Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Inhibitory Effects of Resveratrol and Piceid against Pathogens of Rice Plant, and Disease Resistance Assay of Transgenic Rice Plant Transformed with Stilbene Synthase Gene

  • Yu, Sang-Mi (Division of Biotechnology, Chonbuk National University) ;
  • Lee, Ha Kyung (Division of Biotechnology, Chonbuk National University) ;
  • Jeong, Ui-Seon (Division of Biotechnology, Chonbuk National University) ;
  • Baek, So Hyeon (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Noh, Tae-Hwan (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Kwon, Soon Jong (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Yong Hoon (Division of Biotechnology, Chonbuk National University)
  • Received : 2013.06.23
  • Accepted : 2013.07.13
  • Published : 2013.09.30
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Abstract

Resvestrol has been known to inhibit bacterial and fungal growth in vitro, and can be accumulated in plant to concentrations necessary to inhibit microbial pathogens. Hence, stilbene synthase gene has been used to transform to synthesize resveratrol in heterologous plant species to enhance resistance against pathogens. In the present study, we investigated the antimicrobial activities of resveratrol and piceid to bacterial and fungal pathogens, which causing severe damages to rice plants. In addition, disease resistance was compared between transgenic rice varieties, Iksan 515 and Iksan 526 transformed with stlibene synthase gene and non-transgenic rice varieties, Dongjin and Nampyeong. Minimum inhibitory concentration of resveratrol for Burkolderia glumae was 437.5 ${\mu}M$, and the mycelial growth of Biplaris oryzae was slightly inhibited at concentration of 10 ${\mu}M$. However, other bacterial and fungal pathogens are not inhibited by resveratrol and piceid. The expression of the stilbene synthase gene in Iksan 515 and Iksan 526 did not significantly enhanced resistance against bacterial grain rot, bacterial leaf blight, sheath blight, and leaf blight. This study is the first report on the effect of resveratrol and piceid against pathogens of rice plant, and changes of disease resistance of transgenic rice plants transformed with stilbene synthase gene.

Keywords

References

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