생명과학회지 (Journal of Life Science)

  • 제23권9호
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  • Pages.1155-1162
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  • 2013
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
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DOI QR Code

해조공진단의 에스트로겐 유사활성 및 난소를 절제한 흰쥐의 혈소판 응집과 혈청 내 지질 조성에 미치는 영향

The Effects of Seaweed Gongjindan on Estrogen like Activities, Platelet Aggregation and Serum Lipid Levels in Ovariectomized Rats

  • Jeon, Myeongjeong (Department Food and Nutrition, Silla University) ;
  • Kim, Seoyeon (Department Food and Nutrition, Silla University) ;
  • Kim, Bo-Kyung (Department Food and Nutrition, Silla University) ;
  • Cheon, Jihyeon (Department Food and Nutrition, Silla University) ;
  • Park, Seong-Hwan (Department of Pharmaceutical Engineering, Silla University) ;
  • Oh, Eunjin (Korea Traditional Food & Medicine, Pukyeong National University) ;
  • Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, Silla University) ;
  • Kim, Mihyang (Department Food and Nutrition, Silla University)
  • 투고 : 2013.08.20
  • 심사 : 2013.09.26
  • 발행 : 2013.09.30
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초록

갱년기의 여성에는 여러 폐경 증후들이 나타나는데, 특히 에스트로겐의 감소로 인한 혈중의 지질 조성의 변화 등으로 골다공증 및 심혈관계 질환의 발병율이 높아지게 된다. Estrogen의 감소는 다양한 생리적 변화를 초래하며 특히 심혈관계 질환을 빠르게 진행시킨다고 보고되고 있다. 본 연구에서는 에스트로겐 유사활성이 뛰어난 갈조류를 선정하여 해조공진단을 제조하였으며, 실험동물인 흰쥐의 난소를 절제하여 인위적으로 갱년기를 유도한 후 해조공진단이 혈소판 응집능 및 혈중 지질 함량에 미치는 영향을 검토하였다. 에스트로겐 검출시스템을 사용하여 공진단 및 갈조류 추출물의 에스트로겐 유사활성을 검증한 결과, 다시마 및 곰피 열수 추출물에서는 통계적으로 유의한 에스트로겐 유사활성이 나타났으며, 특히 다시마 열수 추출물에서 가장 높은 활성이 나타났다. 이러한 in vitro 실험 결과를 통해 다시마 추출물을 첨가한 해조공진단을 제조한 후, 갱년기를 유도한 실험동물에 투여하여 혈소판 응집능 및 혈청 내 지질 조성에 미치는 영향을 검토하였다. 총 콜레스테롤 함량은 해조공진단 투여로 인해 OVX-CON군에 비해 감소하는 경향을 나타내었으나 유의적인 결과가 나타나지 않은 반면, 혈청 중 중성지방 함량의 경우, 해조공진단을 투여한 OVX-GJD군은 OVX-CON군과 비교하여 중성지방 함량이 유의적으로 감소하는 결과가 나타났다. 혈청 중 HDL-콜레스테롤 농도는 정상군인 SHAM군에 비해 난소를 절제한 OVX-CON군이 유의적으로 감소하였으나, 해조공진단을 첨가한 사료 섭취군(OVX-GJD)은 OVX-CON군에 비해 유의적으로 증가하는 결과를 나타내었다. 또한, 혈소판 응집 저해능을 검토한 결과에서 OVX-CON군에 비해 OVX-GJD군의 혈소판 응집이 유의적으로 감소하여 저해하는 경향을 나타내었다. 따라서 본 연구를 통해 제조된 해조공진단이 에스트로겐 유사활성을 가지고 있으며, 난소절제로 갱년기를 유도한 실험동물에게 이를 섭취시킨 결과 혈중 지질 개선 및 혈소판 응집 저해 효과가 나타나 갱년기 증상 완화에 활용 될 수 있는 소재로 기대된다.

Estrogen like activities were evaluated using ethanol and hot water extracts of gongjindan (GJD) and 3 types of brown algae by an in vitro detection system. Laminaria japonica and Undaria pinnatifida represented statistically significant (p<0.001) estrogen like activities, while GJD and Ecklonia stolonifera did not. Furthermore, activities of hot water extracts ($500{\mu}g/ml$) of Laminaria japonica and three mixture (Ecklonia stolonifera : Undaria pinnatifida : Laminaria japonica, 1: 1: 1) were stronger than that of $10^{-7}M$ $17{\beta}$-estradiol. These results suggest that Laminaria japonica contain estrogen like compounds. Based on these results, the prepared seaweed GJD were used for animal experiments about the effects on inhibition of platelet aggregation and serum lipid levels in ovariectomized rats. 9-week Old female SD-rats were randomly assigned to 3 groups as sham-operated rats, ovariectomized rats and ovariectomized rats that were treated with GJD. The rats were placed on prescribed diets for 5 weeks following ovariectomy. Total cholesterol and triglyceride contents on serum decreased in the SHAM group compared to the OVX-CON group. 5 weeks feeding of GJD resulted in significant lowering of triglyceride and a decreasing tendency of total cholesterol level. The level of HDL-cholesterol on serum increased significantly by feeding diets containing the GJD. The ability of platelet aggregation of group treated with GJD was less than that of the OVX-CON group. These results suggest that the beneficial effects of seaweed gongjindan may be used to improve menopausal syndrome.

키워드

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