한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제46권4호
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  • Pages.351-358
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  • 2013
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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어류 알로부터 Protease Inhibitors의 크로마토그래피법에 의한 분획

Chromatographic Fractionation of Protease Inhibitors from Fish Eggs

  • 김진수 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 김기현 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 김현정 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 김민지 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 박성환 (경상대학교 식품영양학과/해양산업연구소) ;
  • 이현지 (경상대학교 식품영양학과/해양산업연구소) ;
  • 허민수 (경상대학교 식품영양학과/해양산업연구소)
  • Kim, Jin-Soo (Department of Seafood Science & Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Ki Hyun (Department of Seafood Science & Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Hyeon Jeong (Department of Seafood Science & Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Min Ji (Department of Seafood Science & Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Park, Sung Hwan (Department of Food & Nutrition/Institute of Marine Industry, Gyeongsang National University) ;
  • Lee, Hyun Ji (Department of Food & Nutrition/Institute of Marine Industry, Gyeongsang National University) ;
  • Heu, Min Soo (Department of Food & Nutrition/Institute of Marine Industry, Gyeongsang National University)
  • 투고 : 2013.06.11
  • 심사 : 2013.07.24
  • 발행 : 2013.08.31
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초록

A protease inhibitor from fish eggs was fractionated using chromatographic methods. The fractionation efficiency was evaluated in terms of specific inhibitory activity (SIA, U/mg), purity (fold), total inhibitory activity (TIA, U), and recovery (%). The protease inhibitor (PI) from egg extracts of skipjack tuna (ST Katsuwonus pelamis), yellowfin tuna (YT Thunnus albacares) and Alaska pollock (AP Theragra chalcogramma) was fractionated using Sephadex G-50 gel filtration and DEAE-Sepharose CL-6B anion exchange chromatography based on protein size exclusion and net charge, respectively. Fractions exhibiting strong inhibitory activity were contained in the 30-50 kDa fraction on gel filtration and in the range of 0.4-0.7 M NaCl gradient fraction on anion exchange chromatography. The respective TIA and percent recovery of the fraction obtained with gel filtration toward trypsin and $N{\alpha}$-benzoyl-L-arginine-p-nitroanilide (BAPNA) were 2,758.7 U and 29.6% for ST, 1,005.5 U and 25.6% for YT, and 1,267.5 U and 26.0% for AP. Gel filtration chromatography was more effective at fractionating PI than using ion exchange chromatography. These results suggest that fish eggs act as serine protease inhibitors and might be useful for protease inhibition in foodstuffs.

키워드

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피인용 문헌

  1. Recovery of serine protease inhibitor from fish roes by polyethylene glycol precipitation vol.19, pp.1, 2016, https://doi.org/10.1186/s41240-016-0016-x