Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Comparative Analysis of Growth and Antioxidant Enzyme Activities from Two Chrysanthemum Varieties, 'ARTI-purple' and 'ARTI-queen' by Chronic Irradiation of Gamma-ray

감마선 완조사에 따른 국화 'ARTI-purple'과 'ARTI-queen'의 생육 및 항산화 효소 활성 비교 분석

  • Sung, Sang Yeop (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Yu-Mi (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Sang Hoon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Ha, Bo-Geun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kang, Si-Yong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jin-Baek (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Dong Sub (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 성상엽 (한국원자력연구원 첨단방사선연구소) ;
  • 이유미 (한국원자력연구원 첨단방사선연구소) ;
  • 김상훈 (한국원자력연구원 첨단방사선연구소) ;
  • 하보근 (한국원자력연구원 첨단방사선연구소) ;
  • 강시용 (한국원자력연구원 첨단방사선연구소) ;
  • 김진백 (한국원자력연구원 첨단방사선연구소) ;
  • 김동섭 (한국원자력연구원 첨단방사선연구소)
  • Received : 2012.11.21
  • Accepted : 2013.03.18
  • Published : 2013.08.31
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Abstract

Two chrysanthemum varieties, 'ARTI-purple' and 'ARTI-queen', were chronically irradiated with doses of 30, 50, 70, and 100 Gy for four weeks in gamma-phytotron, a long term irradiation facility. We investigated the growth, responses of antioxidant enzymes (ascorbate peroxidase, APX; catalase, CAT; peroxidase, POD; superoxidase dismutase, SOD) and malondialdehyde (MDA) contents under different doses of chronic-irradiation. The five plant growth measurements including plant height, number of leaves, internode length, stalk diameter and leaf thickness were investigated immediately after four week irradiation. The plant height (p<0.001), internode length (p<0.01), the number of leaves (p<0.001) and stalk diameter (p<0.05) were significantly decreased an increasing doses of gamma-ray. Among them, especially, the internode length was remarkably decreased showing the RD50 (Reduction Dose 50) at approximately 65 Gy. The antioxidant response after four weeks of recovery period, ascorbate peroxidase (APX) (p<0.01), superoxide dismutase (SOD) (p<0.01) and peroxidase (POD) (p<0.001) were significantly increased with an increasing dose of gamma-ray. And malondialdehyde (MDA) (p<0.01) contents showed the significant increase at the 70 and 100 Gy which means the oxidative stress was lasting for a considerable period. In this study, the 50 Gy irradiation as optimal dose showed higher growth than the $RD_{50}$, it also showed insignificant differences on the antioxidant responses and MDA contents. However, the 100 Gy dose showed lower growth than $RD_{50}$.

국화 'ARTI-purple'과 'ARTI-queen' 두 품종의 발근된 어린 식물체를 감마파이토트론을 이용하여 4주간에 걸쳐 30, 50, 70, 100Gy로 장기간 완조사를 수행하고 감마선 완조사에 따른 생육, 항산화 효소(ascorbate peroxidase, APX; catalase, CAT; peroxidase, POD; superoxidase dismutase, SOD)의 반응 및 malondialdehyde(MDA) 함량의 변화를 조사하였다. 식물의 생육은 초장, 잎의 수, 절간장, 줄기 직경, 잎 두께 등 5개 요소를 4주간의 완조사 후 관찰하였고, 식물체 초장(p<0.001), 잎의 수(p<0.001), 절간장(p<0.01), 줄기 직경(p<0.05)에서 감마선 완조사에 따른 유의적인 감소가 확인되었다. 그리고 그 가운데 절간장은 약 65Gy에서 $RD_{50}$(Reduction Dose 50)을 보이며 가장 높은 감소를 보였다. 4주 회복 기간이 지난 시점에서 항산화 반응을 확인한 결과, APX(p<0.01), SOD(p<0.01), POD(p<0.001)의 활성이 유의적인 증가를 보였고, MDA 함량은 70과 100Gy 조사구에서 유의적인 증가를 보여 산화스트레스가 상당기간 지속됨을 확인할 수 있었다. 본 연구에서 $RD_{50}$의 생육저하를 보이지 않으며, 항산화 효소의 반응 및 MDA 함량이 대조식물과 유의적이 차이를 보이지 않는 최대 선량은 50Gy이었고, 100Gy 수준에서 $RD_{50}$ 이하의 생육을 보이는 것을 확인할 수 있었다.

