Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Effects of White Mulberry (Morus alba) Leaves on Blood Vessel Reactivity in Hyperchloesterolemic Rats

  • Choi, Sang-Hoon (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University) ;
  • Park, Kwan Ha (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University)
  • Received : 2012.12.24
  • Accepted : 2013.09.04
  • Published : 2013.09.30
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Abstract

In atherosclerosis, blood vessels become sensitive to vessel-constricting agents leading to reduced control in the event of abrupt blood pressure changes. Mulberry trees (Morus alba L., MA) have been claimed to contain various bioactive principles that could possibly prevent atherosclerosis development caused by high cholesterol consumption. In order to examine whether MA feeding can prevent the sensitization of blood vessels, MA leaves were fed to rats for 8 weeks and pressor responses to vasoconstricting agents were assessed. Animals were pithed before blood pressure assessments to eliminate reflex compensation in vessel responses. Feeding diets containing high levels of cholesterol led to potentiated pressor responses to sympathetic nerve stimulation, or to injection of norepinephrine, phenylephrine, angiotensin II and vasopressin in pithed rats. These potentiated pressor responses were prevented in rats fed MA leaf-containing diets at 2 or 10% levels. It was also examined in anesthetized non-pithed rats whether similar cholestrol-related sensitization and MA prevention could be observed. However, high cholesterol-induced sensitization in pressor responses were not observed, suggesting that destruction of central cardiovascular control by pithing must have revealed the sensitization responses. It was concluded that MA leaves seem to be active in preventing abnormal blood vessel reactivity caused by hypercholesterolemia.

동맥경화 상태에서는 혈관이 수축물질에 예민하게 반응함으로써 혈압의 급격한 변화에 대한 회복능력이 저하한다. 한편 뽕나무 잎에는 고지방식의 섭취로 인해 발생하는 동맥경화증의 발달을 감소시킬 가능성이 있는 여러 물질이 있다고 추정된다. 뽕나무 잎을 백서에 투여하면 고지방식에 의해 유발된 혈관이 예민해지는 현상을 감소시킬 수 있는지 시험하기 위하여 고지방식 및 뽕나무잎을 8주간 투여하고 혈관수축 물질에 대한 혈압의 반응성을 시험하였다. 순환기반사에 따른 혈압반응 감수성차이를 최소화할 목적으로 동물의 중추신경계를 파괴(pithing)하고 시험하였다. 고콜레스테롤을 함유한 식이를 공급한 백서에서 교감신경계 전기자극이나 norepinephrine, phenylephrine, angiotensin II 및 vasopressin 등의 투여로 유발된 혈압상승반응이 증강되었으며, 사료에 뽕잎을 2% 또는 10% 첨가하면 정상적인 혈압상승반응을 보여주었다. 교감신경계를 파괴하지 않은 채 마취만 한 백서에서는 고콜레스테롤에 의한 반응증강이나 뽕잎에 의한 변화를 관찰할 수 없었다. 이 결과로 부터 뽕잎이 동맥경화에서 나타나는 혈관반응성 변화를 방지하는 데 유용한 것으로 판단할 수 있을 것이다.

