577-582. https://doi.org/10.1023/A:1022418002765
  17. Pratico, D. (2002) Alzheimer's disease and oxygen radical: new insights. Biochem. Pharmachol. 63: 563-567. https://doi.org/10.1016/S0006-2952(01)00919-4
  18. Hardy, J. and Selkoe, D. J. (2002) The amyloid hypothesis of Alzheimer's disease: progress and problems on the road to therapeutics. Sicence 297: 353-356. https://doi.org/10.1126/science.1072994
  19. Markesbery, W. R. (1997) Oxidative stress hypothesis in Alzheimer's disease. Free Rad. Biol. Med. 23: 134-147. https://doi.org/10.1016/S0891-5849(96)00629-6
  20. Cathcart, R., Schwiers, E., and Ames, B. N. (1983) Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein assay. Anal. Biochem. 134: 111-116.
  21. Butterfield, D. A., Drake, J., Pocernich, C., and Castegna, A. (2001) Evidence of oxidative damage in Alzheimer's disease brain: central role of amyloid beta-peptide. Trends Mol. Med. 7: 548-554. https://doi.org/10.1016/S1471-4914(01)02173-6
  22. Behl, C., Davis, J. B., Lesley, R., and Schubert, D. (1994) Hydrogen peroxide mediates amyloid a protein toxicity. Cell 77: 817-827. https://doi.org/10.1016/0092-8674(94)90131-7
  23. Choi, J. M., Choi, M. J., Lee, S., Yamabe N., Lee S. and Cho, E. J. (2012) Protective effects of ethylacetate fraction from Taraxacum coreanum against peroxynitrite-induced oxidative damage under cellular system. Cancer Prev. Res. 17: 251-256.
  24. Lee, S., Choi, M. J., Choi, J. M., Lee, S., Kim, H. Y. and Cho, E. J. (2012) Flavonoids from Taraxacum coreanum protect from radical-induced oxidative damage. J. Med. Plants Res. 6: 5377-5384.
  25. Sohn, M. K. and Shin, Y. W. (2012) A comparative study of memory improving effects of Taraxaci herba on scopolamine- induced amnesia in mouse. Kor. J. Herbol. 27: 27-35.
  26. Lee, S., Han, S., Kim, H. M., Lee, J. M., Kim, J., Park, C. G., and Lee, S. (2011) Simultaneous determination of luteolin and luteoloside in dandelions using HPLC. Hort. Environ. Biotechnol. 52: 536-540. https://doi.org/10.1007/s13580-011-0214-5