Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Morphological Diversity of Mitochondria in Cultured Astrocyte, HeLa, COS7 Cells under High Voltage Electron Microscopy

  • Kim, Hyun-Wook (Department of Anatomy, College of Medicine, Korea University) ;
  • Park, Seung Nam (Department of Anatomy, College of Medicine, Korea University) ;
  • Moon, Younghye (School of Biological Sciences, College of Natural Sciences, Seoul National University) ;
  • Oh, Seung Hak (Department of Anatomy, College of Medicine, Korea University) ;
  • Rhyu, Im Joo (Department of Anatomy, College of Medicine, Korea University)
  • Received : 2013.09.24
  • Accepted : 2013.09.26
  • Published : 2013.09.30
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Abstract

Mitochondrion is an important intracellular organelle controlling energy production essential for cell survival. In addition, it is closely related to cellular apoptosis and necrosis. Linear, branched, circular, and ball-shaped mitochondria have been reported. Recent research suggests that mitochondrial morphology may reflect functional status of the cell. In this study, we investigated the density and ratio of the each morphological categories of mitochondria in a few normal cultured cells; astrocyte, HeLa and COS7 cells, of which metabolic activities are different, with high voltage electron microscopy. The absolute number and relative number per unit area of mitochondria was largest in astrocyte. But, the proportion of different mitochondrial shape was similar among cells. These results shows the numerical profiles but not morphological profiles of mitochondria are related to the metabolic activity of each cell line.

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