Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Duration of Preservation Affect the Quality of Chilled Black Bengal Buck Semen

  • Pradhan, Md. Gulshan Anowar (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Rahman, Md. Saidur (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Kwon, Woo-Sung (Department of Animal Science & Technology, School of Bioresource & Bioscience, Chung-Ang University) ;
  • Mishra, Dipendra (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Kamal, Md. Mostofa (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Bhuiyan, Mohammad Musharraf Uddin (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Shamsuddin, Mohammed (Department of Surgery and Obstetrics, Bangladesh Agricultural University)
  • Received : 2013.04.28
  • Accepted : 2013.05.14
  • Published : 2013.06.30
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Abstract

The study focuses on the quality assessment of Black Bengal buck semen preserved at chilled condition. In this in vitro trial, collected semen from Black Bengal bucks was preserved at chilling temperature ($4{\sim}5^{\circ}C$) in tris-glucosecitrate yolk medium of 1:5 ratios for four days. Artificial Vagina (AV) method was utilized to collect semen from buck. General evaluation of semen includes the color, mass activity and density were measured by direct visual examination. However, computer-assisted sperm analysis (CASA) and phase contrast microscopy were used to figure out the motility (%), hyper-activated (HYP) motility (%) and number of abnormal spermatozoa (%) initially, and at every 24 h intervals. The result revealed that spermatozoa preserved at chilling temperature showed significantly (P<0.05) lower motility and HYP motility with the progression of preservation. The number of phenotypically abnormal spermatozoa significantly (P<0.05) increased following preservation. Although significant positive correlation (r=0.945; P<0.05) was existed between % motile and % HYP motile spermatozoa however, the % of morphologically abnormal spermatozoa was negatively correlated with % motile (r=-0.997; P<0.05) and % HYP motile spermatozoa (r=-0.946; P<0.01). Therefore, we concluded that the quality of chilled semen progressively losses its viability and doesn't remain useable after certain period of preservation with respect to its motility and morphology.

