The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-inflammatory effects of Lespedeza Cuneata in vivo and in vitro

야관문(夜關門)의 항염증효과(抗炎症效果)

  • Lee, Hoil (Daegu Haany University, College of Oriental Medicine, Dept. of ophthalmology, otolaryngology and dermatology) ;
  • Jung, Ji Yun (Daegu Haany University, College of Oriental Medicine, Dept. of Herbal formula) ;
  • Hwangbo, Min (Daegu Haany University, College of Oriental Medicine, Dept. of ophthalmology, otolaryngology and dermatology) ;
  • Ku, Sea Kwang (Daegu Haany University, College of Oriental Medicine, Dept. of Anatomy) ;
  • Kim, Young Woo (Daegu Haany University, College of Oriental Medicine, Dept. of Herbal formula) ;
  • Jee, Seon Young (Daegu Haany University, College of Oriental Medicine, Dept. of ophthalmology, otolaryngology and dermatology)
  • 이호일 (대구한의대학교 한의과대학 외관과학교실) ;
  • 정지윤 (대구한의대학교 한의과대학 방제학교실) ;
  • 황보민 (대구한의대학교 한의과대학 외관과학교실) ;
  • 구세광 (대구한의대학교 한의과대학 해부학교실) ;
  • 김영우 (대구한의대학교 한의과대학 방제학교실) ;
  • 지선영 (대구한의대학교 한의과대학 외관과학교실)
  • Received : 2013.04.28
  • Accepted : 2013.07.30
  • Published : 2013.07.30
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Abstract

Objectives : Lespedeza Cuneata has been used to treat leukorrhea, asthma, stomach pain, diarrhea, acute mastitis, in Korean traditional medicine. According to recent studies, Lespedeza Cuneata has antioxidation, hypoglycemia, cell protective, insulin secretion, whitening, corpora cavernosa smooth muscle relaxation and antimicrobial activities, but it has been rarely conducted to evaluate the immuno-biological activity. The present study was examined to evaluate the anti-inflammatory effects of the Lespedeza Cuneata MeOH extract (LCE) in vivo and in vitro. Methods : In vitro, inflammatory mediators, such as cytokines, nitric oxide and prostaglandin $E_2$ were detected after the addition of LPS with or without LCE in Raw 264.7 macrophage cell line. In vivo, anti-edema effect of LCE was determined in the carrageenan-induced paw edema model in rats. Results : In vitro assay, LCE decreased release of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) via suppression of iNOS and COX-2 expression. LCE inhibited the phosphorylation of $I{\kappa}B$ indicating the suppression of NF-${\kappa}B$ pathway. In vivo assay, LCE significantly inhibited the formation of paw edema induced by carrageenan injection in rats. LCE effectively inhibited increases of hind paw skin thickness and inflammatory cell infilterations. Conclusion : These findings demonstrate that LCE has inhibitory effect on inflammatory mediators in LPS-activated Raw 264.7 cells and on paw edema in carrageenan-stimulated rats, showing the possibility of anti-inflammatory use of Lespedeza Cuneata.

Keywords

References

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