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Changes of Nutrient Composition and Antioxidative Activities of Fermented Tea during Fermentation

후발효차의 발효기간별 영양성분 및 항산화 활성의 변화

  • Lee, Kyung-Haeng (Dept. of Food and Nutrition, Korea National University of Transportation) ;
  • Kim, Yong-Shik (Dept. of Food Science and Technology, Korea National University of Transportation) ;
  • Lee, Jong-Yeol (Dept. of Food Science and Technology, Korea National University of Transportation)
  • 이경행 (한국교통대학교 식품영양학과) ;
  • 김용식 (한국교통대학교 식품공학과) ;
  • 이종열 (한국교통대학교 식품공학과)
  • Received : 2013.07.19
  • Accepted : 2013.08.21
  • Published : 2013.09.30

Abstract

To manufacture the fermented tea with hygienic quality, green tea was fermented using Bacillus subtilis, Saccharomyces cerevisiae and Lactobacillus bulgaricus and mineral composition, total amino acid content and antioxidative activity changes were evaluated during the fermentation period. Minerals detected in the fermented tea, the Ca, Fe, Zn, Mg and Mn minerals were detected. Ca and Mg are relatively large compared to other mineral content. Total amino acid content of the control was 3.57%, but total amino acid of fermented teas were higher (3.68~3.85%) during fermentation 20 days. Metal chelating activity of control was 55.11%, Metal chelating activity of the fermented tea using B. subtilis was the highest. In reducing power, O.D. value of the control was 2.27, three kinds of fermented tea were lower than that of control. The fermented teas increased lipid peroxidation inhibition compared to the blank test.

위생적인 후발효차를 제조하기 위하여 B. subtilis, S. cerevisiae 및 L. bulgaricus를 녹차에 각각 접종하고, 발효기간별로 발효시키면서 제조한 후발효차의 영양성분 및 항산화 활성을 측정하였다. 후발효차에서 검출된 무기질로는 Ca, Fe, Zn, Mg, Mn으로 5종의 무기질이 검출되었으며, 그 중 Ca와 Mg는 비교적 다른 무기질에 비하여 많은 함량을 나타내었다. 총 아미노산 함량은 대조군은 3.57%이었으며, 발효 20일차에서는 모든 발효차에서 대조군보다는 높은 총 아미노산 함량(3.68~3.85%)을 보였다. 금속이온 제거능에서는 대조군은 55.11%였으며, 세 균주간 금속이온 제거능의 차이는 전반적으로 B. subtilis > L. bulgaricus > S. cerevisiae의 순으로 B. subtilis를 이용한 후발효차 제조시 금속이온 제거능이 가장 높게 나타났다. 환원력은 대조군의 경우 O.D.값이 2.27이었으며, 후발효차에서는 세가지 후발효차 모두 대조군보다는 낮은 환원력을 나타내었다. 후발효차의 지질과산화 억제능은 세 균주로 제조한 후발효차 모두 blank test 결과와 비교할 때 높은 지질 과산화 억제능을 나타내었다.

Keywords

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