초유은행에서 수거한 초유의 병원체 오염과 면역수준의 평가

Assessment of Immune Quality and Pathogen Contamination of Colostrums Collected from Colostrum Banks in Korea

  • Kim, Won-Il (College of Veterinary Medicine, Chonbuk National University) ;
  • Park, Sang-Yul (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Sang-Jin (College of Veterinary Medicine, Chonbuk National University) ;
  • Cho, Yong-Il (National Institute of Animal Science, Rural Development Administration) ;
  • Hur, Tai-Young (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Nam-Soo (College of Veterinary Medicine, Chonbuk National University)
  • 심사 : 2013.08.21
  • 발행 : 2013.08.31

초록

송아지는 초유를 통해서만 모체항체를 받을 수 있기 때문에 신생우가 분만초기에 초유를 섭취하는 것은 질병에 대한 저항성을 갖추기 위해 필수적이다. 따라서 분만 후 잉여 초유를 저장하였다가 초유섭취가 부족한 신생우에 공급하여 충분한 항체를 갖도록 하는 것이 일반적인 사양법이다. 본 연구에서는 국내 3곳의 지역에서 운영되는 초유은행에서 저장된 35개의 초유를 무작위로 수거하여 일반세균오염과 주요 병원체들을 검사하였고 면역글로불린의 농도와 BVDV 특이 항체가를 측정하여 초유의 면역학적 품질을 평가하였다. 초유은행에서 수거된 대부분의 초유에서 중등도에서 고도의 세균오염이 관찰되었다. 또한 대부분의 초유가 좋은 품질의 초유라고 판단되는 50 g/L의 IgG 농도에 미치지 못하는 IgG를 포함하고 있었다. 따라서 초유은행 보관용 초유는 청결한 채취법에 따라 분만 후 2-3회 이하로 착유한 초유를 사용하여 안전성과 품질을 보장하여야 할 것이다. 또한 $63^{\circ}C$에서 30분간 실시하는 저온살균이 면역글로불린의 파괴를 최소화하는 동시에 초유에 의한 질병전파를 확연히 줄여주는 것으로 관찰되었으므로 냉동보관 전에 초유의 저온살균 과정을 반드시 실시하는 것이 필요할 것으로 판단된다.

Because colostrum is considered to be the sole source of passively acquired maternal antibodies for calves, newborn calves must consume colostrum to gain disease resistance during their early years of life. Storage of surplus colostrum from dairy cows right after calving and feeding newborn calves in deficiency of colostrum to assure adequate uptake of IgG for protection of the calf has been a common practice in the bovine production. In the current study, 35 colostrums were randomly collected from 3 colostrum banks located in different regions of Korea and monitored for general bacterial contamination and major bovine pathogens. Immunoglobulin concentrations and BVDV-specific antibodies were also determined to evaluate the immune quality of the colostrums. Moderate to severe bacterial contamination (up to 72,000,000 CFU/ml) was observed in most of the colostrums collected from colostrum banks. General immune quality of the colostrums was under the satisfactory level since most of the colostrums contained less than 50 g/L of IgG, which is the minimum concentration for good quality colostrums. Therefore, colostrum for colostrum bank should be collected at the first 2-3 post-partum milkings according to proper harvesting and handling procedures to guarantee the safety and quality of colostrum. In addition, it was recommended that colostrum should be heat-treated before frozen and stored in the bank because pasteurization at $63^{\circ}C$ for 30 min was very effective reducing the risk of disease transmission without causing significant degradation of immunoglobulins.