Keywords

References

  1. Srivastava S., Kapoor R., Thathola, A. and Srivastava, R.P.: Nutritional quality of leaves of some genotypes of mulberry (Morus alba). Int. J. Food Sci. Nutr. 57, 305-313 (2006). https://doi.org/10.1080/09637480600801837
  2. Ercisli, S.: A short review of the fruit germplasm resources of turkey. Genetic Res. Crop Eval. 51, 419-435 (2004). https://doi.org/10.1023/B:GRES.0000023458.60138.79
  3. Arabshahi-Delouee, S. and Urooj, A.: Antioxidant properties of variuos solvent extract of mulberry (Morus indica L.) leaves. Food Chem. 102, 1233-1240 (2007). https://doi.org/10.1016/j.foodchem.2006.07.013
  4. Choi, E.M. and Hwang, J.K.: Effects of Morus alba leaf extract on the production of nitric oxide, prostaglandin E2 and cytokines in RAW2647 macrophages. Fitoterapia 76, 608-613 (2005). https://doi.org/10.1016/j.fitote.2005.05.006
  5. Butt, M. S., Nazir, A., Sultan, M. T. and Schroen, K.: Morus alba L.: nature's functional tonic. Trends Food Sci. Technol. 19, 505-512 (2008). https://doi.org/10.1016/j.tifs.2008.06.002
  6. Park, K.M., You, J.S., Lee, H.Y., Baek, N.I. and Hwang, J.K.: Kuwanon C: an antibacterial agent from the root bark of Morus alba against oral pathogens: J. Ethnopharmacol. 84, 181-185 (2003). https://doi.org/10.1016/S0378-8741(02)00318-5
  7. Sohn, H.Y., Son, K.H., Kwon, C.S., Kwon G.S. and Kang S.S.: Antimicrobial and cytotoxic activity of 18 prenuylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis. Phytomedicine 11, 666-672 (2004). https://doi.org/10.1016/j.phymed.2003.09.005
  8. Cui, L., Na, M.K, Oh, H., Bae, E.Y., Jeong, D.G. and Ryu, S.E.: Protein tyrosine phosphatase 1B inhibitors from Morus root bark. Bioorganic Med. Chem. Lett. 16, 1426-1429 (2006). https://doi.org/10.1016/j.bmcl.2005.11.071
  9. Kofujita, H., Yaguchi, M., Doi, N. and Suzuki, K.: A novel cytotoxic prenylated flavonoid from the root of Morus alba. J. Insect Biotechnol. Sericol. 73, 113-116 (2004).
  10. Enkhmaa, B., Shiwaku, K., Katsube, T., Kitajima, K., Anuurad, E. and Yamasaki, M.: Mulberry (Morus alba L.) leaves and their major flavonol quercetin 3-(6-malonylglucoside) attenuate atheroscleosis lesion development in LDL receptor- deficient mice. J. Nutr. 135, 729-734 (2005).
  11. Katsube, T., Imawaka, N., Kawano, Y., Yamazaki, Y., Shiwaku, K. and Yamane, Y.: Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated base on LDL antioxidant activity. Food Chem. Acta 97, 25-31 (2006). https://doi.org/10.1016/j.foodchem.2005.03.019
  12. Lee, J., Chae, K., Ha, J., Park, B.Y., Lee, H.S. and Jeong, S.: Regulation of obesity and lipid disorders by herbal extracts from Morus alba, Melissa officinalis and Artemisia capillaris in high-fat diet-induced obese mice. J. Ethnopharmacol. 115, 263-270 (2008). https://doi.org/10.1016/j.jep.2007.09.029
  13. Oku, T., Yamada, M., Nakamura, M., Sadamori, N. and Nakamura, S.: Inhibitory effects of extractives from leaves of Morus alba on human and rat small intestinal disaccharidase activity. Brit. J. Nutr. 95, 933-938 (2006). https://doi.org/10.1079/BJN20061746
  14. Nam, S.Y., Yi, H.K., Lee, J.C., Kim, J.C., Song, C.H. and Park, J.W.: Cortex mori extract induces cancer apoptosis through inhibition of microtuble assembly. Arch. Pharmacol. Res. 25, 191-196 (2002). https://doi.org/10.1007/BF02976562
  15. Du, Z.Y. and Woodman, O.L.: The effect of hypercholesterolaemia and atherosclerosis on $\alpha$-adrenoceptor-mediated vasoconstriction in conscious rabbits and rabbit aorta. Eur. J. Pharmacol. 211, 149-156 (1992). https://doi.org/10.1016/0014-2999(92)90522-6
  16. Rosendorff, C., Hoffman, J.I.E., Verrier, E.D., Rouleau, J. and Boerboom, L.E.: Cholesterol potentiates the coronary artery response to norepinephrine in anesthetized and conscious dogs. Cir. Res., 48, 320-329 (1981). https://doi.org/10.1161/01.RES.48.3.320
  17. Heistad, D.D., Armstrong, M.L., Marcus, M.L., Piegors, D.J. and Mark, A.L.: Augumented responses to vasoconstrictor stimuli in hypercholesterolemic and atherosclerotic monkeys. Cir. Res. 54, 711-718 (1984). https://doi.org/10.1161/01.RES.54.6.711
  18. Reeves, P.G., Nielson, F.H. and Fahey, G.C., Jr.: AIN-93 purified diets for laboratory rodents: final report of the American Institite of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr. 123, 1939- 1951 (1993).