Keywords

References

  1. Alothman, M., R. Bhat, and A.A. Karim. 2009. Effects of radiation processing on phytochemicals and antioxidants in plant produce. Trends Food Sci. Technol. 20:201-212 https://doi.org/10.1016/j.tifs.2009.02.003
  2. Apel, K. and H. Hirt. 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. 55:373-399. https://doi.org/10.1146/annurev.arplant.55.031903.141701
  3. Becana, M., J.F. Moran, and I. Iturbe-Ormaetxe. 1998. Iron-dependent oxygen free radical generation in plants subjected to environmental stress: toxicity and antioxidant protection. Plant Soil 201:137-147. https://doi.org/10.1023/A:1004375732137
  4. Del Rio, D., A.J. Stewart, and N. Pellegrini. 2005. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr. Metab. Cardiovas. 15:316-328. https://doi.org/10.1016/j.numecd.2005.05.003
  5. Goo, D.H., B.W. Yae, H.S. Song, I.S. Park, B.H. Han, and H.J. Yu. 2003. Color change in chrysanthemum flower by gamma ray irradiation. J. Kor. Soc. Hort. Sci.44:1006-1009.
  6. Heath, R.L. and L. Packer. 1968. Photoperoxidation in isolated chloro-plasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125:180-198.
  7. Hiroyasu, Y.S., Akemi, D. Konosuke, and M. Toshikazu. 2008. Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum. Breed. Sci. 58:331-335. https://doi.org/10.1270/jsbbs.58.331
  8. Hiroyasu, Y., S. Akemi, H. Yoshihiro, D. Konosuke, T. Atsushi, and M. Toshikazu. 2009. Mutation induction with ion beam irradiation of lateral buds of chrysanthemum and analysis of chimeric structure of induced mutants. Euphytica 165:97-103. https://doi.org/10.1007/s10681-008-9767-5
  9. Kim, D.S., J.B. Kim, E.J. Goh, W.J. Kim, S.H. Kim, Y.W. Seo, C.S. Jang, and S.Y. Kang. 2011a. Antioxidant response of arabidopsis plants to gamma irradiation: Genome-wide expression profiling of the ROS scavenging and signal transduction pathways. J. Plant Physiol.168:1960-1971. https://doi.org/10.1016/j.jplph.2011.05.008
  10. Kim, D.S., M.R. Song, S.H. Kim, D.S. Jang, S.Y. Kang, J.B. Kim, S.H. Kim, and B.K. Ha. 2011b. Physiological responses of rice by acute and chronic gamma irradiation. J. Radiat. Industry 5:55-62.
  11. Kim, J.T. 2012. Case study about export of Korean lily to Japan. J. Korea Trade 37(4):31-52.
  12. Kim, Y.H., C. Kim, H.S. Lee, and S.S. Kwak. 2009. Changes in activities of antioxidant enzymes and their gene expression during leaf development of sweetpotato. Plant Growth Regul. 58:235-241. https://doi.org/10.1007/s10725-009-9371-2
  13. Kimura, M. and T. Ohta. 1971. Protein polymorphism as a phase of molecular evolution. Nature 229:467-469. https://doi.org/10.1038/229467a0
  14. Lutts, S., J.M. Kinet, and J. Bouharmont. 1996. NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Ann. Bot. 75:389-398
  15. Misra, P., S.K. Datta, and D. Chakrabarty. 2003. Mutation in flower colour and shape of chrysanthemum morifolium induced by $\gamma$-radiation. Biol. Plant. 47:153-156.
  16. Nagatomi, S. 1991. Flower color mutants derived from floral organ cultures of chronic irradiated plants in chrysanthemum. Technical News. No. 36. Institute of Radiation Breeding, National Institute of Agrobiological Sciences, Nakagun, Japan.
  17. Plaa, G.L. and H. Witschi. 1976. Chemicals, drugs, and lipid peroxidation. Annu. Rev. Pharmacol. Toxicol. 16:125-142. https://doi.org/10.1146/annurev.pa.16.040176.001013
  18. Shirley, B.W., S. Hanley, and H.M. Goodman. 1992. Effects of ionizing radiation on a plant genome: Analysis of two Arabidopsis transparent test a mutations. Plant Cel. 4:333-347. https://doi.org/10.1105/tpc.4.3.333
  19. Sidrak, G.H. and A. Suess. 1973. Effects of low doses of gamma radiation on the growth and yield of two varieties of tomato. Radiat. Bot. 13:309-314. https://doi.org/10.1016/S0033-7560(73)90040-9
  20. Spychalla, J.P. and S.L. Desborough. 1990. Superoxide dismutase, catalase, and $\alpha$-tocopherol content of stored potato tubers. Plant Physiol. 94:1214-1218. https://doi.org/10.1104/pp.94.3.1214
  21. Wang, W.B., Y.H. Kim, H.S. Lee, K.Y. Kim, X.P. Deng, and S.S. Kwak. 2009. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiol. Biochem. 47:570-577. https://doi.org/10.1016/j.plaphy.2009.02.009
  22. Wi, S.G., B.Y. Chung, J.S. Kim, J.H. Kim, M.H. Baek, J.W. Lee, and Y.S. Kim. 2007. Effects of gamma irradiation on morphological changes and biological responses in plants. Micron. 38:553-564. https://doi.org/10.1016/j.micron.2006.11.002
  23. Yamaguchi, H., A. Shimizu, K. Degi, and T. Morishita. 2008. Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum. Breed. Sci. 58:331-335. https://doi.org/10.1270/jsbbs.58.331