YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Targeted Protein Expression in Freshly Isolated Vascular Tissues by Using Adenoviral Vector

생체에서 분리된 혈관조직에서 아데노바이러스벡터를 이용한 특정 단백질의 발현

  • Huh, Yang Hoon (Division of Electron Microscopic Research, Korea Basic Science Institute) ;
  • Kim, Hak Rim (Department of Pharmacology, College of Medicine, Dankook University)
  • 허양훈 (한국기초과학지원연구원 전자현미경연구부) ;
  • 김학림 (단국대학교 의과대학 약리학교실)
  • Received : 2013.06.14
  • Accepted : 2013.07.18
  • Published : 2013.08.31
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Abstract

Treatments of vascular disease via modulating the expression of specific proteins by gene transfer have been attempted in various studies over the past few years. Among several methods to deliver genes, adenovirus currently has been used because of a number of positive aspects. In this study, we test adenoviral vector as a potential mediator in the treatment of vascular disease by using freshly isolated vascular tissues not cultured vascular cells. Freshly isolated vascular tissues were directly exposed to adenoviral vector pAd5CMVmcsIRESeGFPpA to check the possibility of GFP expression in different layer of vascular tissues. We found that the GFP expression by using adenoviral vector experiments is mainly focused on the adventitia and failed to detect GFP expression at endothelial layer or vascular smooth muscle layer in vascular tissues. However, we also found that several integrin receptors are robustly expressed in vascular smooth muscle, thus the limited expression of protein in vascular smooth muscle are not likely the lack of integrin receptors. In conclusion, adenovirus could not be a good tool for a specific protein expression in vascular smooth muscle cell. Thus, the application of adenovirus as a tool for gene therapy of vascular smooth muscle cells in clinical therapeutic trial need to be optimized further.

Keywords

References

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