Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Saussurea Lappa Radix-induced cytotoxicity via ROS generation in A549 lung cancer cells

A549세포에 대한 목향추출물의 ROS 매개 세포독성

  • Lee, Young-Joon (Department of Preventive Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Ku, Sae-Kwang (Medical Research Center for Globalization of Herbal Formulation, Daegu Haany University) ;
  • Kang, Su-Jin (Department of Preventive Medicine, College of Korean Medicine, Daegu Haany University)
  • 이영준 (대구한의대학교 한의과대학 예방의학교실) ;
  • 구세광 (대구한의대학교 방제과학글로벌연구센터) ;
  • 강수진 (대구한의대학교 한의과대학 예방의학교실)
  • Received : 2013.08.01
  • Accepted : 2013.08.28
  • Published : 2013.08.31
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Abstract

Objectives : Many cancers acquired resistance to chemotherapy, thus limiting its anticancer efficacy. It is known that Glutathione (GSH) is related to the development of drug resistance. The expression of GSH synthesizing glutamylcysteine ligase (GCL) was controlled by nuclear factor-E2-related factor(Nrf2). Previous studies showed that pharmacological depletion of GSH results in ROS increase, apoptotic response, and sensitization to oxidizing stimuli. In the current study, we examined Saussurea Lappa (SL) have the inhibitory effect on Nrf2 activity using human lung cancer A549 cells overexpressing Nrf2. Methods : Cell viability of A549 cells on SL treatment was determined by MTT assay. To detect the apeptosis in SL-treated A549 cells, sub-G1 population was measured by flow cytometry analysis (FACS). The level ROS was determined by FACS and fluorescence microscopy. To investigate whether SL have effect the suppression on Nrf2, we performed western blotting analysis. The GSH content was measured since GSH plays an important role in response to oxidative stress and was regulated by Nrf2. Results : A549 cells treated with an SL extract showed a substantial decrease in cell viability, along with a concomitant increase in apoptosis compared to untreated cells. Treatment of the SL extract led to increased Reactive oxygen species (ROS) production and a suppression of Nrf2. In addition, the antioxidant NAC attenuated SL-induced ROS generation, Nrf2 inhibition, and apoptosis. Taken together, these data show that the SL extract induced the production of ROS, and the inhibition of Nrf2, consequently resulting in A549 cell death. Conclusions : These results suggest that SL might be an effective agent to enhance anticancer drug sensitivity via Nrf2 inhibition.

Keywords

References

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