Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Antioxidant Activities of Bromotopsentin from the Marine Sponge Spongosorites sp.

해면으로부터 분리된 Bromotopsentin의 항산화활성

  • Lee, Man Gi (Department of Sports and Leisure Studies, Inje University) ;
  • Kim, Dong-Kyoo (Department of Biomedicinal Chemistry, Inje University)
  • 이만기 (인제대학교 자연과학대학 사회체육학과) ;
  • 김동규 (인제대학교 자연과학대학 의생명화학과)
  • Received : 2013.05.21
  • Accepted : 2013.06.03
  • Published : 2013.09.30
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Abstract

Bromotopsentin (BSM) is a bisindole alkaloid compound, which is recognized as a metabolite of the marine sponge Spongosorites sp. In this study, the antioxidant activity of BSM was investigated. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, the trolox equivalent antioxidant capacity (TEAC) assay, the superoxide radical scavenging (NBT) assay, the lipid peroxidation and hydroxyl radical-induced DNA damage assays were carried out to evaluate the antioxidant activity of BSM. It was found that BSM had stronger scavenging activity on the stable free radical DPPH and superoxide radical than L-ascorbic acid with an $IC_{50}$ value of 62 and 64 ${\mu}M$, respectively. The TEAC value which indicated the total antioxidant capacity of BSM was about 0.8, which was also stronger than L-ascorbic acid. About 1.3 ${\mu}M$ of BSM induced 50% inhibition of lipid peroxidation. 60 nM of BSM exhibited a significant protective activity against DNA strand scission by hydroxyl radical on pBR322 DNA. Taken together, we suggest that BSM possesses strong antioxidant activity, and could be a valuable new addition to the list of anti-aging chemotherapeutic agents.

Keywords

References

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