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Biocidal effect to fish pathogens of Aqua farmsafe® composed of yucca extract and didecyldimethylammonium chloride

유카추출물과 didecyldimethylammonium chloride를 주성분으로 하는 살균소독제 아쿠아 팜세이프의 어류병원체에 대한 살균 효과

  • Seo, Jung Soo (Pathology Division, National Fisheries Research & Development Institute (NFRDI)) ;
  • Jeon, Eun Ji (Pathology Division, National Fisheries Research & Development Institute (NFRDI)) ;
  • Hwang, Jee Youn (Pathology Division, National Fisheries Research & Development Institute (NFRDI)) ;
  • Jung, Sung Hee (Pathology Division, National Fisheries Research & Development Institute (NFRDI)) ;
  • Park, Myoung Ae (Aquatic Life Disease Control Division, NFRDI) ;
  • Lee, Sung Min (Woosung Co. Ltd.) ;
  • Lee, Eun Hye (Pathology Division, National Fisheries Research & Development Institute (NFRDI))
  • 서정수 (국립수산과학원 병리연구과) ;
  • 전은지 (국립수산과학원 병리연구과) ;
  • 황지연 (국립수산과학원 병리연구과) ;
  • 정승희 (국립수산과학원 병리연구과) ;
  • 박명애 (국립수산과학원 수산생물방역과) ;
  • 이성민 ((주)우성양행) ;
  • 이은혜 (국립수산과학원 병리연구과)
  • Received : 2013.04.08
  • Accepted : 2013.08.09
  • Published : 2013.08.30

Abstract

In this study, the disinfectant efficacy of Aqua farmsafe$^{(R)}$, composed of didecyldimethylammonium chloride (DDAC) and yucca extract was evaluated against Salmonella typhimurium and fish pathogens. Determination of the anti-microbial or anti-viral efficacy of the disinfectant was based on Animal, Plant and Fisheries Quarantine and Inspection Agency Regulation No. 2011-26, Korea. Anti-bacterial efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at $4^{\circ}C$. Aqua farmsafe and test bacteria or virus were diluted with distilled water (DW), standard hard water (SW) or organic matter dilution (OM) according to treatment condition. Under the our results, disinfectant efficacy of Aqua farmsafe$^{(R)}$ possesses 30~40 fold against fish pathogens including bacteria and virus compared to that on animal pathogenic bacteria, S. typhimurim. As the efficacy of Aqua farmsafe$^{(R)}$ against fish pathogen was investigated in vitro, a controlled field trial is required to determine whether the use of Aqua farmsafe$^{(R)}$ will be able to reduce fish diseases.

본 연구에서는 4급 암모늄에 속하는 didecyldimethylammonium chloride (DDAC)와 유카추출물 (Yucca extract)의 합제인 아쿠아 팜세이프$^{(R)}$의 어류 질병 병원체에 대한 살균효과를 확인하기 위하여 농림수산검역검사본부의 소독제 효력시험 중 세균 및 바이러스 소독제 효력시험에 따라 수행하였다. 소독제와 병원체를 증류수, 경수 그리고 유기물 조건에서 반응시킨 후, 중화액을 이용하여 중화시킨 다음, 중화된 용액을 배지 및 주화세포에서 접종시켜 세균의 성장 및 세포변성효과를 통해 소독제의 효력배수를 결정하였다. 본 연구의 결과, 아쿠아 팜세이프$^{(R)}$는 식중독을 일으키는 공시균주 살모넬라균 (S. typhimurim)에 비하여, 어병세균이 30~40배 이상 높은 희석농도에서 소독효과를 나타내었으며, VHSV에 대하여서는 2,000~3,600배의 유효한 효력배수를 나타내었다. 따라서, 향후, 야외적용시험을 통해 실제 적용에 따른 효과를 확인할 필요가 있을 것으로 사료된다.

Keywords

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