Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Differential Expression of Gene Profiles in MRGX-treated Lung Cancer

  • Kwon, Yong-Kyun (East-West Cancer Center, Daejeon University College of Oriental Medicine) ;
  • Lee, Seung-Yeul (Division of Life Science, Korea Basic Science Institute) ;
  • Kang, Hwan-Soo (Department of Life Science, Dongguk University) ;
  • Sung, Jung-Suk (Department of Life Science, Dongguk University) ;
  • Cho, Chong-Kwan (East-West Cancer Center, Daejeon University College of Oriental Medicine) ;
  • Yoo, Hwa-Seung (East-West Cancer Center, Daejeon University College of Oriental Medicine) ;
  • Shin, Seungjin (Department of Chemical and Biological Engineering, Northwestern University) ;
  • Choi, Jong-Soon (Division of Life Science, Korea Basic Science Institute) ;
  • Lee, Yeon-Weol (East-West Cancer Center, Daejeon University College of Oriental Medicine) ;
  • Jang, Ik-Soon (Division of Life Science, Korea Basic Science Institute)
  • Received : 2013.06.15
  • Accepted : 2013.06.18
  • Published : 2013.09.30
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Abstract

Objectives: Modified regular ginseng extract (MRGX) has stronger anti-cancer activity-possessing gensenoside profiles. Methods: To investigate changes in gene expression in the MRGX-treated lung cancer cells (A549), we examined genomic data with cDNA microarray results. After completing the gene-ontology-based analysis, we grouped the genes into up-and down-regulated profiles and into ontology-related regulated genes and proteins through their interaction network. Results: One hundred nine proteins that were up- and down-regulated by MRGX were queried by using IPA. IL8, MMP7 and PLAUR and were found to play a major role in the anti-cancer activity in MRGX-treated lung cancer cells. These results were validated using a Western blot analysis and a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. Conclusions: Most MRGX-responsive genes are up-regulated transiently in A549 cells, but down-regulated in a sustained manner in lung cancer cells.

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References

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