Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Dual Coating Improves the Survival of Probiotic Bifidobacterium Strains during Exposure to Simulated Gastro-Intestinal Conditions

위장관내 조건에서 이중코팅 처리 된 프로바이오틱 비피도박테리움의 생존력 향상

  • Received : 2013.06.14
  • Accepted : 2013.09.25
  • Published : 2013.09.30
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Abstract

Probiotics have been reported to benefit human health by modulating immunity, lowering cholesterol, improving lactose tolerance, and preventing some cancer. Once ingested, probiotic microorganisms have to survive harsh conditions such as low pH, protease-rich condition, and bile salts during their passage through the gastro-intestinal (GI) tract colonize and proliferate to exert their probiotic effects. The dual coating technology, by which the bacteria are doubly coated with peptides and polysaccharides in consecutive order, was developed to protect the ingested bacteria from the harsh conditions. The aim of the study was to evaluate the viable stability of a doubly coated blend of four species of Bifidobacterium by comparing its bile/acid resistance and heat viability in vitro with that of the non-coated blend. After challenges with acid, bile salts, heat, and viable cell counts (VVCs) of the dual coated and non-coated blend were determined by cultivation on agar plates or flow cytometric measurement after being stain with the BacLigtht kit$^{TM}$. The results showed that the dual coated blend was much higher resistant to the acidic or bile salt condition than the non-coated blend and heat viability was also higher, indicating that the dual coating can improve the survival of probiotic bacteria during their transit through the GI tract after consumption.

프로바이오틱 박테리아는 면역력 활성 조절, 콜레스테롤 수치 억제, 유당내성 강화, 항종양 활성 등의 다양한 생리활성 기능으로 건강 증진 효과가 있는 것으로 보고되고 있다. 프로바이오틱 박테리아는 일단 섭취하게 되면 위장관을 통과하는 동안 산도가 낮거나 단백질분해 효소가 많은 열악한 환경에서 생존해야 하며 프로바이오틱 효과를 발휘하기 위해 증식해야 한다. 이중 코팅 기술은 펩타이드와 다당류의 이중코팅으로 섭취된 프로바이오틱 박테리아를 열악한 조건으로부터 보호하기 위해 개발되었다. 본 연구에서는 이중코팅 된 4종의 비피도박테리움 혼합물의 생존 안정성을 평가하기 위해 코팅이 되지 않은 비피도박테리움 혼합물과 담즙, 산 저항성 및 열 안정성을 비교 평가하였다. 이중 코팅 된 균주와 코팅이 되지 않은 균주를 산과 담즙 조건 및 $40^{\circ}C$에 노출 시킨 후 한천배지에 배양하여 생존생육 세포수를 측정하였으며, BacLigtht 키트를 이용하여 염색 한 후 유세포 분석기를 이용하여 생균과 사균의 세포수를 평가하였다. 이중코팅 된 균주 혼합물의 경우 코팅이 되지 않은 균주 혼합물 보다 산, 담즙 내성이 더 높았으며, 열 안전성 또한 코팅 되지 않은 균주 혼합물보다 높은 것으로 나타났다. 이 같은 결과들로 이중코팅 기술은 프로바이오틱 박테리아의 안정성 및 섭취 후 위장관 트랙을 통과하는 동안 균주의 생존률을 향상시킬 수 있음을 확인하였다.