  19. Rural Development Administration, Technology on Morus alba Utilization for Medicinal Substances, Research Report AOM 615-617, RDA (1996).
  20. Shipley, R.E. and Tilden, J.H.: A pithed rats preparation suitable for assaying pressor substances. Proc. Soc. Exp. Biol. Med. 64, 453-455 (1947). https://doi.org/10.3181/00379727-64-15828
  21. Gillespie, J.S., MaClaren, A. and Pollock, D.: A method of stimulating different segments of the autonomic outflow from the spinal column to various organs in the pithed cat and rat. Br. J. Pharmacol. 40, 257-267 (1970). https://doi.org/10.1111/j.1476-5381.1970.tb09919.x
  22. Grieve, D.J., Avella, M.A., Elliot, J. and Botham, K.M.: The interaction between oxidized chylomicron remnants and the aorta of rats fed a normocholesterolaemic or hypercholesterolaemic diets. J. Vasc. Res. 37, 265-275 (2000). https://doi.org/10.1159/000025740
  23. Maguire, J.J., Wiley, K.E., Kuc, R.E., Stoneman, V.E.A., Bennett, M.R. and Davenport, A.P.: Endothelin-mediated vasoconstriction in early atherosclerosis is markedly increased in ApoE−/− mouse but prevented by atorvastatin. Exp. Biol. Med. 231, 806-812 (2008).
  24. Hoffman, B.B.: Catecholamines, sympathomimetic drugs, and adrenergic receptors. In Goodman and Gillman's The Pharmacological Basis of Therapeutics, 10th Ed. (Hardman, J.G., Limbird, L.E. and Gillman, A.G. eds.) McGraw Hill, New York, pp. 215-268 (2001).
  25. Park, K.H.: Induction of cardiovascular anaphylaxis and basic pharmacological analysis of involved mediators in pithed rats. Biomolecules Ther. 16, 299-305 (2008). https://doi.org/10.4062/biomolther.2008.16.4.299
  26. Gillespie, J.S. and Muir, T.C.: A method of stimulating the complete sympathetic outflow from the spinal cord to blood vessels in the pithed rat. Br. J. Pharmacol. Chemother. 30, 78- 87 (1967). https://doi.org/10.1111/j.1476-5381.1967.tb02114.x
  27. Fozard, J.R., Mir, A.K and Middlemiss, D.N.: Cardiovascular response to 8-hydroxy-2-(di-n-propylamino) tetralin (8- OH-DPAT) in the rat: site of action and pharmacological analysis. J. Cardiovasc. Pharmacol. 9, 328-347 (1987). https://doi.org/10.1097/00005344-198703000-00010
  28. Alayan, H.H., Kennedy, B.P. and Ziegler, M.G.: The pressor effect of NO synthase inhibition correlates to pre-existing systolic BP in the rat. Autonom. Neurosci. 95, 32-36 (2002). https://doi.org/10.1016/S1566-0702(01)00350-2
  29. Grekin, R.J., Vollmer, A.P. and Sider, R.S.: Pressor effects of portal venous oleate infusion: a proposed mechanism for obesity hypertension. Hypertension 26, 193-138 (1995). https://doi.org/10.1161/01.HYP.26.1.193
  30. Paolisso, G., Manzella, D., Rizzo, M.R., Ragno, F., Barbiert, M., Varricchio, G. and Varricchio, M.: Elevated plasma fatty acid concentrations stimulate the cardiac autonomous nervous system in healthy subjects. Am. J. Clin. Nutr. 72, 723-730 (2000).
  31. Santos, R.A.S., Campagnole-Santos, M.J. and Andrade, S.P.: Angiotensin-(1-7): an update. Regul. Peptides 91, 45-62 (2000). https://doi.org/10.1016/S0167-0115(00)00138-5
  32. Maybauer, M.O., Maybauer, D.M., Enkhbaatar, P. and Traber, D.L.: Physiology of vasopressin receptors. Best Practice Res. Clin. Anesthesiol. 22, 253-263 (2008). https://doi.org/10.1016/j.bpa.2008.03.003
  33. Averill, D.B. and Diz, D.I.: Angiotensin peptides and baroreflex control of sympathetic outflow: pathways and mechanism of the medulla oblongata. Brain Res. Bull. 51, 119-128 (2000). https://doi.org/10.1016/S0361-9230(99)00237-3
  34. Mulvihill, E.E. and Huff, M.W.: Antiatherogenic properties of flavonoids: implication for cardiovascular health. Adv. Vasc. Biol. 22(Suppl. A), 17-21 (2010).
  35. Heinecke, JW.: Oxidants and antioxidants in the pathogenesis of atherosclerosis: implication for the oxidized low density lipoprotein hypothesis. Athrosclerosis 141, 1-15 (1998).
  36. Brown, A.J. and Jessup, W.: Oxysterols and atherosclerosis. Atherosclerosis 142, 1-28 (1999). https://doi.org/10.1016/S0021-9150(98)00196-8
  37. Lee,Y.J., Lee, W.J. and Choi, S.W.: Polyophenolic compounds and biological activity of four different parts of Morus alba and Cudrania tricuspidata. Collection of Research Papers in Natural Sciences, Catholic Uinversity of Daegu 8, 107-117 (2010).