키워드

참고문헌

  1. Acha SJ, Kuhn I, Jonsson P, Mbazima G, Katouli M, Mollby R. Studies on calf diarrhoea in Mozambique: prevalence of bacterial pathogens. Acta Vet Scand 2004; 45: 27-36. https://doi.org/10.1186/1751-0147-45-27
  2. Calmettes P, Cser L, Rajnavolgy E. Temperature and pH dependence of immunoglobulin G conformation. Arch Biochem Biophys 1991; 291: 277-283. https://doi.org/10.1016/0003-9861(91)90135-6
  3. Chen C-C, Chang H-M. Effect of thermal protectants on the stability of bovine milk immunoglobulin G. J Agric Food Chem 1998; 46: 3570-3576. https://doi.org/10.1021/jf970980f
  4. Chigerwe M, Tyler JW, Schultz LG, Middleton JR, Steevens BJ, Spain JN. Effect of colostrum administration by use of oroesophageal intubation on serum IgG concentrations in Holstein bull calves. Am J Vet Res 2008; 69: 1158-1163. https://doi.org/10.2460/ajvr.69.9.1158
  5. Cho YI, Kim WI, Liu S, Kinyon JM, Yoon KJ. Development of a panel of multiplex real-time polymerase chain reaction assays for simultaneous detection of major agents causing calf diarrhea in feces. J Vet Diagn Invest 2010; 22: 509-517. https://doi.org/10.1177/104063871002200403
  6. Dominguez E, Perez MD, Calvo M. Effect of heat treatment on the antigen-binding activity of anti-peroxidase immunoglobulins in bovine colostrum. J Dairy Sci 1997; 80: 3182- 3187. https://doi.org/10.3168/jds.S0022-0302(97)76290-8
  7. Godden S, McMartin S, Feirtag J, Stabel J, Bey R, Goyal S, Metzger L, Fetrow J, Wells S, Chester-Jones H. Heattreatment of bovine colostrum. II: effects of heating duration on pathogen viability and immunoglobulin G, J Dairy Sci 2006; 89: 3476-3483. https://doi.org/10.3168/jds.S0022-0302(06)72386-4
  8. Herrera-Rodriguez SE, Gordiano-Hidalgo MA, Lopez-Rincon G, Bojorquez-Narvaez L, Padilla-Ramirez FJ, Pereira-Suarez AL, Flores-Valdez MA, Estrada-Chavez C. Mycobacterium bovis DNA detection in colostrum as a potential indicator of vaccination effectiveness against bovine tuberculosis. Clin Vaccine Immunol 2013; 20: 627-633. https://doi.org/10.1128/CVI.00566-12
  9. Hurley WL, Theil PK. Perspectives on immunoglobulins in colostrum and milk. Nutrients 2011; 3: 442-474. https://doi.org/10.3390/nu3040442
  10. Jong SK, Gong SK, Chung HK, Dae SH. Dairy-cattle health in Gyeongnam, Korea. Prev Vet Med 2001; 52: 163-169. https://doi.org/10.1016/S0167-5877(01)00215-X
  11. Li-Chan E, Kummer A, Loso JN, Kitts DD, Nakai S. Stability of bovine immunoglobulins to thermal treatment and processing. Food Res Int 1995; 28: 9-16. https://doi.org/10.1016/0963-9969(95)93325-O
  12. Mainer G, Sanchez L, Ena JM, Calvo M. Kinetic and thermodynamic parameters for heat denaturation of bovine milk IgG, IgA and IgM. J Food Sci 1997; 62: 1034-1038. https://doi.org/10.1111/j.1365-2621.1997.tb15032.x
  13. Mishra A, Singhal A, Chauhan DS, Katoch VM, Srivastava K, Thakral SS, Bharadwaj SS, Sreenivas V, Prasad HK. Direct detection and identification of Mycobacterium tuberculosis and Mycobacterium bovis in bovine samples by a novel nested PCR assay: correlation with conventional techniques. J Clin Microbiol 2005; 43: 5670-5678. https://doi.org/10.1128/JCM.43.11.5670-5678.2005
  14. Radwan GS, Brock KV, Hogan JS, Smith KL. Development of a PCR amplification assay as a screening test using bulk milk samples for identifying dairy herds infected with bovine viral diarrhea virus. Vet Microbiol 1995; 44: 77-91. https://doi.org/10.1016/0378-1135(94)00121-C
  15. Reynolds DJ, Morgan JH, Chanter N, Jones PW, Bridger JC, Debney TG, Bunch KJ. Microbiology of calf diarrhoea in southern Britain. Vet Rec 1986; 119: 34-39. https://doi.org/10.1136/vr.119.2.34
  16. Saif LJ, Smith KL. Enteric viral infections of calves and passive immunity. J Dairy Sci 1985; 68: 206-228. https://doi.org/10.3168/jds.S0022-0302(85)80813-4