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References

  1. Apu AS. 2012. Fertility of Fresh and Frozen-thawed Black Bengal Goat Spermatozoa: Fertility of Fresh and FrozenThawed Goat Semen. 1st ed, Lambert Academic Publishing, Germany, pp. 1-104.
  2. Bakshi S, Patil AV, Srivas AK, Jagtap DZ and More BK. 1987. Studies on semen evaluation and fertility rate of Angra and 7/8 Angra bucks. Livestock Adviser 12: 13-18.
  3. Farrell PB, Presicce GA, Brockett CC and Foote RH. 1998. Quantification of bull sperm characteristics measured by computer-assisted sperm analysis (CASA) and the relationship to fertility. Theriogenology 49: 871-879. https://doi.org/10.1016/S0093-691X(98)00036-3
  4. Herman HA and Madeen FW. 1963. The Artificial Insemination of Dairy Cattle and Beef Cattle: A Hand Book and Laboratory Manual. Locas Brothers, Columbia, pp: 21-41.
  5. Itach SBS, Finklestein M, Etkovitz N and Breitbart H. 2001. Hyper-activated motility in sperm capacitation is mediated by phospholipase D-dependent actin polymerization. Dev. Biol. 362: 154-161.
  6. Karagiannidis AS, Varsakeli and Karatzas G. 2000. Characteristics and seasonal variations in the semen of Alpine, Saanen and Damascas goat bucks born and raised in Greece. Theriogenology 53: 1285-1293. https://doi.org/10.1016/S0093-691X(00)00272-7
  7. Karatzas GA, Karagiannidis Varsaleli S and Brikas P. 1997. Fertility of fresh and frozen thawed goat semen during the non-breeding season. Theriogenology 48: 1049-1059. https://doi.org/10.1016/S0093-691X(97)00331-2
  8. Leboeuf BB, Restall and Salamon S. 2000. Production and storage of goat semen for artificial insemination, Anim. Reprod. Sci. 62: 113-141. https://doi.org/10.1016/S0378-4320(00)00156-1
  9. Leon H, Porras AA, Galina CS and Navarro-Fierro R. 1991. Effect of the collection method on semen characteristics of Zebu and European type cattle in the tropics, Theriogenology 36: 349-355. https://doi.org/10.1016/0093-691X(91)90463-N
  10. Love CC. 2011. Relationship between sperm motility, morphology and the fertility of stallions. Theriogenology 76: 547- 557. https://doi.org/10.1016/j.theriogenology.2011.03.007
  11. Marx D, Klempp K and Bohmm S. 1975. Gynaecomastia in a buck. Sexual activity, semen quality and milk quality. Zuchthygiene 10: 125-135. https://doi.org/10.1111/j.1439-0531.1975.tb00291.x
  12. Medeiros CMO, Forell F, Oliveira ATD and Rodrigues JL. 2002. Current status of sperm cryopreservation: Why isn't it better? Theriogenology 57:327-44. https://doi.org/10.1016/S0093-691X(01)00674-4
  13. O'Connel M, McClure N and Lewis SEM. 2002. The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Hum. Reprod. 17:704-9. https://doi.org/10.1093/humrep/17.3.704
  14. Ozkavukcu S, Erdemli E, Isik A, Oztuna D and Karahuseyinoglu S. 2008. Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. J. Assist. Reprod. Genet. 25:403-11. https://doi.org/10.1007/s10815-008-9232-3
  15. Quill AT and Garbers LD. 2002. Sperm motility activation and chemoattraction. In: Fertilization, Hardy, D.M. (Ed.). Academic Press, Carlifornia, pp: 29-29.
  16. Rahman MS, Shohag AS, Kamal MM, Bari FY and Shamsuddin M. 2012a. Preovulatory follicular and subsequent luteal size influence pregnancy success in water buffaloes. J. Reprod. Dev. 58:219-22. https://doi.org/10.1262/jrd.11-111T
  17. Rahman MS, Shohag AS, Kamal MM, Parveen N and Shamsuddin M. 2012b. Application of ultrasonography to investigate postpartum anestrus in water buffaloes. Reprod. Dev. Biol. 36:103-108.
  18. Rodriguez-Martin E, de la Hoz MF, Dura Ros MJ and Blanco CF. 1994. Evaluating the patient with orthopedic problems. Rev. Enferm. 17: 68-71.
  19. Shamsuddin M and Rodriguez-Martinez H. 1994. Fine structure of bovine blastocysts developed either in serum-free medium or in conventional co-culture with oviduct epithelial cells. Zentralbl. Vet. Med. Ser. A. 41: 307-316. https://doi.org/10.1111/j.1439-0442.1994.tb00097.x
  20. Singh DK, Sinha MP, Singh RA, Singh CSP and Singh KK. 1985. Comparative study on seminal quality of pure and cross-bred bucks. Indian Vet. Med. J. 9: 50-58.
  21. Sugulle AH, Bhuiyan MMU and Shamsuddin M. 2006. Breeding soundness of bulls and the quality of their frozen semen used in cattle artificial insemination in Bangladesh. Livestock Research for Rural Development 18: Article #54.
  22. Sullivan JJ. 1978. Morphology and motility of spermatozoa. In: Physiology of Reproduction and Artificial Insemination of Cattle. Salisbury, G. M., VanDemark, K. I. and Lodge, J. R. (eds.) Freman and Company, Sanfranciscio, USA, pp. 286-320.
  23. Suttiyotin P and Thwaites CJ. 1993. Evaluation of ram semenmotility by a swim-up technique. J. Reprod. Fertil. 97: 339-345. https://doi.org/10.1530/jrf.0.0970339
  24. Viller D and Salamon S. 1993. Fertility following inseminations frozen-thawed reconcentrated and unconcentrated ram semen. Australian J. Biol. Sci. 27: 423-426.
  25. Wise T, Lunstra DD, Rohrer GA and Ford JJ. 2003. Relationships of testicular iron and ferritin concentrations with testicular weight and sperm production in boars. J. Anim. Sci. 81: 503-511.
  26. Yanagimachi R. 1994. Mammalian fertilization. In "The Physiology of Reproduction" (E. Knobil and J. D. Neill, eds.), Raven Press, New York, pp. 189-317.
  27. Zamfirescu S and Nadolu D. 2009. Results concerning the freezing probability of buck semen and fecundity after artificial insemination of local goat. Lucrari Stiintifice Univ. de Stiinte Agricole si Medicina Veterinara, Seria Zootehnie 52: 80-84.
  28. Zhao BT, Han D, Xu CL, Luo MJ, Chang ZL and Tan JH. 2009. Protocol optimization for long-term liquid storage of goat semen in a chemically defined extender. Reprod. Domest. Anim. 44:865-872. https://doi.org/10.1111/j.1439-0531.2008.01101.x