Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Application of a Lateral Flow Immunoassay to Determine Ampicillin Residues in Muscle Tissue of Olive Flounder (Paralichthys olivaceus)

  • Cha, Chun Nam (Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University) ;
  • Yu, Eun-Ah (Tongyeong National Quarantine Station, Ministry of Health & Welfare) ;
  • Shin, Min Jung (3M Health Care Business Group Laboratory, 3M Korea) ;
  • Park, Eun Kee (Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University) ;
  • Choi, Hyunju (Elderly Life Redesign Institute, Department of Biomedical Laboratory Science, Inje University) ;
  • Kim, Suk (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu Jang (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
  • Received : 2013.05.23
  • Accepted : 2013.06.12
  • Published : 2013.09.30
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Abstract

Antibiotic Detection Kit (Combination I), a lateral flow immunoassay (LFIA) developed for the detection of antibiotic residues in milk, was utilized for the analysis of antibiotic residues in the muscle tissue of olive flounder. After 60-min treatment by dipping in water dosed with ampicillin (200-g/ton water), the residue depletion of ampicillin was investigated in 25 cultured olive flounder (Paralichthys olivaceus). Muscles of fish were sampled on the 1st, 2nd, 3rd, 4th and 5th day after drug treatment. The concentration of ampicillin in the muscle was determined by LFIA. The absorbance ratio of the sample to the control blank (Bs/Bo) was employed as an index to determine the muscle residues in olive flounder. To investigate the recovery rate, standard solutions were added to muscle samples to give final concentrations in the muscle of 4 and 8 ng/ml. The recovery rates of all spiked samples were > 96% of the spiked value. Ampicillin was detected in the muscle of fish treated with the drug until the 2nd day of the withdrawal period. The present study showed that the LFIA can be easily adopted to predict ampicillin residues in tissue of farmed fishes.

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References

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