Keywords

References

  1. Agrawal, R. 2005. Probiotics: an emerging food supplement with health benefits. Food Biotechnol. 19, 227–246. https://doi.org/10.1080/08905430500316474
  2. Annan, N.T., Borza, A.D., and Truelstrup Hansen, L. 2008. Encapsulation in alginate-coated gelatin microspheres improves survival of the probiotic Bifidobacterium adolescentis 15703T during exposure to simulated gastro-intestinal conditions. Food Res. Int. 41, 184-193. https://doi.org/10.1016/j.foodres.2007.11.001
  3. Burgain, J., Gaiani, C., Linder, M., and Scher, J. 2011. Encapsulation of probiotic living cells: From laboratory scale to industrial applications. J. Food Engineer. 104, 467-483. https://doi.org/10.1016/j.jfoodeng.2010.12.031
  4. Cha, M.K., Chung, M.J., Kim, J.E., Lee, K.O., and HA, N.J. 2011. Comparison of dual coated(DuolacTM) and uncoated lactic acid bacteria from potential probiotics. Biotechnol. Biotechnol. Eq. 25, 2489-2493. https://doi.org/10.5504/bbeq.2011.0052
  5. Charteris, W.P., Kelly, P.M., Morelli, L., and Collins, J.K. 1998. Development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species in the upper human gastrointestinal tract. J. Appl. Microbiol. 84, 759-768. https://doi.org/10.1046/j.1365-2672.1998.00407.x
  6. Clark, P.A. and Martin, J.H. 1994. Selection of bifidobacteria for use as dietary adjuncts in cultured dairy foods: III - Tolerance to simulated bile concentrations of human small intestines. Cultur. Dairy Products J. 29, 18-21.
  7. Dave, R.I. and Shah, N.P. 1996. Evaluation of media for selective enumeration of Streptococcus thermophilus, Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus, and Bifidobacteria. J. Dairy Sci. 79, 1529-1536. https://doi.org/10.3168/jds.S0022-0302(96)76513-X
  8. De Vos, P., Faas, M.M., Spasojevic, M., and Sikkema, J. 2010. Encapsulation for preservation of functionality and targeted delivery of bioactive food components. Int. Dairy J. 20, 292-302. https://doi.org/10.1016/j.idairyj.2009.11.008
  9. FAO/WHO (Food and Agriculture Organization of the United Nations/World Health Organization) 2002. Guidelines for the Evaluation of Probiotics in Food. London, Ontario, Canada. April 30 and May 1, 2002.
  10. Guarner, F. and Malagelada, J.R. 2003. Gut flora in health and disease. Lancet 361, 512–519. https://doi.org/10.1016/S0140-6736(03)12489-0
  11. Jankovic, I., Sybesma, W., Phothirath, P., Ananta, E., and Mercenier, A. 2010. Application of probiotics in food products - challenges and new approaches. Curr. Opin. Biotechnol. 21, 175-181. https://doi.org/10.1016/j.copbio.2010.03.009
  12. Kailasapathy, K. and Chin, J. 2000. Survival and therapeutic potential of probiotic organisms with reference to Lactobacillus acidophilus and Bifidobacterium spp.. Immunol. Cell Biol. 78, 80-88. https://doi.org/10.1046/j.1440-1711.2000.00886.x
  13. Krasaekoopt, W., Bhandari, B., and Deeth, H. 2003. Evaluation of encapsulation techniques of probiotics for yoghurt. Int. Dairy J. 13, 3-13. https://doi.org/10.1016/S0958-6946(02)00155-3
  14. Lankaputhra, W.E.V. and Shah, N.P. 1997. Improving viability of Lactobacillus acidophilus and bifidobacteria in yogurt using two step fermentation and neutralized mix. Food Australia 49, 363–366.
  15. Mortazavian, A.M., Azizi, A., Ehsani, M.R., Razavi, S.H., Mousavi, S.M., Sohrabvandi, S., and Reinheimer, J.A. 2008. Survival of encapsulated probiotic bacteria in Iranian yogurt drink (Doogh) after the product exposure to simulated gastrointestinal conditions. Milchwissenschaft 63, 427–429.
  16. Ouwehand, A.C. and Salminen, S.J. 1998. The health effects of cultured milk products with viable and non-viable bacteria. Int. Dairy J. 8, 749-758. https://doi.org/10.1016/S0958-6946(98)00114-9
  17. Picot, A. and Lacroix, C. 2004. Encapsulation of Bifidobacteria in whey protein-based microcapsules and survival in stimulated gastrointestinal conditions and in yoghurt. Int. Dairy J. 14, 505-515. https://doi.org/10.1016/j.idairyj.2003.10.008
  18. Saarela, M., Alakomi, H.L., Matto, J., Ahonen, A.M., Puhakka, A., and Tynkkynen, S. 2011. Improving the storage stability of Bifidobacterium breve in low pH fruit juice. Int. J. Food Microbiol. 149, 106-110. https://doi.org/10.1016/j.ijfoodmicro.2010.12.002
  19. Sanders, M.E., Gibson, G., Gill, H.S., and Guarner, F. 2007. Probiotics: their potential to impact human health. CAST issue paper No. 36, October 2007.
  20. Shah, N.P. 2000. Probiotic bacteria: Selective enumeration and survival in dairy foods. J. Dairy Sci. 83, 894-907. https://doi.org/10.3168/jds.S0022-0302(00)74953-8
  21. Sohail, A., Turner, M.S., Coombes, A., Bostrom, T., and Bhandari, B. 2011. Survivability of probiotics encapsulated in alginate gel microbeads using a novel impinging aerosols method. Int. J. Food Microbiol. 145, 162-168. https://doi.org/10.1016/j.ijfoodmicro.2010.12.007
  22. Su, R., Zhu, X.L., Fan, D.D., Mi, Y., Yang, C.Y., and Jia, X. 2011. Encapsulation of probiotic Bifidobacterium longum BIOMA 5920 with alginate-human-like collagen and evaluation of survival in simulated gastrointestinal conditions. Int. J. Biol. Macromol. 49, 979-984. https://doi.org/10.1016/j.ijbiomac.2011.08.018
  23. Succi, M., Tremonte, P., Reale, A., Sorrentino, E., Grazia, L., Pacifico, S., and Coppola, R. 2005. Bile salt and acid tolerance of Lactobacillus rhamnosus strains isolated from Parmigiano Reggiano cheese. FEMS Microbiol. Lett. 244, 129-137. https://doi.org/10.1016/j.femsle.2005.01.037
  24. Talwalkar, A. and Kailasapathy, K. 2004. A review of oxygen toxicity in probiotic yogurts: influence on the survival of probiotic bacteria and protective techniques. Compr. Rev. Food Sci. Food Safety 3, 117-124. https://doi.org/10.1111/j.1541-4337.2004.tb00061.x
  25. Truelstrup Hansen, L., Allan-Wojtas, P.M., Jin, Y.L., and Paulson, A.T. 2002. Survival of Ca-alginate microencapsulated Bifidobacterium spp. in milk and simulated gastrointestinal conditions. Food Microbiol. 19, 35-45. https://doi.org/10.1006/fmic.2001.0452
  26. Weichselbaum, E. 2009. Probiotics and health: a review of the evidence. Nutrition Bulletin 34, 340–373. https://doi.org/10.1111/j.1467-3010.2009.01782.x
  27. Weinbreck, F., Bodnár, I., and Marco, M.L. 2010 Can encapsulation lengthen the shelf-life of probiotic bacteria in dry products? Int. J. Food Microbiol. 136, 364-367. https://doi.org/10.1016/j.ijfoodmicro.2009.11.004

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